MCM2-7 ring closure involves the Mcm5 C-terminus and triggers Mcm4 ATP hydrolysis.

The eukaryotic helicase MCM2-7, is loaded by ORC, Cdc6 and Cdt1 as a double-hexamer onto replication origins. The insertion of DNA into the helicase leads to partial MCM2-7 ring closure, while ATP hydrolysis is essential for consecutive steps in pre-replicative complex (pre-RC) assembly. Currently it is unknown how MCM2-7 ring closure and ATP-hydrolysis are controlled.

Evaluation of the therapeutic relevance of architectural aspects in child and adolescent psychiatric institutions from the perspective of architects and senior physicians.

Objective: The concept of "Healing Architecture" addresses the relevance of design and architectural issues on the outcome of medical and therapeutic treatment in hospitals. The questionnaire ARCHI was developed to record data on the opinion of different groups of users on the architectural design of their therapeutic environment in departments of child and adolescent psychiatry.

Method: A Questionnaire-based cross-sectional study was conducted in two phases between 2020 and 2022 using ARCHI to gather the perspectives of senior physicians and architects on the significance of architectural design in German child and adolescent psychiatric facilities.

Analysis of cell cycle stage, replicated DNA, and chromatin-associated proteins using high-throughput flow cytometry.

Flow cytometry is a versatile tool used for cell sorting, DNA content imaging, and determining various cellular characteristics. With the possibility of high-throughput analyses, it combines convenient labelling techniques to serve rapid, quantitative, and qualitative workflows. The ease of sample preparation and the broad range of applications render flow cytometry a preferred approach for many scientific questions.

Major Adverse Cardiovascular Events: The Importance of Serum Levels and Haplotypes of the Anti-Inflammatory Cytokine Interleukin 10.

Background: Cardiovascular diseases (CVDs) represent major medical and socio-economic challenges worldwide. There is substantial evidence that CVD is closely linked to inflammatory changes triggered by a complex cytokine network. In this context, interleukin 10 (IL-10) plays an important role as a pleiotropic cytokine with an anti-inflammatory capacity.

MCM2-7 loading-dependent ORC release ensures genome-wide origin licensing.

Origin recognition complex (ORC)-dependent loading of the replicative helicase MCM2-7 onto replication origins in G1-phase forms the basis of replication fork establishment in S-phase. However, how ORC and MCM2-7 facilitate genome-wide DNA licensing is not fully understood. Mapping the molecular footprints of budding yeast ORC and MCM2-7 genome-wide, we discovered that MCM2-7 loading is associated with ORC release from origins and redistribution to non-origin sites.

Evaluation of cell survival in different 3D-printed geometric shapes of human iPSC-derived engineered heart tissue.

Objectives: Engineered Heart Tissue (EHT) is a promising tool to repair heart muscle defects and can additionally be used for drug testing. Due to the absence of an in vitro vascularization, EHT geometry crucially impacts nutrient and oxygen supply by diffusion capacity. We analyzed cardiomyocyte survival in different EHT geometries.

Does an instructional video as a stand-alone tool promote the acquisition of practical clinical skills? A randomised simulation research trial of skills acquisition and short-term retention.

Background: The effectiveness of instructional videos as a stand-alone tool for the acquisition of practical skills is yet unknown because instructional videos are usually didactically embedded. Therefore, we evaluated the acquisition of the skill of a humeral intraosseous access via video in comparison to that of a self-study with an additional retention test.

Methods: After ethical approval, we conducted two consecutive studies.

Identifying a real space measure of charge-shift bonding with probability density analysis.

Charge-shift bonds have been hypothesized as a third type of chemical bonds in addition to covalent and ionic bonds. They have first been described with valence bond theory where they are identified by the resonance energy resulting from ionic contributions. While other indicators have been described, a clear real space fingerprint for charge-shift bonding is still lacking.

Reading and writing from right to left after anterior cerebral artery stroke.

This is the case report of a woman who started to write and read from right to left after anterior cerebral artery stroke, affecting the left supplementary motor area. No cases were found in the literature with exactly the same characteristics. She has been able to read and write faster after rehabilitation approach at Sarah Network of Rehabilitation Hospitals, in the Belo Horizonte city unit, Brazil, despite the maintenance of the inversion.

Associations between therapists' verbal techniques and patient-rated therapeutic alliance, insight, and problem solving.

Objective: To investigate associations between common factors (alliance, insight, problem solving) and therapists' techniques. We expected a positive association between (1) supportive techniques and the alliance, (2) interpretative techniques and insight, and (3) a stronger association between interpretative techniques and insight for patients with more severe baseline symptoms. Other associations were analyzed in an exploratory way.

Bionic Nanocoating of Prosthetic Grafts Significantly Reduces Bacterial Growth.

Prosthetic materials are a source of bacterial infections, with significant morbidity and mortality. Utilizing the bionic "Lotus effect," we generated superhydrophobic vascular prostheses by nanocoating and investigated their resistance to bacterial colonization. Nanoparticles were generated from silicon dioxide (SiO), and coated vascular prostheses developed a nanoscale roughness with superhydrophobic characteristics.

Hearing loss, depression, and cognition in younger and older adult CI candidates.

Background And Aim: Hearing loss in old age is associated with cognitive decline and with depression. Our study aimed to investigate the relationship between hearing loss, cognitive decline, and secondary depressive symptoms in a sample of younger and older cochlear implant candidates with profound to severe hearing loss.

Methods: This study is part of a larger cohort study designated to provide information on baseline data before CI.

Factors influencing the duration of untreated illness among patients with anorexia nervosa: A multicenter and multi-informant study.

Introduction: The duration of untreated illness (DUI), that is, the interval between the onset of anorexia nervosa (AN) symptoms and start of specialized treatments, has a strong influence on the prognosis.

Objective: To quantify modifiable predictors of the DUI and to derive recommendations for secondary prevention strategies.

Methods: Within a multicenter, multi-informant study, DUI was assessed in interviews with patients undergoing first specialized AN treatment.

Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis.

Objective: To determine the effects of sperm deoxyribonucleic acid (DNA) fragmentation at the time of fertilization on in vitro fertilization (IVF) outcomes and genetic diagnosis using next generation sequencing.

Design: Prospective double-blinded study.

Setting: Private Clinic.

Direct assessment of individual connotation and experience .

We introduce cognitive-affective maps (CAMs) as a novel tool to assess individual experiences and belief systems. CAMs were first presented by the cognitive scientist and philosopher Paul Thagard as a graphical representation of a mental network, visualizing attitudes, thoughts, and affective connotations toward the topic of interest. While CAMs were originally used primarily to visualize existing data, the recent release of the new software tool has facilitated the applicability of CAMs for empirical data collection.

Co-pigmentation of strawberry anthocyanins with phenolic compounds from rooibos.

Anthocyanin-rich strawberry model solutions were co-pigmented with rooibos phenolics to enhance color and heat stability. The addition of green and fermented rooibos extracts at pigment-to-co-pigment molar ratios of 1:10, 1:50, and 1:100 pelargonidin-3-glucoside equivalents: orientin equivalents induced hyper- and bathochromic shifts at room temperature and during thermal processing at 80 °C for an hour. Co-pigmentation effects on hyperchromic shift were up to 96%, and bathochromic shift reached 19 nm when adding flavonoid-rich fractions of green rooibos phenolics.

Multi-center bonds as resonance hybrids: A real space perspective.

The concept of distinct bonds within molecules has proven to be successful in rationalizing chemical reactivity. However, bonds are not a well-defined physical concept, but rather vague entities, described by different and often contradicting models. With probability density analysis, which can-in principle-be applied to any wave function, bonds are recovered as spin-coupled positions within most likely electron arrangements in coordinate space.

Internet-Based Prevention Program of Victimization for Youth in Care and Care Leavers (EMPOWER YOUTH): Protocol for a Randomized Controlled Trial.

Background: The global estimate of the number of children in institutional care is around 5 million, with around 1 million of these children living in Europe. In Germany, about 75,000 children and adolescents find themselves in the foster care system and about 93,000 additional children and adolescents are living in institutions. Traumatic experiences and neglect in childhood are highly prevalent among these youth in care and are related to severe long-term effects.

[ARCHI - Development of a questionnaire on architecture of child and adolescent psychiatric facilities].

ARCHI - Development of a questionnaire on architecture of child and adolescent psychiatric facilities Studies from various disciplines have pointed to a connection between the structural characteristics of hospitals, in particular psychiatric hospital wards, and various parameters in the recovery process of patients treated there. To date, however, no current studies have further investigated whether individual architectural aspects of clinical complexes are more relevant in everyday practice than others. To link theory with practice in the context of this study, we developed a questionnaire to explore which architectural aspects in hospitals or utilities for child and adolescent psychiatry, psychosomatics, and psychotherapy are perceived by those working in therapy as helpful or detrimental to therapeutic treatment.

The development and psychometric evaluation of FABIANA-checklist: a scale to assess factors influencing treatment initiation in anorexia nervosa.

Background: A long duration of untreated illness (DUI) is an unfavorable prognostic factor in anorexia nervosa (AN) and is associated with chronic illness progression. Although previous preventive measures aimed at reducing DUI and thus improving short- and long-term treatment outcomes have been partially successful, a better understanding of the factors involved in the sensitive phase prior to treatment initiation is needed. To date, there is no validated instrument available to assess these factors specifically for patients with AN.

Light-triggered and phosphorylation-dependent 14-3-3 association with NON-PHOTOTROPIC HYPOCOTYL 3 is required for hypocotyl phototropism.

NON-PHOTOTROPIC HYPOCOTYL 3 (NPH3) is a key component of the auxin-dependent plant phototropic growth response. We report that NPH3 directly binds polyacidic phospholipids, required for plasma membrane association in darkness. We further demonstrate that blue light induces an immediate phosphorylation of a C-terminal 14-3-3 binding motif in NPH3.

Real space electron delocalization, resonance, and aromaticity in chemistry.

Chemists explaining a molecule's stability and reactivity often refer to the concepts of delocalization, resonance, and aromaticity. Resonance is commonly discussed within valence bond theory as the stabilizing effect of mixing different Lewis structures. Yet, most computational chemists work with delocalized molecular orbitals, which are also usually employed to explain the concept of aromaticity, a ring delocalization in cyclic planar systems which abide certain number rules.

A Novel Network Approach to Capture Cognition and Affect: COVID-19 Experiences in Canada and Germany.

We tested a novel method for studying human experience (thoughts and affect). We utilized Cognitive-Affective Maps (CAMs)-an approach to visually represent thoughts and their affective connotations as networks of concepts that individuals associate with a given event. Using an innovative software tool, we recruited a comparative sample of ( = 93) Canadians and ( = 100) Germans to draw a CAM of their experience (events, thoughts, feelings) with the Covid-19 pandemic.