Genetic characterization of -acetyltransferase 2 variants in acquired multidrug-resistant tuberculosis in Indonesia.

Owing to the high resistance rate of tuberculosis (TB) to isoniazid, which is metabolized by -acetyltransferase 2 (NAT2), we investigated the associations between variants and multidrug-resistant (MDR)-TB. The acetylator status based on haplotypes of 128 patients with MDR-TB in Indonesia were compared with our published data from patients with anti-TB drug-induced liver injury (AT-DILI), TB and the general population. was more frequent in the MDR-TB group than in the AT-DILI group, TB controls and general controls.

NAT2 slow acetylator is associated with anti-tuberculosis drug-induced liver injury severity in indonesian population.

We investigated the contribution of NAT2 variants and acetylator status to anti-tuberculosis drug-induced liver injury (AT-DILI) severity. 100 patients with clinically severe AT-DILI and 210 non-AT-DILI controls were subjected to NAT2 genotyping by direct DNA sequencing. NAT2 slow acetylator was significantly associated with AT-DILI risk (p = 2.

N-acetyltransferase 2 polymorphism and acetylation profiles in Buginese ethnics of Indonesia.

Background: N-acetyltransferase 2 (NAT2) is a key enzyme involved in the phase II metabolism of aromatic amines and heterocyclic aromatic amines present in a wide range of xenobiotics. The aim of this study was to investigate the NAT2 polymorphism in the Buginese ethnic group of Indonesia to determine the frequency of NAT2 alleles in this population.

Results: We found six haplotypes consisting of six single-nucleotide polymorphisms and 12 NAT2 genotype variations.

NAT2 variants are associated with drug-induced liver injury caused by anti-tuberculosis drugs in Indonesian patients with tuberculosis.

Drug-induced liver injury (DILI) is the most common adverse drug reaction in the treatment of tuberculosis (TB). Several studies showed that patients with TB and the slow-acetylator phenotype caused by NAT2 variants are highly susceptible to DILI caused by anti-TB drugs, hereafter designated AT-DILI. However, the role of NAT2 variants in AT-DILI has never been assessed for an Indonesian population.

Normal value for the direct micronucleus test on peripheral blood mononuclear cells from healthy donors.

Objective: To obtain the normal value of micronuclei in peripheral blood mononuclear cells.

Study Design: We screened 300 blood samples for micronucleated cells. Samples from donors who smoked, were ill or lived in a polluted area were excluded.