The incidence of post-operative deep vein thrombosis in general surgical patients of hospital universiti sains malaysia.

The incidence of deep vein thrombosis is believed to be rare in Asians. During recent years, a few reports have appeared with high incidence in orthopaedic patients comparable to Western studies. This study was carried out to find out the incidence of post-operative deep vein thrombosis (DVT) after major surgical procedures in general surgical patients in an Asian population.

Test protocol of an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against bovine leukosis virus.

In this communication the test procedure is described for an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against bovine leukosis virus (BLV). Test sera are incubated in polystyrene microtiter plates sensitized with a partly-purified preparation of BLV. Bovine antibodies are detected with anti-species immunoglobulin conjugated to the enzyme horseradish peroxidase, followed by the addition of the enzyme substrate.

Studies on bovine leukosis. VII. Further experience with an ELISA for the detection of antibodies to bovine leukosis virus.

An enzyme linked immunosorbent assay (ELISA) and the agar gel immunodiffusion test with bovine leukosis virus glycoprotein as antigen (AGIDT-BLV gp) were further used to test 633 bovine sera for antibodies to BLV. Both tests detected the same number of sera positive (149) or negative (464) for antibodies. Nine sera were negative in the ELISA but found to be weakly positive (2 sera) or bending the control line (7) in the AGDT-BLV gp.

Horse anti-bovine lymphocyte serum. Its effect in calves.

Anti-bovine lymphocyte serum (ABLS) had been prepared in horses with calf thymocytes as antigen and its effects in calves following parenteral administration were studied. The optimal dose was found to be one ml/kg body weight. The ABLS suppressed both the T and B cell functions.

Horse anti-bovine lymphocyte serum. Its effect in calves.

Summary Anti-bovine lymphocyte serum (ABLS) had been prepared in horses with calf thymocytes as antigen and its effects in calves following parenteral administration were studied. The optimal dose was found to be one ml/kg body weight. The A BLS suppressed both the T and B cell functions.

[Transmission and immune response in bovine enzootic leukosis (author's transl)].

The causative agent of enzootic bovine leukosis (EBL) is an oncogenic RNA virus named bonvine leukosis virus (BLV). At present, instead of bovine leukemia, the name bovine leukosis is preferentially used to avoid an erroneous association with leucaemia in man. In all European Community Countries the serological diagnosis of EBL has gradually replaced hematology.

Studies on bovine leucosis V. A comparative study on the practical value of the agar gel immunodiffusion test, the indirect fluorescent antibody technique and the micro complement fixation test for the detection of antibodies to bovine leucosis virus.

The practical value of the indirect fluorescent antibody technique (I FAT), the micro complement fixation test (M CFT) and the agar gel immunodiffusion test (AGIDT) for the detection of antibodies to bovine leucosis virus (BLV) was investigated. For this purpose 1495 serum samples were examined. There was a remarkably good agreement between the three tests in the demonstration of antibodies to BLV especially if sera with a sufficient high concentration of antibodies ("late serum") were under investigation.

Studies on bovine leukosis. VI. Enzyme linked immunosorbent assay for the detection of antibodies to bovine leukosis virus.

The results of examination of 2 784 sera in the AGIDT-gp were compared with those of the ELISA. A discrepancy was seen in 0.5% of the cases.

Virus-like particles in bovine sera for tissue culture.

Virus-like particles were found in nine different bovine sera for tissue culture from commercial suppliers. These particles were spherical with an overall diam. between 70 and 95 nm.

[Enzootic bovine leukaemia, diagnosis, distribution and control in the Netherlands (author's transl)].

The results of studies on the incidence of enzootic bovine leukaemia in the Netherlands from 1968 to 1975 inclusive are given. The serological methods used (fluorescent antibody technique, immunoperoxidase test, micro complement fixation test and gel diffusion test) were found to be of great value especially for the early diagnosis of the disease. Antibodies to bovine leukaemia virus are already detected long before the onset of persistent lymphocytosis.