[Duodenal evacuatory motor disorders in patients with a resected stomach].

The motor-evacuation disorders in the duodenum after resection of the stomach represent one of the main causes of unsatisfactory results of the operation. These disorders have distinct clinical manifestations and all of them result from stasis of the duodenum content. The main cause of duodenostasis is a mechanical obstacle.

[Functional and morphological characteristics of the gastric mucosa in peptic ulcer and the selection of the method of surgical treatment].

A linkage study of gastric secretion in complicated peptic ulcer in 445 patients, 192 radiotelemetric studies included, was carried out. Histological and histomorphometrical investigations of the stomach mucous membrane were carried out in 179 patients. Gastric resection was performed upon 306 patients: in 228 out of them the resection involved half of the stomach and was supplemented with selective vagotomy in case of hyperacidic secretion and unchanged mucous membrane.

[Protein composition and synthesis in islands of Langerhans isolated from normal rats and rats with alloxan diabetes].

Protein fractions with dissimilar electrophoretic mobility were identified in Langerhans, islands. In solitory islands, isolated from pancreas of rats with alloxan diabetes as compared with normal rats the protein composition varied distinctly: fractions of "constant" proteins and, specifically, the insulin fraction disappeared completely, total amount of identified fractions was decreased with relative alteration in their mobility. The total protein-synthesizing activity of Langerhans' islands from diabetic animals was 3-4-fold decreased as compared with that of control animals.

[Micro-quantitative determination of alpha1-antitrypsin by unidimensional capillary counter-immunodiffusion].

The immunoprecipitation reaction for the determination of microamounts of alpha1-antitrypsin was conducted in the capillaries filled with a microvolume of a 1% agarose gel (the gel length was 7--8 mm, and the diameter--0.6 mm). Microamounts of the antigen and antiserum were applied to the surface of the opposite ends of the gel.

[Neurospecific protein S-100 in the synaptosomes of rat cerebral cortex].

Neurospecific S-100 protein was revealed by the methods of microelectrophoresis in the 15% polyacrylamide gel with a 0.1% sodium dodecylsulfate and by a highly purified S-100 protein "marker" in the composition of low molecular acidic proteins of the rat brain synaptosomes. The S-100 protein conten constitutes about 15-2o% of the low molecular acidic synaptosomal porteins in the rat brain.

[A study of the protein composition of the zona pellucida of mammalian oocytes and zygotes by a method of microelectrophoresis in polyacrylamide gel].

Z. pellucide from oocytes and zygotes of CBA mice and albino rats were lysed in a microvolume of 1% buffered sodium dodecylsulphate (SDS). The microlysate (about 1,0--2,0-10(-7) g protein) was fractionated by means microdiscelectrophoretic technique, using 10%- and 15%-polyacrylamide gel containing 0.

[Detection of alpha 1-antitrypsin in unfertilized ova of albino rats].

500--700 rat oocytes were solubilized in 0.3--0.6 ml of 1% sodium dodecyl sulphate (SDS) --0.

[Molecular weight characteristics of the proteins of oocytes and of cleavage ova in CBA mice by means of capillary microdisc electrophoresis].

5-20 oocytes or cleaving embryos of CBA mice were dissolves in 0.2-0.5 ml of 1% buffered sodium dodecylsulfate (SDS), and soluble proteins were separated using electrophoresis in capillaries filled with PAA-SDS-gel.

[Determination of protein concentration in cleaving mammalian ova by the capillary spectrophotometry method].

A micromodification of the Lowry's method is described which allows to measure reliably the protein content in 5-20 embryos of white rats and CBA mice. Differences in the protein content in rat embryos at the stages of 2 blastomeres and blastocyst were shown to be statistically unreliable (20.2 +/- 1.

[Constitutive proteins of the oocytes and early embryos of rats].

5 protein fractions were identified and their relative mobility was determined in the rat oocytes and cleaving embryos by means of vertical capillary microdisc-electrophoresis in 7.5% polyacrilamide gel (PAA-gel). The same fractions were identified in the cleaving embryos devoid of zona pellucida.

Detection of lactate dehydrogenase isoenzymes in single mammalian oocytes during cleavage by a micromodivication of disc electrophoresis.

A micromodification of the method of disc-electrophoresis in glass capillary tubes is described. It can be used to study the protein composition and activity of lactate dehydrogenase isoenzymes in single rat and mouse oocytes during clevage.