Enhancement of DNAzymatic activity using iterative maturation.

DNAzyme-based (catalytic nucleic acid) biosensing technology is recognised as a valuable biosensing tool in diagnostic medicine and seen as a cheaper, more stable alternative to antibodies or enzymes. However, like enzyme discovery, no method exists to predict DNAzyme sequences that result in high catalytic activity using computer software (). In this work, iterative maturation and evaluation were applied to a DNAzyme oligodeoxynucleotide (ODN) sequence to elucidate novel synthetic sequences with enhanced DNAzyme activity.

Spatially isolated redox processes enabled by ambipolar charge transport in multi-walled carbon nanotube mats.

This work demonstrates a simple dual-well device which enables spatially isolated solutions to undergo complementary redox reactions. The device functions by the ambipolar transport of charge carriers between two spatially isolated poly(dimethylsiloxane) (PDMS) microwells through an underlying multi-walled carbon nanotube (MWCNT) mat. This MWCNT mat enables charge carriers, produced from the decomposition of an analyte in one solution, to drive a redox reaction in a spatially isolated second colorimetric read-out solution via a potential difference between the wells.

Probing Contact Electrification: A Cohesively Sticky Problem.

Contact electrification and the triboelectric effect are complex processes for mechanical-to-electrical energy conversion, particularly for highly deformable polymers. While generating relatively low power density, contact electrification can occur at the contact-separation interface between nearly any two polymer surfaces. This ubiquitousness of surfaces enables contact electrification to be an important phenomenon to understand energy conversion and harvesting applications.

Oxacillin Coupled G-Quadruplexes as a Novel Biofilm-Specific Antibiotic for Biofilms.

One of the most important traits of pathogenic microbial biofilms is their high tolerance to conventional antimicrobial agents, which is partially due to the presence of metabolically inactive and transiently resistant persister cells. Here, we use guanine-rich DNA structures known as G-quadruplexes (G4s) coupled with the β-lactam antibiotic, oxacillin (OX), and loaded with an iron-containing protoporphyrin IX (hemin), as OXG4/hemin complex biofilm-specific antibiotic agents. By coupling the OX to the G4, to form an OXG4/hemin complex, the diffusion of the OX was facilitated into the biofilm.

Hi-fidelity discrimination of isomiRs using G-quadruplex gatekeepers.

Core microRNA (miRNA) sequences exist as populations of variants called isomiRs made up of different lengths and nucleotide compositions. In particular, the short sequences of miRNA make single-base isomiR mismatches very difficult to be discriminated. Non-specific hybridizations often arise when DNA probe-miRNA target hybridization is the primary, or initial, mode of detection.

Adsorption and Desorption of Single-Stranded DNA from Single-Walled Carbon Nanotubes.

The chemical affinity of single-stranded DNA (ssDNA) to adsorb to the surface of single-walled carbon nanotubes (SWCNTs) is used for SWCNT purification, separation and in bio-devices. Despite the popularity of research on SWCNT-ssDNA conjugates, very little work has studied the removal of adsorbed ssDNA on SWCNTs. This paper reports a comprehensive study of biological, physical and chemical treatments for the removal of ssDNA from SWCNT-ssDNA suspensions.

Single nucleotide polymorphism discrimination with and without an ethidium bromide intercalator.

Single nucleotide polymorphism (SNP) genotyping is an important aspect in understanding genetic variations. Here, we discriminate SNPs using toe-hold mediated displacement reactions. The biological target is an 80 nucleotide long double-stranded-DNA from the mtDNA HV1 region, associated with maternal ancestry.