pcnaDeep: a fast and robust single-cell tracking method using deep-learning mediated cell cycle profiling.

Summary: Computational methods that track single cells and quantify fluorescent biosensors in time-lapse microscopy images have revolutionized our approach in studying the molecular control of cellular decisions. One barrier that limits the adoption of single-cell analysis in biomedical research is the lack of efficient methods to robustly track single cells over cell division events. Here, we developed an application that automatically tracks and assigns mother-daughter relationships of single cells.

AGLR is a novel index for the prognosis of hepatocellular carcinoma patients: a retrospective study.

Background: Most hepatocellular carcinoma (HCC) patients' liver function indexes are abnormal. We aimed to investigate the relationship between (alkaline phosphatase + gamma-glutamyl transpeptidase)/lymphocyte ratio (AGLR) and the progression as well as the prognosis of HCC.

Methods: A total of 495 HCC patients undergoing radical hepatectomy were retrospectively analyzed.

Data mining of the expression and regulatory role of BCAT1 in hepatocellular carcinoma.

Branched chain amino acid transaminase 1 (BCAT1) catalyzes the production of glutamates and branched-chain α-ketoacids from branched chain amino acids, and a normal expression is associated with tumorigenesis. Sequencing data from public databases, including The Cancer Genome Atlas, was used to analyze expression and regulation networks for hepatocellular carcinoma (HCC). Expression and methylation were assessed using UALCAN analysis, and data from multiple datasets concerning the expression level and associated survival rates were further analyzed using HCCDB; interaction networks of biological function were constructed using GeneMANIA.

Prognostic Value of Gamma-Glutamyl Transpeptidase to Lymphocyte Count Ratio in Patients With Single Tumor Size ≤ 5 cm Hepatocellular Carcinoma After Radical Resection.

Prediction of prognosis of hepatocellular carcinoma (HCC) has shown an important role in improving treatment outcomes and preventing disease progression, however, the prognostic indicator of HCC is still lacking. The purpose of this study is to investigate the predictive value of GLR (gamma-glutamyl transpeptidase to lymphocyte count ratio) in single HCC with a tumor size (TS) ≤ 5 cm. A retrospective analysis was performed on 272 patients with TS ≤ 5 cm who underwent radical resection.

Prospect of Circular RNA in Hepatocellular Carcinoma: A Novel Potential Biomarker and Therapeutic Target.

CircRNA, a kind of tissue specific and covalently closed circular non-coding RNA is very abundant in eukaryocyte. Generally, circRNA is generated by back-splicing of protein-coding genes' pre-mRNA. Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in the world.