Burst abdomen. Local synthesis of nucleic acids, glycosaminoglycans, proteins and collagen in wounds.

Background And Aims: Abdominal wound dehiscence and evisceration is a surgical emergency. The general phenomena leading to this complication are for the most part known. However, the local biochemical events of different layers of the abdominal wall in wound dehiscence have not been studied.

Burst abdomen. Local synthesis of nucleic acids, glycosaminoglycans, proteins and collagen in wounds.

Background And Aims: Abdominal wound dehiscence and evisceration is a surgical emergency. The general phenomena leading to this complication are for the most part known. However, the local biochemical events of different layers of the abdominal wall in wound dehiscence have not been studied.

Expression of epidermal growth factor and epidermal growth factor receptor genes in healing colonic anastomoses in rats.

This study uses complementary DNA probes to find out if epidermal growth factor (EGF) and EGF receptor genes are activated in healing colonic anastomoses in rats. 35 rats had a colocolonic anastomosis which was removed for study 1, 2, 3, 5, 7, or 14 days after operation. Six animals without surgery served as controls.

Internal hernias after gastric operations.

From a total of 2,146 oesophagojejunal and gastrojejunal anastomoses done between 1969 and June 1990, 7 patients (0.3%) were operated on for internal hernias. Six had developed their hernias after Billroth II gastrectomy with antecolic gastrojejunostomy and enteroanastomosis, and one patient after retrocolic Roux-en-Y reconstruction.

Fibronectin and collagen gene expression in healing experimental colonic anastomoses.

The temporal and spatial expression of fibronectin and type I and III collagen genes were studied 1-14 days after surgery in the healing rat colonic anastomosis using recombinant deoxyribonucleic acid techniques. Messenger ribonucleic acids (mRNAs) coding for fibronectin and type III collagen synthesis increased from the first day after operation and type I collagen synthesis increased from the second day after operation, as demonstrated by Northern hybridizations. Maximal mRNA production for fibronectin and collagens was seen at 2 and 7 days, respectively, after anastomosis.

Formation of granulation tissue in intra-abdominal viscose cellulose sponges. An experimental study in rats.

Formation of intra-abdominal granulation tissue was studied in 32 rats 2, 4, 7 or 21 days after implantation of viscose cellulose sponges. The concentrations of DNA, RNA-ribose, uronic acids, hexosamines, nitrogen and collagen were then measured in the sponges. The rats' body weight fell by 10-15% in the first week, but was regained within 3 weeks.

Bone cement and wound healing. An experimental study in the rat.

The local effect of methyl methacrylate on wound healing was studied in rats using viscose cellulose sponges. A 7 mm diameter cylindrical section was removed from the sponges, filled with freshly prepared bone cement or left empty in controls. Two sponges were implanted subcutaneously into each rat.

Local energy metabolism in healing colon anastomosis. An enzyme-histochemical study in rats.

Local energy metabolism in healing colonic anastomosis was studied in rats. A one-layer, inverting colo-colostomy was performed and frozen sections from the anastomotic area were enzyme-histochemically stained for key enzymes in energy metabolism. Anaerobic metabolism prevailed in the anastomotic area during the first postoperative week.

Girdlestone operation. An acceptable alternative in the case of unreconstructable hip arthroplasty.

Between 1970 and 1984, 1250 total hip arthroplasties using methylmethacrylate bone cement were performed at the Department of Surgery, University of Turku, Finland. Girdlestone arthroplasty was performed in nine women and one man in ten cases of failed arthroplasty. In general, the results were evaluated as good.

Fibronectin, laminin, and collagen types I, III, IV and V in the healing rat colon anastomosis.

The purpose of this work was to study normal anastomotic healing in the rat colon. The unprepared sigmoid colon was divided and a colo-colostomy performed using a one-layer inverting technique. Frozen sections were taken and studied immunohistologically with specific antibodies to fibronectin, laminin and collagen types I, III, IV and V.

Changes in the connective tissue composition of the submucosal layer of colonic anastomosis. An immunohistologic study in rats.

Submucosal healing of colonic anastomosis was studied in rats. The unprepared sigmoid colon was divided and colo-colostomy performed, using a one-layer inverting technique. Frozen sections were studied immunohistologically with specific antibodies to fibronectin, laminin and collagen types I, III, IV and V.

Rupture of the urinary bladder, a potentially serious condition.

During the years 1969 to 1983, 61 patients with rupture of the urinary bladder were treated at the Department of Surgery, University of Turku, Finland. The etiology of the injury was accident trauma in 48%, iatrogenic trauma in 28%, minor trauma in association with misuse of alcohol in 21% and other causes in 3% of the cases. In the first three groups the most reliable signs for diagnosing rupture were abdominal pain and tenderness, together with macroscopic or microscopic haematuria.

Cellular differentiation and expression of matrix genes in type 1 neurofibromatosis.

The cellular heterogeneity of cutaneous tumors from nine patients with type 1 (von Recklinghausen's) neurofibromatosis was studied using several antigenic markers with special reference to focal heterotopic differentiation and interindividual variation. Furthermore, cells which actively express the genes for type I and III collagens and fibronectin, the major components of the abundant extracellular matrix of neurofibromas, were localized using in situ hybridizations. In eight of nine cases, the S-100 protein positive cells, i.

Development of peritoneal fibrosis occurs under the mesothelial cell layer.

This study was carried out in order to find out which part of the peritoneal wall reacts toward silica and produces peritoneal fibrosis. Colloidal silica was injected into the peritoneal cavity of rats to induce chemical peritonitis and frozen sections of the peritoneal wall were stained with specific antibodies toward type I and III collagens and fibronectin. A massive proliferation of granulation tissue was observed between the submesothelial and muscular layers within 48 hr visualized by prominent fibronectin staining.

Factors contributing to subphrenic abscess.

Between 1969 and 1982 11 primary (without previous surgery) and 38 secondary (postoperative) subphrenic abscesses were treated out of total 20800 operations. The patients had on an average more than 4 risk factors. Causes contributing to the formation of secondary abscesses were a difficult operation in 42%, deficiencies in surgical technique in 26%, operative contamination in 21% and peritonitis in 13% of the cases.

Effect of a hexosylceramide fraction of the hemodialysate Solcoseryl on experimental granulation tissue.

This work was carried out to study the effects of a hexosylceramide fraction (Hex-Cer) of the hemodialysate Solcoseryl on developing granulation tissue in rats. Subcutaneously implanted cylindrical hollow viscose-cellulose sponges were used as an inductive matrix for the growth of granulation tissue. In the control animals, the implants were treated daily by withdrawing 1 ml of wound fluid from the central dead space of the implant and by injecting back 1 ml of physiological saline.

Immediate enteral feeding after abdominal surgery.

Enteral feeding by nasoenteral tube was begun immediately after surgery of the alimentary tract in 120 patients. In 37 cases the feeding was delivered proximally to a fresh anastomosis or enterotomy. Immediate enteral feeding was well tolerated even after major elective surgery of the gastrointestinal tract.

Collagen synthesis in cells cultured from v. Recklinghausen's neurofibromatosis.

Subcutaneous tumors of a patient with v. Recklinghausen's neurofibromatosis contained about 31% collagen calculated on the basis of lipid-free dry weight. Slices of the tumors synthesized collagen at a rate (4.

Collagen synthesis in cultured mesothelial cells. Response to silica.

Mesothelial cells were isolated from the peritoneal surface of rats using trypsinization. The cells were polygonal-shaped and proliferated rapidly forming confluent cultures. Application of colloidal silica to mesothelial cell cultures in doses known to induce peritoneal adhesion disease was injurious to the cells and reduced their synthesis of total proteins and collagen.

Effect of peritonitis on oxygen consumption by various tissues and peritoneal fluid cells.

Oxygen consumption by various tissues and peritoneal fluid cells was investigated in silica-induced adhesion disease and fecal peritonitis in rats. Intraperitoneally injected colloidal silica produced chemical peritonitis with a typical acute inflammatory reaction of peritoneal fluid cells showing marked phagocytosis by macrophages. The quantity of cells in the peritoneal fluid as indicated by the content of DNA achieved its maximum within 2-4 days.

Wound infections in abdominal surgery. A prospective study on 696 operations.

A prospective analysis of wound infections in abdominal surgery was carried out in the Department of Surgery, University of Turku, during two 3-month periods. Excluding vascular and urologic surgery altogether 696 abdominal operations were performed. The overall wound infection rate was 9.