Targeted Degradation of ZBP1 with Covalent PROTACs for Anti-Inflammatory Treatment of Infections.

Z-DNA binding protein 1 (ZBP1) has emerged as a critical pathogen-sensing protein that upon activation, triggers necroptotic signaling cascades, leading to a potent inflammatory response and potentially causing significant tissue damage. However, available drugs specifically developed for the effective inhibition or degradation of ZBP1 is still lacking so far. In this study, we developed a potent covalent recognition-based PROTAC (C-PROTAC) molecule for the degradation of ZBP1.

Ultrasensitive colorimetric immunoassay for aflatoxin B detection in lotus seed powder based on enhanced catalysis of Au@Pt with in situ deposition of PtNPs.

The enzyme-linked immunosorbent assay (ELISA) is a powerful and high-throughput method for detecting aflatoxin B (AFB) in food. However, the susceptibility of native enzymes to environmental factors limits their potential applications. This study introduces the combined use of precious metal nanozymes as alternative catalysts to improve the performance of traditional ELISA.

Development of a Reliable ic-ELISA with a Robust Antimatrix Interference Capability Based on QuEChERS Technology for the Rapid Detection of Zearalenone in Edible and Medical Coix Seeds and Subsequent Risk Assessments.

Indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) is an ideal immunoassay method for large-scale screenings to detect mycotoxin contaminants. However, the matrix effect of complicated samples has always been challenging when performing immunoassays, as it leads to false-positive or negative results. In this study, convenient QuEChERS technology combined with optimizing the dilution solvent was ingeniously used to eliminate interference from the sample matrix to greatly improve the detection accuracy, and reliable ic-ELISAs for the two official tolerance levels of 60 and 500 μg/kg were developed to screen zearalenone (ZEN) in edible and medical coix seeds without any further correction.