RACK1 MARylation regulates translation and stress granules in ovarian cancer cells.

Mono(ADP-ribosyl)ation (MARylation) is emerging as a critical regulator of ribosome function and translation. Herein, we demonstrate that RACK1, an integral component of the ribosome, is MARylated by the mono(ADP-ribosyl) transferase (MART) PARP14 in ovarian cancer cells. MARylation of RACK1 is required for stress granule formation and promotes the colocalization of RACK1 in stress granules with G3BP1, eIF3η, and 40S ribosomal proteins.

Optimizing immunogenicity and product presentation of a SARS-CoV-2 subunit vaccine composition: effects of delivery route, heterologous regimens with self-amplifying RNA vaccines, and lyophilization.

Introduction: Dozens of vaccines have been approved or authorized internationally in response to the ongoing SARS-CoV-2 pandemic, covering a range of modalities and routes of delivery. For example, mucosal delivery of vaccines via the intranasal (i.n.

Comparing Human Performance on Target Localization in Near Infrared and Long Wave Infrared for Cluttered Environments.

In the context of rapid advancements in AI, the accuracies and speeds among various AI models and methods are often compared. However, a basic question is rarely asked: is AI better than humans, and if so, under what conditions? This paper investigates human ability to detect distant landmark targets under cluttered surroundings such as buildings, trees, and clouds in NIR and LWIR images, aiming to facilitate AI object detection performance analysis. Our investigation employs perception tests and a human performance model to analyze object detection capabilities.

Hybrid meta/refractive lens design with an inverse design using physical optics.

Hybrid lenses are created by combining metasurface optics with refractive optics, where refractive elements contribute optical power, while metasurfaces correct optical aberrations. We present an algorithm for optimizing metasurface nanostructures within a hybrid lens, allowing flexible interleaving of metasurface and refractive optics in the optical train. To efficiently optimize metasurface nanostructures, we develop a scalar field, ray-wave hybrid propagation method.

Detection and Localization of Small Moving Objects in the Presence of Sensor and Platform Movement.

In this paper, we address the challenge of detecting small moving targets in dynamic environments characterized by the concurrent movement of both platform and sensor. In such cases, simple image-based frame registration and optical flow analysis cannot be used to detect moving targets. To tackle this, it is necessary to use sensor and platform meta-data in addition to image analysis for temporal and spatial anomaly detection.

Radiometry and contrast-to-noise ratio for continuous-wave and laser range-gated active imaging systems.

Resolution and sensitivity must be considered in the design of an active imaging system. System sensitivity is characterized by the signal-to-noise or contrast-to-noise ratio and is derived through radiometry. We present a tutorial for the radiometry associated with the contrast-to-noise ratio for active continuous-wave and laser range-gated imaging systems, giving a useful metric for determining reflective-band sensor performance against a target and background.

Developing multispecies quorum-sensing modulators based on the Streptococcus mitis competence-stimulating peptide.

Bacteria utilize quorum sensing (QS) to coordinate many group behaviors. As such, QS has attracted significant attention as a potential mean to attenuate bacterial infectivity without introducing selective pressure for resistance development. Streptococcus mitis, a human commensal, acts as a genetic diversity reservoir for Streptococcus pneumoniae, a prevalent human pathogen.

A PARP14/TARG1-Regulated RACK1 MARylation Cycle Drives Stress Granule Dynamics in Ovarian Cancer Cells.

Mono(ADP-ribosyl)ation (MARylation) is emerging as a critical regulator of ribosome function and translation. Herein, we demonstrate that RACK1, an integral component of the ribosome, is MARylated on three acidic residues by the mono(ADP-ribosyl) transferase (MART) PARP14 in ovarian cancer cells. MARylation of RACK1 is required for stress granule formation and promotes the colocalization of RACK1 in stress granules with G3BP1, eIF3η, and 40S ribosomal proteins.

Combination of MCL-1 and BCL-2 inhibitors is a promising approach for a host-directed therapy for tuberculosis.

Tuberculosis (TB) accounts for 1.6 million deaths annually and over 25% of deaths due to antimicrobial resistance. Mycobacterium tuberculosis (M.

Noncanonical Streptococcus sanguinis ComCDE circuitry integrates environmental cues in transformation outcome decision.

Natural competence is the principal driver of streptococcal evolution. While acquisition of new traits could facilitate rapid fitness improvement for bacteria, entry into the competent state is a highly orchestrated event, involving an interplay between various pathways. We present a new type of competence-predation coordination mechanism in Streptococcus sanguinis.

A global rise in alluvial mining increases sediment load in tropical rivers.

Increasing gold and mineral mining activity in rivers across the global tropics has degraded ecosystems and threatened human health. Such river mineral mining involves intensive excavation and sediment processing in river corridors, altering river form and releasing excess sediment downstream. Increased suspended sediment loads can reduce water clarity and cause siltation to levels that may result in disease and mortality in fish, poor water quality and damage to human infrastructure.

Broadband metasurface aberration correctors for hybrid meta/refractive MWIR lenses.

A method for designing multi-metasurface layouts for optical aberration correction is presented. All-dielectric metasurfaces are combined with conventional refractive optics to form a hybrid lens. The optical power of a hybrid lens is primarily provided by refractive optics, and metasurfaces are optimized to control optical aberrations.

Investigating the competence regulon through a combination of transcriptome analysis and phenotypic evaluation.

is a recently identified member of the Mitis group of streptococci. This species has been associated with infective endocarditis; however its mechanisms of pathogenesis and virulence are not fully understood. This study aimed to investigate the influence of the competence-stimulating peptide (CSP) and the competence regulon quorum-sensing circuitry (ComABCDE) on subsequent gene transcription and expression, as well as resultant phenotypes.

Broadband metasurface aberration correctors for hybrid meta/refractive MWIR lenses: publisher's note.

This publisher's note contains corrections to [Opt. Express30, 28438 (2022)10.1364/OE.

Rapid changes to global river suspended sediment flux by humans.

Rivers support indispensable ecological functions and human health and infrastructure. Yet limited river sampling hinders our understanding of consequential changes to river systems. Satellite-based estimates of suspended sediment concentration and flux for 414 major rivers reveal widespread global change that is directly attributable to human activity in the past half-century.

Sorption Behavior and Aerosol-Particulate Transitions of Be, Be, and Pb: A Basis for Fallout Radionuclide Chronometry.

We investigated the partitioning of Be, Be, and Pb aerosols between operationally dissolved and >0.5 μm particulate fractions in wet and dry atmospheric deposition. Bulk deposition -log() averaged 4.

Detection of Burmese pythons in the near-infrared versus visible band.

Human task performance studies are commonly used for detecting and identifying potential military threats. In this work, these principles are applied to detection of an environmental threat: the invasive Burmese python. A qualitative detection of Burmese pythons with a visible light camera and an 850 nm near-infrared (NIR) camera was performed in natural Florida backgrounds.

Genetic Context Significantly Influences the Maintenance and Evolution of Degenerate Pathways.

Understanding the evolution of novel physiological traits is highly relevant for expanding the characterization and manipulation of biological systems. Acquisition of new traits can be achieved through horizontal gene transfer (HGT). Here, we investigate drivers that promote or deter the maintenance of HGT-driven degeneracy, occurring when processes accomplish identical functions through nonidentical components.

Volumetric imaging efficiency: the fundamental limit to compactness of imaging systems.

A new metric for imaging systems, the volumetric imaging efficiency (VIE), is introduced. It compares the compactness and capacity of an imager against fundamental limits imposed by diffraction. Two models are proposed for this fundamental limit based on an idealized thin-lens and the optical volume required to form diffraction-limited images.

Spatially coherent regional changes in seasonal extreme streamflow events in the United States and Canada since 1950.

Complex hydroclimate in the United States and Canada has limited identification of possible ongoing changes in streamflow. We address this challenge by classifying 541 stations in the United States and Canada into 15 "hydro-regions," each with similar seasonal streamflow characteristics. Analysis of seasonal streamflow records at these stations from 1910 to present indicates regionally coherent changes in the frequency of extreme high- and low-flow events.

Nanoaperture fabrication in ultra-smooth single-grain gold films with helium ion beam lithography.

We demonstrate a simple three-step gold thin-film sample preparation process to enhance the morphology and lithographic precision using helium ion based direct-writing. The procedure includes metal deposition, heat treatment and template stripping, which produce smooth monocrystalline gold grains with sizes up to 500 nm and an average surface roughness of 0.267 nm.

Imaging-based beam steering for free-space optical communication.

Imaging-based beam steering (IBBS) provides a route to compact, low-power, and low-cost beam steering ideal for mobile free-space optical communications (FSOC), LIDAR imaging, and other active illumination applications. In IBBS, a lens passively maps each pixel of a 2D emitter array into a unique line-of-sight direction. Electronic beam steering of multiple beams with dynamic beam shaping is implemented via pixel selection.