[Chromosome abnormalities and Y chromosome microdeletions in patients with the azoospermia and cryptozoospermia].

Objective: To study the incidence of the chromosome abnormalities and Y chromosome microdeletions in Chinese patients with azoospermia and cryptozoospermia.

Methods: Conventional chromosomal karyotyping was used to analyze the chromosome abnormalities. Genomic DNA was extracted from peripheral blood samples and multiplex polymerase chain reactions (PCR) analyses were performed using specific primers to confirm the presence or absence of Y chromosome microdeletions.

[Relationship between testis volume and types of spermatogenic cells from testicular biopsy in patients with azoospermia or cryptozoospermia].

Objective: To study the relationship between the testis volume and types of spermatogenic cells derived from testicular biopsy in patients with azoospermia or cryptozoospermia.

Methods: We collected testicular pathological biopsies from 492 infertile patients with azoospermia or cryptozoospermia reported in our hospital, classified them according to the testicular histological classification methods in WHO Manual for Standardized Investigation, Diagnosis and Management of the Infertile Male, and analyzed the relationship of the testis volume with the results of semen analyses and testicular histology.

Results: Of the 492 cases, 90.

[Correlation between biological characteristics of volunteer donors and sperm parameters].

Objective: To explore the correlation between the biological characteristics of volunteer donors and sperm parameters.

Methods: Sperm parameters were analyzed for 778 primary volunteer donors by CASA system with standard methods recommended by WHO including sperm concentration, motility rate, VCL, VSL, ALH, LIN, STR and BCF, and their biological characteristics were recorded.

Results: Of all the volunteer donors, students accounted for more than a third, 89.

[Quality analysis of the primary semen samples from 512 donors].

Objective: To evaluate the semen quality of the donors.

Methods: The semen parameters of the primary samples of 512 donors were examined following the World Health Organization (WHO) guide and computer-aided sperm analysis (CASA, product by Harmilton Thorne) system.

Results: Of the 246 (246/512, 48%) donors with potential fertility, only 146 (146/512, 28.

[Comparison of sperm motion parameters in pre-freeze and post-thaw semen samples using computer-assisted sperm analysis].

Objective: To observe sperm motion parameters in pre-freeze and post-thaw semen samples using computer-assisted sperm analysis (CASA) system.

Methods: Semen analyses of 238 samples before freezing and after thawing were separately performed by Hamilton-Thorne Sperm Analyzer.

Results: Sperm motility in post-thaw samples was significantly decreased.