An exopolysaccharide-selenium nanoparticles (EPS-SeNPs) was successfully synthesized by conjugating with Weissella confusa EPS through the reduction of SeO. The EPS-SeNPs composite was comprehensively characterized. These analyses confirmed that the EPS-SeNPs composite had an amorphous nature and a uniform size distribution of around 100 nm.
View Article and Find Full Text PDFLactic acid bacteria (LAB) are well-known for its expertise in synthesizing exopolysaccharides (EPS), which are linked to significant health benefits, such as its prebiotic effects and ability to modulate the immune system. However, the synthesis of EPS is hindered by low yields. The objective of this study was to investigate the impact of co-cultivation on EPS output by Weissella confusa XG-3 when paired with Candida shehatae.
View Article and Find Full Text PDFBacterial cellulose (BC) is a natural, renewable polymer material with an ultrafine nanonetwork structure. However, BC has limited applications in food packaging and medical materials because of its lack of antibacterial properties. To expand the applications of BC, a new BC composite membrane was synthesized via an ex situ method.
View Article and Find Full Text PDFThe interest in nanocomposite films incorporating edible ingredients and active nanoparticles has surged due to their potential to enhance food quality and prolong shelf-life. This research focused on developing innovative exopolysaccharides (EPS)/potato starch (PS) nanocomposite films integrated with g-CN and AgNO. Extensive analysis was conducted to assess the microstructure, physical attributes and antimicrobial properties of these films.
View Article and Find Full Text PDFMultifunctional food packaging composite films were prepared using Pediococcus acidilactici J1 exopolysaccharide (EPS), potato starch (PS) and TiO nanoparticles by casting method. The microstructure, physicochemical properties and antibacterial activity of EPS/PS composite films with different weight ratio of TiO nanoparticles were characterized. Transmission electron microscope (TEM), scanning electron microscopy (SEM) and atomic force microscopy (AFM) revealed the uniform distribution of TiO nanoparticles in the EPS/PS matrix.
View Article and Find Full Text PDFAn exopolysaccharide (EPS)-producing strain, identified as HDL-4, was isolated from litchi. After separation and purification, the structure and properties of HDL-4 EPS were characterized. The molecular weight of HDL-4 EPS was determined to be 1.
View Article and Find Full Text PDFA strain of HDE-8 was isolated from homemade longan fermentation broth. The exopolysaccharide (EPS) yield of the strain was 25.1 g/L.
View Article and Find Full Text PDFBacterial cellulose (BC), as a natural renewable polymer material, has the advantages of porous nanonetwork structure, high degree of polymerization, high purity, high crystallinity, excellent mechanical properties and biocompatibility. However, BC lacks antibacterial properties, which leads to the limitation of BC material in food packaging and medical materials. In this study, a new antibacterial material using the combination of montmorillonite (MMT), BC and exopolysaccharides (EPS) produced by Weissella confusa H2 was synthesized.
View Article and Find Full Text PDFIn this study, an exopolysaccharide (EPS)-producing strain of HDC-01 was isolated from sauerkraut, and the structure, properties and biological activity of the studied EPS were assessed. The molecular weight of the isolated EPS is 2.505 × 10 Da.
View Article and Find Full Text PDFTo explore the effect of Lonicera caerulea fruit polyphenols (LCP) on caries-causing bacteria, strain RYX-01 with high production of biofilm and exopolysaccharides (EPS) was isolated from the oral cavity of caries patients and was identified as Lactobacillus rhamnosus by 16S rDNA analysis and morphology. The characteristics of EPS produced by RYX-01 (EPS-CK) and those produced by adding L. caerulea fruit polyphenols (EPS-LCP) were compared to reveal whether LCP reduced the cariogenicity of RYX-01 by influencing the structure and composition of EPS.
View Article and Find Full Text PDFGlucansucrase was purified from . The glucansucrase exhibited maximum activity at pH 5.5 and 30 °C.
View Article and Find Full Text PDFThe bacterial strain HDE-9 was isolated from sauerkraut and identified as Levilactobacillus brevis. An exopolysaccharide (EPS) was isolated and purified from L. brevis HDE-9, and a preliminary investigation of its structural characteristics and biological activity was conducted.
View Article and Find Full Text PDFIn this study, the exopolysaccharide (EPS) from Saccharomyces cerevisiae Y3 was extracted and purified, the preliminary structure characteristics and biological activities was investigated. The S. cerevisiae Y3 EPS was obtained by ethanol precipitation and gel filtration chromatography.
View Article and Find Full Text PDFInt J Biol Macromol
April 2022
An EPS produced by Weissella confusa H2 was purified through Sephadex G-100, and the preliminary structure characteristics and biological activities of H2 EPS were analyzed. Molecular mass of purified H2 EPS was 2.705 × 10 Da as measured with gel permeation chromatography (GPC).
View Article and Find Full Text PDFIn this study, the effects of penicillin G (PENG) on the fate of bacterial communities and β-lactamase antibiotic resistance genes (ARGs) during chicken manure composting were assessed, to illustrate the roles of PENG in ARGs behavior. The results showed that the total absolute abundances of 9 ARGs and 4 mobile genetic elements (MGEs) was significantly increased by PENG (P < 0.05).
View Article and Find Full Text PDFA new strain of bacterial cellulose (BC)-producing Gluconobacter cerinus HDX-1 was isolated and identified, and a simple, low-cost complexation method was used to biosynthesis Lactobacillus paracasei 1∙7 bacteriocin BC (BC-B) nanofiber. The structure and antibacterial properties of the nanofibers were evaluated. Solid-state nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) analysis showed that BC and BC-B nanofibers had typical crystalline form of the cellulose I.
View Article and Find Full Text PDFPrep Biochem Biotechnol
August 2022
A lactic acid bacteria (LAB) isolated from pickled Chinese cucumber was screened for bacteriocin production. The strain was identified to be HDX-2. Based on the Plackett-Burman (PB) experiment, glucose, Ca, and initial pH were found to be the most significant parameters of bacteriocin production.
View Article and Find Full Text PDFResponse surface methodology (RSM) was used to optimize the conditions of exopolysaccharides (EPSs) by Y3. The results indicated that the yield of EPS reached 4.52 ± 0.
View Article and Find Full Text PDFDRP105 isolated from Chinese sauerkraut juice is an intensive producer of dextran. We report the complete genome sequence of DRP105. This strain contains a dextransucrase gene () involved in the production of dextran, possibly composed of glucose monomers.
View Article and Find Full Text PDFObjective: Although the extracellular polysaccharides have been analyzed in the previous period, the biochemical, enzymological characters and stimulation and inhibition effect on glucansucrase are not fully understood.
Results: After three steps purification, salting out, DEAE-Sepharose and Sephadex G-75, the final specific activity was 264.84 U/mg protein with 4.
Enterococcus faecium TJUQ1 with high bacteriocin-producing ability was isolated from pickled Chinese celery. In this study, enterocin TJUQ1 was purified by ammonium sulfate precipitation, reversed-phase chromatography (Sep-Pak C) and cation-exchange chromatography. The activity of the purified bacteriocin was 44,566.
View Article and Find Full Text PDFTo synthesize biomaterials for antibacterial applications, chitosan (CS) and chitooligosaccharide (COS) were successfully incorporated into bacterial cellulose (BC) matrix respectively. The representative Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) were selected to evaluate the antibacterial effects of the composite membranes. The results manifested BC-CS and BC-COS membranes exhibited remarkable antibacterial properties against both bacteria with the inhibition ratio of 99.
View Article and Find Full Text PDFThe fermentation conditions for exopolysaccharides (EPS) with a highly branched structure extracted from Leuconostoc citreum B-2 were optimized by response surface methodology (RSM). The results showed that under the optimal fermentation conditions of sucrose, yeast extract and pH of 99.80 g/L, 5.
View Article and Find Full Text PDFInt J Biol Macromol
October 2019
An exopolysaccharide (EPS)-producing bacterium was isolated from the homemade fermentation product of hami melon (Cucumis melo var. saccharinus) and identified as Leuconostoc lactis L2 mainly based on 16S rDNA sequence analysis and the API 50 CHL test. The monosaccharide composition of Leu.
View Article and Find Full Text PDFInt J Biol Macromol
September 2019
A glucansucrase encoding gene was cloned into pET-28a(+) vector and expression in Escherichia coli BL21(DE3). An about 160 kDa recombinant glucansucrase was purified with a yield of 50.73% and a 4.
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