Objectives: Autoimmunity, hypersensitivity, and the recently recognized set of syndromes collectively termed immunoglobulin G4-related disease (IgG4-RD) may be associated with increased serum IgG4 levels. We reviewed our experience detecting increased IgG4 by distinct serum protein electrophoresis (SPEP) patterns.
Methods: We studied 303 capillary SPEP cases with dome-like anodal γ changes and increased measured serum IgG4.
We report the precision of the mean migration position of hemoglobin (Hb)S, HbC, HbG (Philadelphia), and HbD (Los Angeles) in 193 samples of whole blood assayed by capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC). By expressing the migration of Hb variants by CE relative to that of HbA(2) in the same sample, there was a significant improvement in the coefficient of variation for each variant studied. The potential usefulness of expressing Hb variants relative to that of HbA(2) was evaluated by comparing the separation of 2 closely migrating Hbs.
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