Molecular surveillance of potential SARS-CoV-2 reservoir hosts in wildlife rehabilitation centers.

Background: The COVID-19 pandemic, caused by SARS-CoV-2 infection, has become the most devastating zoonotic event in recent times, with negative impacts on both human and animal welfare as well as on the global economy. Although SARS-CoV-2 is considered a human virus, it likely emerged from animals, and it can infect both domestic and wild animals. This constitutes a risk for human and animal health including wildlife with evidence of SARS-CoV-2 horizontal transmission back and forth between humans and wild animals.

First molecular detection of Equine Herpesvirus type 3 (EHV-3) in Chile.

Equine coital rash (ECE) is a highly contagious benign infection that induces lesions on external genitals, and it is caused by the equine herpesvirus type 3 (EHV-3). Although the disease is globally distributed, its presence in Chile has not been documented from a genetic point of view. Here, we performed polymerase chain reaction screenings for EHV-3 in lesions of external genitals in four horses belonging to a riding station at Bulnes, Ñuble Region, Chile.

First record of in (Carnivora, Felidae) and (Carnivora, Mustelidae): new hosts in Chile.

Background: Trichinellosis is a zoonotic disease with a worldwide distribution. It is caused by several species of nematodes in the genus spp. are transmitted through predation or carrion consumption and occur in domestic and sylvatic cycles.

Survey of Trichinella in American minks (Neovison vison Schreber, 1777) and wild rodents (Muridae and Cricetidae) in Chile.

Trichinellosis is a zoonosis with worldwide distribution. Several hosts have been recognized around the world; however, there is a lack of knowledge of the role of feral mammals in Chile in its transmission. Herein, we tested muscle samples from 555 individuals among American minks (Neovison vison Schreber, 1777.

Molecular Survey of Parvoviruses and Mycoplasma spp. in Invasive American Mink (Neovison vison) from Southern Chile.

Using PCR, we evaluated the presence of parvoviruses and Mycoplasma spp. in 123 American mink (Neovison vison), an introduced invasive carnivore in Chile. Our results showed all analyzed animals were negative for both pathogen groups.

Domestic dog origin of Carnivore Protoparvovirus 1 infection in a rescued free-ranging guiña (Leopardus guigna) in Chile.

Carnivore protoparvovirus 1 is one of the most important pathogens affecting both wild and domestic carnivores. Here, we reported the genetic characterization of canine parvovirus (CPV-2) strains from a rescued guiña (Leopardus guigna) and domestic dogs from Chile. Guiña strain was classified as CPV-2c, and phylogenetic analysis of the complete coding genome showed that the guiña CPV-2c strain shares a recent common ancestor with Chilean domestic dogs' strains.

First Molecular Identification of Canine Parvovirus Type 2 (CPV2) in Chile Reveals High Occurrence of CPV2c Antigenic Variant.

Canine parvovirus type 2 (CPV2) is one of the most important intestinal pathogens in dogs and puppies. CPV2 has been evolved into three genetic and antigenic variants (2a, 2b, and 2c), which are distributed worldwide. We reported the first study of genetic diversity of CPV2 in Chile.

Morphological and molecular identification with frequency analysis of Calicophoron microbothrioides in central Chile.

This research aimed to determine the presence of paramphistomids in cattle slaughtered in a slaughterhouse of the Ñuble Region of Chile, to identify flukes and to analyze the frequency of these parasites in the Maule, Ñuble, and Biobío administrative regions of Chile. Between October of 2016 and April of 2017, rumens of 494 cattle were examined for flukes in the forestomachs. Worms were identified morphologically and, in addition, molecular analysis of the internal transcriber spacer region 2 of the fluke's DNA was done and phylogenetic analyses were performed with Bayesian inference in 14 worms.

Zoonotic Pathogens in the American Mink in Its Southernmost Distribution.

The American mink, , is an invasive species in Chile. Its impact on native fauna and public health has not been studied in depth in the country. In this study, we searched for gastrointestinal parasites, including helminths and zoonotic sp.

Reemergence of H3N8 Equine Influenza A virus in Chile, 2018.

A new outbreak of equine Influenza A virus (IAV) was reported in Chile in January 2018, 6 years after its last report in 2012. Equine IAV was detected by rtRT-PCR, followed by virus isolation and full genome sequencing. Genetic characterization of equine IAV classified the virus within clade 1 of the Florida sublineage.

Epidemiological investigations of the introduction of porcine reproductive and respiratory syndrome virus in Chile, 2013-2015.

Porcine reproductive and respiratory syndrome (PRRS) is endemic in most pork producing countries. In Chile, eradication of PRRS virus (PRRSV) was successfully achieved in 2009 as a result of the combined efforts of producers and the animal health authorities. In October 2013, after several years without detecting PRRSV under surveillance activities, suspected cases were confirmed on a commercial swine farm.

HEMATOLOGIC AND BIOCHEMICAL PARAMETERS OF KELP GULLS (LARUS DOMINICANUS) CAPTURED IN THE CITY OF TALCAHUANO, CHILE.

In order to provide tools for future health-based monitoring programs, we developed reference intervals for hematology and plasma biochemistry and partitioned data for sex and season (winter and autumn). Ninety-one physically healthy kelp gulls (Larus dominicanus) were sampled in the city of Talcahuano, Chile, during winter (July-September) of 2007, autumn (April-June) and winter of 2008, and autumn of 2009. After blood sampling, the kelp gulls were euthanized by cervical dislocation.

Feline immunodeficiency virus and feline leukemia virus infection in free-ranging guignas (Leopardus guigna) and sympatric domestic cats in human perturbed landscapes on Chiloé Island, Chile.

Feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) are two of the most common viruses affecting domestic cats (Felis catus). During the last two decades, reports show that both viruses also infect or affect other species of the family Felidae. Human landscape perturbation is one of the main causes of emerging diseases in wild animals, facilitating contact and transmission of pathogens between domestic and wild animals.

Evidence for Kelp Gulls (Larus dominicanus) and Franklin's Gulls (Leucophaeus pipixcan) as carriers of Salmonella by real-time polymerase chain reaction.

Polymerase chain reaction confirmed that two gull species in Talcahuano, Chile, shed Salmonella. Fecal samples from resident Larus dominicanus had prevalences of 51.2% for Salmonella spp.