Construction of a one-step multiplex real-time PCR assay for the detection of serogroups A, B, and E of associated with bovine pasteurellosis.

Bovine pasteurellosis, caused by serogroups A, B, and E of (Pm), is mainly manifested as bovine respiratory disease (BRD) and hemorrhagic septicemia (HS). The disease has caused a great economic loss for the cattle industry globally. Therefore, identifying the Pm serogroups is critical for optimal diagnosis and subsequent clinical treatment and even epidemiological studies.