Publications by authors named "Rencai Feng"

Article Synopsis
  • - The study analyzed the enzyme activity and metabolites of mycelium from a wild edible fungus over different growth periods, noting that enzyme activity generally peaked around day 20, except for specific cases like laccase on day 10 and extracellular amylase on day 15.
  • - A total of 3569 metabolites were detected, with terpenoids being the most abundant; the research highlighted different accumulation patterns of metabolites at various stages (10, 20, and 30 days) related to growth and development.
  • - The findings suggest that a 20-day cultivation cycle is optimal for liquid spawn production, and emphasize the significant role of amino acid metabolism in mycelium growth and development.
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To fully utilize Phellinus igniarius fermentation mycelia, the present study investigated the in vitro antioxidant and α-amylase inhibitory properties of four Ph. igniarius strains. Organic solvents were used to extract fatty acids, phenolics, and flavonoids from the selected mushrooms.

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This study focuses on optimizing the mutagenesis process for (Mel-7) mycelia through atmospheric and room temperature plasma (ARTP) mutation and explores the resultant thermal adaptability and physiological responses of mutant strains. This research demonstrated a clear relationship between ARTP mutagenesis exposure duration and lethality rate, indicating that an exposure time of 40 s resulted in the optimal balance of inducing mutations without causing excessive mortality. Additionally, this study established 43 °C as the ideal screening temperature for identifying mutant strains with enhanced heat resistance, as this temperature significantly challenges the mycelia while allowing thermotolerant strains to be distinguishable.

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Floccularia luteovirens is one of the rare edible fungi with high nutritional value found on the Qinghai-Tibet Plateau. However, research at the molecular level on this species is currently constrained due to the lack of reliable reference genes for this species. Thirteen potential reference genes (ACT, GAPDH, EF-Tu, SAMDC, UBI, CLN1, β-TUB, γ-TUB, GTP, H3, UBC, UBC-E2, and GTPBP1) were chosen for the present study, and their expression under various abiotic conditions was investigated.

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Hymenopellis radicata (H. radicata) is an edible fungus rich in protein and mineral elements, with high edible and medical value. And reference genes suitable for normalization of qRT-PCR data from this species have not been investigated.

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Through whole-genome re-sequencing of 18 germplasm resources collected from diverse regions in China, we identified significant variations in the form of Single Nucleotide Polymorphisms (SNPs) and Insertions and Deletions (InDels). These variations were comprehensively annotated, shedding light on the mutation types present in the entire genome of the germplasm. This analysis revealed the number and position information of each mutation and provided insights into the overall genomic landscape of germplasm.

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spp. have historically been used as traditional medicines to treat various diseases owing to their antioxidant, antitumor, and antidiabetic activities. Polysaccharides exhibit antidiabetic activity.

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, a rare wild edible and medicinal fungus, is endemic to the Tibetan plateau. However, attempts to artificially domesticate this species have not been successful, resulting in extremely limited utilization of this valuable resource. This paper presents the geographical distribution of along with its ecological and biological characteristics.

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Atmospheric and room-temperature plasma (ARTP) is an efficient microbial mutagenesis method with broad application prospects. Compared to traditional methods, ARTP technology can more effectively induce DNA damage and generate stable mutant strains. It is characterized by its simplicity, cost-effectiveness, and avoidance of hazardous chemicals, presenting a vast potential for application.

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Mushrooms are full of nutrition and have beneficial properties for human health. Polysaccharides are the main component of edible and medicinal mushrooms, especially β-glucans, which have attracted much more attention for their complex structure and diverse biological activities. Among all the diverse medicinal activities of mushroom polysaccharides, antitumor and immune-enhancing activities are two excellent bioactivities that have much more potential and deserve application.

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Article Synopsis
  • Le023M is a mutant strain of a prized edible mushroom in China that shows remarkable ability to withstand high temperatures, recovering faster from heat stress compared to its parent strain, Le023.
  • The research involving RNA-seq and qRT-PCR indicates that Le023M expresses several heat shock proteins and accumulates specific metabolites like trehalose, aspartate, and glutamate, which are essential for its thermal resilience.
  • These insights into the molecular mechanisms of thermotolerance in Le023M could inform strategies for cultivating this mushroom under challenging high-temperature conditions.
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(), one of the most popular edible mushrooms in China, is adversely affected by high temperature. Heat shock proteins (HSPs) play a crucial role in regulating the defense responses against the abiotic stresses in . Some HSPs in have been described previously, but a genome-wide analysis of these proteins is still lacking.

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Edible fungus is a large fungus distributed all over the world and used as food and medicine. But people's understanding of edible fungi is not as much as that of ordinary crops, so people have started a number of research on edible fungi in recent years. With the development of science and technology, omics technology has gradually walked into people's vision.

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Article Synopsis
  • Edible and medicinal mushrooms are highly nutritious and offer a variety of health benefits due to their rich bioactive compounds, making them valuable environmental crops.
  • The review focuses on various breeding techniques for these mushrooms, comparing traditional mutagenesis methods with modern gene-editing techniques like CRISPR/Cas9, highlighting their respective impacts on strain development.
  • Future strategies for mushroom breeding will integrate genome sequencing, metabolic engineering, and advanced mutagenesis methods to create improved strains with enhanced quality and yield.
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Phellinus igniarius is a medicinal fungus possessing potent therapeutic activity due to the polysaccharides, polyphenols, flavonoids, and other secondary metabolites they contain. Laccases are crucial enzymes involved in lignin degradation in Ph. igniarius and offer great potential to accomplish several bioprocesses.

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