A scalable suspension insect cell transfection method for production of baculoviruses with low amplification passages.

Baculovirus expression vector systems (BEVS) have been widely used for production of recombinant proteins in insect cells. However, baculoviruses superinfection and repeated passages originate defective interfering particle (DIP) mutants, which is a limitation to a continuous large-scale production. Accordingly, a classical chemical transfection method performed on monolayer of insect cells (Sf9) was modified to produce recombinant baculoviruses with high efficiency.

Dataset on recombinant expression of an ancient chitinase gene from different species of parasites in bacteria and in cells using baculovirus.

The data presented here is related to negative results obtained with the recombinant expression of chitinase from four species of parasites in two expression systems, performed in order to investigate the molecular characteristics of the chitinase and its possible application in leishmaniasis diagnosis. Thus, heterologous chitinase proteins were expressed in bacteria using the prokaryotic expression vector pET28a and Mach-T1, and in (Sf9) insect cells, using the eukaryotic expression system (Thermo Fisher Scientific) to produce recombinant baculoviruses to infect Sf9. Biochemical and cellular analysis of the various recombinant forms of the chitinase produced in prokaryotic and eukaryotic expression systems were performed through SDS-PAGE and Western blotting.

Targeting mitochondria in melanoma: Interplay between MAPK signaling pathway and mitochondrial dynamics.

Melanoma is a malignant proliferative disease originated in melanocytes, characterized by high metastatic activity and by the activation of oncogenes, such as B-RAF (40-60% of cases). Recent studies have shown that vemurafenib (a MAPK inhibitor) promoted disturbance of mitochondrial bioenergetics, although underlying mechanisms are not fully comprehended. Here we showed that MAPK inhibition by vemurafenib in B-RAF-mutated human melanoma culminated in the inhibition of DRP1 phosphorylation, associated to a large mitochondrial network remodeling to the hyperfused phenotype, and increased oxidative phosphorylation capacity.

Co-circulating serotypes in a dengue fever outbreak: Differential hematological profiles and phylogenetic relationships among viruses.

Background: Dengue virus, represented by four distinct, genetically diverse serotypes, is the etiologic agent of asymptomatic to severe hemorrhagic diseases. The spatiotemporal dynamics of dengue serotypes and its association to specific diseases vary among the different regions worldwide. By 2007, and in São Paulo State, Brazil, dengue-case concentration in urban centers had changed to increased incidence in small- and medium-sized towns, the case of Marília.