Bioconjugation of peptides using advanced nanomaterials to examine their interactions in 3D printed flow-through device.

Peptide-peptide interactions are crucial in the living cell as they lead to the formation of the numerous types of complexes. In this study, synthetic peptides containing 11 of cysteines (α-domain of metallothionein (MT)) and sialic acid binding region (130-loop of hemagglutinin (HA)) were employed. The aim of the experiment was studying the interactions between MT and HA-derived peptides.

Use of nucleic acids anchor system to reveal apoferritin modification by cadmium telluride nanoparticles.

The aim of this study was to synthesize cadmium telluride nanoparticles (CdTe NPs) modified apoferritin, and examine if apoferritin is able to accommodate CdTe NPs. Primarily, the thermostability of horse spleen apoferritin was tested and it's unfolding at 70 °C was observed. Cadmium telluride nanoparticles (CdTe NPs) were synthesized both within apoferritin protein cage and on its surface.

Interaction study of arsenic (III and V) ions with metallothionein gene (MT2A) fragment.

Arsenic compounds belong to the most controversial agents concerning human health. Arsenic (As) is considered as a top environmental element influencing human health due to its adverse effects including cancer, diabetes, cardiovascular disease, and reproductive or developmental problems. Despite the proven mutagenic, teratogenic and carcinogenic effects, the arsenic compounds are used for centuries to treat infectious diseases.

Lead ions encapsulated in liposomes and their effect on Staphylococcus aureus.

The aim of the study was the preparation of a liposome complex with encapsulated lead ions, which were electrochemically detected. In particular, experiments were focused on the potential of using an electrochemical method for the determination of free and liposome-encapsulated lead and determination of the encapsulation efficiency preventing the lead toxicity. Primarily, encapsulation of lead ions in liposomes and confirmation of successful encapsulation by electrochemical methods was done.

Distribution of mercury in rainbow trout tissues at embryo-larval and juvenile stages.

The aims of the study were to determine total mercury concentrations in "rainbow trout Oncorhynchus mykiss (Walbaum)" at their embryo-larval and juvenile stages and to assess mercury concentration dynamics in individual tissues. Samples of rainbow trout were collected at two-month intervals over a period of 18 months (one stock production cycle) at the Velká Losenice trout farm. Feedstuff samples were collected at the same time and analyzed for mercury concentrations.

Using human hair as an indicator for exposure to mercury.

Objectives: Exposure to mercury, a risk factor for neuro-developmental toxicity, was evaluated in the Czech Republic by performing mercury determination using human hair as an indicator.

Methods: Hair samples from Czechs (n=311; 2-66 years old) were analyzed for mercury content. Total mercury was analysed by mercury analyzer AMA 254.

Mercury in human hair as an indicator of the fish consumption.

Objectives: Mercury and most of its compounds are extremely toxic and should be handled with care. It can be inhaled and absorbed through the skin and mucous membranes. The most toxic forms of mercury are its organic compounds such as dimethylmercury and methylmercury.