Publications by authors named "Renata K N R Silva"
Background: Microscopy and enzyme-linked immunosorbent assay (ELISA) are routinely used for Cryptosporidium diagnosis, without differentiating the parasite species.
Methods: Children's feces were analyzed by modified Ziehl-Neelsen (mZN) and ELISA for Cryptosporidium diagnosis and by polymerase chain reaction-restriction fragment length polymorphism for species identification.
Results: Cryptosporidium frequency was 2.
Techniques for Giardia diagnosis based on microscopy are usually applied as routine laboratory testing; however, they typically exhibit low sensitivity. This study aimed to evaluate Giardia duodenalis and other intestinal parasitic infections in different pediatric groups, with an emphasis on the comparison of Giardia diagnostic techniques. Feces from 824 children from different groups (diarrheic, malnourished, with cancer and from day care) were examined by microscopy and ELISA for Giardia, Cryptosporidium sp.
View Article and Find Full Text PDFDifferences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory.
J Parasitol
December 2013
Article Synopsis
- Despite various methods available for detecting Cryptosporidium and Isospora belli in fecal samples, there are concerns about their accuracy in practice.
- The study analyzed 27 positive stool samples for Cryptosporidium and 15 for I. belli using two concentration methods and three staining techniques, finding no major differences in detecting Cryptosporidium but significantly better detection of I. belli using centrifugation.
- The results highlight that different methods have specific strengths and weaknesses, and the detection of these intestinal parasites can vary significantly depending on the concentration and staining techniques used.