Biological effects of ionizing radiation.

Ionizing radiation refers to the flow of material particles or photons of electromagnetic radiation that can ionize atoms of the environment through which it passes. At the cellular level, damage can be manifested by cell death or changing their cytogenetic information. These events can lead to adverse tissue reactions (deterministic effects) in which manifestations depend on exceeding the dose threshold, or to the stochastic effects, when the probability of damage increases with the dose.

Radioactivity in mushrooms from selected locations in the Bohemian Forest, Czech Republic.

Cs is one of the most important radionuclides released in the course of atmospheric nuclear weapon tests and during accidents in nuclear power plants such as that in Chernobyl, Ukraine, or Fukushima, Japan. The aim of this study was to compare Cs and K concentrations in particular species of mushrooms from selected locations in the Bohemian Forest (Czech: Šumava), Czech Republic, where a considerable contamination from the Chernobyl accident had been measured in 1986. Samples were collected between June and October 2014.

Mapping the factors affecting the frequency and types of micronuclei in an elderly population from Southern Bohemia.

The micronucleus assay is one of the most common methods used to assess chromosomal damage (losses or breaks) in human peripheral blood lymphocytes (PBL) in genetic toxicology. Most studies have focused on analyzing total micronuclei (MN), but identifying the content of MN can provide more detailed information. The main aim of this study was to map the factors affecting the frequency and types of micronuclei in binucleated cells (BNC) in elderly population.

Effective Doses of Employees at the Former Uranium Processing Plant MAPE Mydlovary, Czechoslovakia.

Results are presented of a survey of almost 1000 dosimetric records of employees at the former uranium processing plant MAPE Mydlovary. Located ~20 km to the north-west of České Budějovice in South Bohemia, it was the place where most of the uranium ore mined in Czechoslovakia in the years from 1962 to 1991 was processed. The records refer to incorporation of short-lived radon progeny and long-lived radionuclides as well as external gamma exposure.

Analysis of Genetic Damage in Lymphocytes of Former Uranium Processing Workers.

The frequency of cells containing micronuclei (MN) and the presence of centromeres in these MN were analyzed in lymphocytes of 98 men from Southern Bohemia. Forty-six of them had worked at the uranium processing plant 'MAPE Mydlovary' which was closed in 1991, and 52 men were controls from the same area. FISH using human pan-centromeric chromosome paint was employed to detect centromere-positive (CEN+) and -negative (CEN-) MN.

Micronuclei in lymphocytes from radon spa personnel in the Czech Republic.

Purpose: To assess the radiation exposure of radon spa personnel in Jáchymov, Czech Republic.

Methods: The frequency of micronucleus-containing cells and the percentage of centromere-free micronuclei (micronuclei containing only acentric fragments) was determined in peripheral blood lymphocytes of 42 individuals working at the Jáchymov spa and 42 control individuals.

Results: There was a highly significant increase in the frequency of micronucleus-containing cells as well as the percentage of centromere-free micronuclei in the lymphocytes of spa personnel versus controls.

Micronuclei in lymphocytes from currently active uranium miners.

Micronuclei can be used as markers of past radiation exposure, but only few studies have dealt with uranium miners. In this paper, we report on micronuclei in lymphocytes from individuals currently working at Rožná, Czech Republic, the last functioning uranium mine in the European Union. A modified micronucleus-centromere test was applied to assess the occurrence of micronuclei in stimulated lymphocytes, as well as their content in terms of whole chromosomes or fragments.

Radioprotective effects of amifostine (WR-2721) or cystamine on radiation damage and its repair in rats whole body exposed to fission neutrons.

Sulphur containing radioprotective drugs amifostine (gammaphos, WR-2721) or cystamine (disulfide of meracaptoethylamine) of Czechoslovak production were examined in whole body fission neutrons irradiated rats in the thermal column of reactor VVR-S. Using the split-dose technic the first sublethal neutron dose in the range 1-2 Gy was followed by second lethal exposures in the two time intervals (3 or 6 days) using whole body fission neutrons irradiations (3 days interval) or whole body gamma-irradiations (6 days interval) for LD50/30 evaluation within next 30 days survival observation. In other experiments the mean survival time (MST) in days was estimated in different rats group, when animals were whole body fission neutrons irradiated twice with 3-days interval using the total lethal doses of 4 or 5 Gy.