Molecular detection of Chlamydiales organisms in ruminant abortions in Brazil.

Infections caused by the Chlamydiales order are highly prevalent worldwide and have a well-known association with reproductive and non-reproductive disorders in livestock and humans. This study aimed to identify Chlamydiales organisms in ruminant abortions using real-time polymerase chain reaction (qPCR) in samples collected from three Brazilian states: Rio Grande do Sul, Santa Catarina, and São Paulo. The Chlamydiales order was found in 7.

Immunocytochemistry of bone marrow aspirates: a tool in the diagnosis of feline leukemia virus infection in cats.

Feline leukemia virus (FeLV) is a highly debilitating cat pathogen due to its ability to cause many pathological changes. Therefore, identifying the virus directly in bone marrow can be a highly relevant diagnostic tool even in the absence of viraemia. The aim of this study was to compare the diagnostic efficiency of immunocytochemistry (ICC) of bone marrow aspirates with enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR).

Neoplastic and non-neoplastic diseases associated with feline leukaemia virus (FeLV) in cats in southern Brazil.

The objective of this study was to categorise diseases associated with FeLV infection in cats. A total of 154 cats were submitted to necropsy, histopathology exam and anti-FeLV immunohistochemistry (IHC), and 83 (50.9 %) were IHC FeLV-positive.

IN PSITTACINES KEPT IN CAPTIVITY IN THE STATE OF SANTA CATARINA, BRAZIL.

are responsible for proventricular dilatation disease (PDD) in psittacines. This study aimed to determine the occurrence and factors associated with infection in psittacines kept in captivity in a state in the southern region of Brazil. A cross-sectional study was carried out with 192 birds from two facilities (A and B) in 2019, using choanal, esophageal, and cloacal swabs and feathers, totaling 768 samples subjected to reverse-transcription polymerase chain reaction (RT-PCR), for the matrix (M) protein gene with a final product of 350 base pairs (bp).

Sheep abortions associated with Neospora caninum and Toxoplasma gondii infections in multiple flocks from Southern Brazil.

Neosporosis and toxoplasmosis are important parasitic causes of abortions in small ruminants. This study verified the occurrence of these diseases in sheep fetuses from Santa Catarina State, Southern Brazil from 2015 to 2022. Sheep fetuses were necropsied with organ sampling for histopathology, and polymerase chain reaction (PCR) for Neospora caninum and Toxoplasma gondii using the Nc5 and SAG2 targets, respectively, in frozen brain tissue.

Toxoplasmosis outbreak caused by north American genotypes in captive black-tufted marmosets in southern Brazil.

In this study, we describe an outbreak of toxoplasmosis in an enclosure of five Callithrix penicillata in Southern Brazil. The marmosets were found dead and submitted to necropsy and histopathology. Liver, spleen, and lungs were frozen and sent for polymerase chain reaction (PCR) and nested-PCR-restriction fragment length polymorphism (RFLP) genotyping of Toxoplasma gondii.

Uncommon bovine viral diarrhea virus subtype 1e associated with abortions in cattle in southern Brazil.

We characterized bovine viral diarrhea virus (BVDV)-related abortions in cattle and identified the species and subgenotypes in the state of Santa Catarina, southern Brazil. Our RT-PCR assay was positive for BVDV in 5 fetuses from different farms; however, 3 of the 5 fetuses were also PCR-positive for . In the 5 BVDV-positive fetuses, gross lesions included fetal mummification (1), hepatomegaly (1), subcutaneous edema (1), and perirenal edema (1).

Overview of Bone Marrow Aspiration from 120 Cats in Different Hematological Conditions.

Bone marrow (BM) evaluation is highly important for the diagnosis of numerous hematological alterations in animals, especially cats, given their greater propensity for hematopoietic changes associated with retrovirus infections. This study aims to describe the main aspects of the BM of cats with different hematological conditions, comparing with reference intervals established from animals without hematological alterations and also with a previously established reference interval. To do so, we evaluated full blood and BM samples from 120 cats with no abnormalities on physical examination, negative for FeLV and FIV.

Causes of fetal death in the Flemish cattle herd in Brazil.

Background And Aim: Flemish cattle in Brazil are on the brink of extinction and are found only in one herd in Lages, Santa Catarina State. This study aimed to uncover the reasons for the recurring abortions in the Flemish cattle herd.

Materials And Methods: Seventeen Flemish fetuses underwent postmortem examinations, with samples collected for histopathology and microbiology culture tests, polymerase chain reaction (PCR) test for , and reverse transcription-PCR (RT-PCR) test for bovine viral diarrhea virus (BVDV) from 2015 to 2020.

Mycoplasma haemofelis infection and its correlation with feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) in cats in Southern Brazil.

A cross-sectional study was conducted on 274 cats in Southern Brazil to estimate the prevalence of Mycoplasma haemofelis PCR, associated factors, and its correlation with ELISA for FeLV and FIV. The apparent prevalence of M. haemofelis was 6.

Neospora caninum-associated abortions in cattle from Southern Brazil: Anatomopathological and molecular characterization.

This study aimed to evaluate the N. caninum associated abortions in cattle in the state of Santa Catarina, in the southern Brazil. Aborted bovine fetuses were necropsied, submitting organ samples for histopathological evaluation.

Microcephaly and hydrocephalus in a sheep fetus infected with Neospora caninum in Southern Brazil - Short communication.

A case of non-communicating hydrocephalus and microcephaly in a sheep fetus infected with Neospora caninum from Lages, Santa Catarina, Brazil, is reported. Macroscopically, there was moderate flattening and narrowing of the skull, and the portion of the cerebral hemispheres was markedly reduced in size, measuring 3.5 × 3.

Canine Leproid Granuloma (CLG) Caused by Mycobacterial Species Closely Related to Members of Mycobacterium Simiae Complex in a Dog in Brazil.

This report describes the clinical features and molecular diagnosis of a case of canine leproid granuloma (CLG) caused by mycobacterial strains of the Mycobacterium simiae complex in Brazil. A 12-year-old non-neutered male Labrador Retriever dog was presented with a 2-week history of progressive painless cutaneous lesions. Ulcerated nodules with hematic crusts were observed on the dorsal surface of the right and left pinna and on the metacarpal, metatarsal, and digits.

Prevalence of sp. in Broiler Poultry Houses with Positive and Negative Pressure Ventilation Systems in Southern Brazil.

Coccidiosis is an enteric disease caused by protozoa of the genus and is of great economic relevance in industrial aviculture. The objective of this work was to determine the prevalence of sp. in broiler poultry houses with positive (System 1) and negative (System 2) pressure ventilation and assess the associated factors.

Plasma Cell Pododermatitis Associated With Feline Leukemia Virus (FeLV) and Concomitant Feline Immunodeficiency Virus (FIV) Infection in a Cat.

This report aims to describe one case of plasma cell pododermatitis associated with feline leukemia virus (FeLV) and concomitant feline immunodeficiency virus (FIV) infection in a cat. A 2-year-old, intact male, mixed-breed cat was presented with alopecia, skin peeling, and erythematous swelling in the left metacarpal paw pad. Swelling, softening, ulceration with secondary crusts, and erythematous to violaceous discoloration were observed in multiple metacarpal, metatarsal, and digital paw pads.

ASPIRATION BRONCHOPNEUMONIA BY IN A WILDLIFE EUROPEAN HARE () IN BRAZIL.

is a major cause of illness in hospitalized patients and the most important and common pathogen in nosocomial outbreaks worldwide. In animals, has been associated with respiratory infections in a group of minks, leading to pneumonia and acute mortality. This report documents a case of aspiration bronchopneumonia in a wild European hare caused by .

Prevalence of and factors associated with feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) in cats of the state of Santa Catarina, Brazil.

A cross-sectional study was conducted in 274 cats for determination of FeLV antigenemia and FIV seropositivity and factors associated with those infections in cats presented at the Veterinary Hospital of the Santa Catarina State University - UDESC (Brazil). Apparent prevalence for sick cats at the hospital population was 28.41% (95%CI 21.

Glycerol monolaurate in the diet of broiler chickens replacing conventional antimicrobials: Impact on health, performance and meat quality.

Glycerol monolaurate (GML), known as lauric acid, is a chemical compound formed from lauric acid and glycerol that presents strong antimicrobial activity. Therefore, our hypothesis is that MGL can replace conventional antimicrobials, being a new alternative to poultry farming. The aim of this study was to evaluate whether the addition of GML as a replacement for antibiotics could have positive effects on health and performance of broiler chickens.

Impairment of the phosphotransfer network and performance in broiler chickens experimentally infected by Eimeria spp.: The role of the oxidative stress.

The aim of this study was to evaluate whether infection Eimeria spp. in broiler chickens could negatively affect seric enzymes linked to adenosine triphosphate (ATP) metabolism and its relationship to oxidative stress. For this, 30 broiler chickens, 27 days-old, were divided into two groups (n = 15): the control group (C) and the group infected by Eimeria spp.

Fatal toxoplasmosis in an immunosuppressed domestic cat from Brazil caused by Toxoplasma gondii clonal type I.

The objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (FeLV). A domestic cat (Felis silvestris catus) presented intense dyspnea and died three days later. In the necropsy, the lungs were firm, without collapse and with many white areas; moderate lymphadenomegaly and splenomegaly were also observed.

Hepatic cholinesterase of laying hens naturally infected by Salmonella Gallinarum (fowl typhoid).

Salmonella is a facultative intracellular pathogen that may cause foodborne gastroenteritis in humans and animals consisting of over 2000 serovars. The serovar Salmonella Gallinarum is an important worldwide pathogen of poultry. However, little is known on the mechanisms of pathogenesis of Salmonella in chickens.

Ectonucleotidases and adenosine deaminase activity in laying hens naturally infected by Salmonella Gallinarum and their effects on the pathogenesis of the disease.

Salmonella Gallinarum is the etiologic agent of fowl typhoid that affects chickens and turkeys causing egg production drops, infertility, lower hatchability, high mortality, and as a consequence severe economic losses to the poultry industry. The alterations in NTPDase and adenosine deaminase (ADA) activities have been demonstrated in several inflammatory conditions; however, there are no data in the literature associated with this infection. Thus, the aim of this study was to evaluate the activities of NTPDase, 5'nucleotidase, and ADA in serum and hepatic tissue of laying hens naturally infected by Salmonella Gallinarum.

Fatal systemic toxoplasmosis in Valley quail (Callipepla californica).

An adult, captive raised male Valley quail (Callipepla californica) acquired by a southern Brazilian aviary suddenly showed severe apathy, dyspnea and diarrhea, and died 18 hours after the onset of illness. At necropsy, pale muscles and whitish areas in the heart, splenomegaly, hepatomegaly, and consolidated red lungs were observed. Histological findings were mainly mononuclear inflammation with necrosis of liver, heart, spleen, bone marrow and lung.