Publications by authors named "Renan Barcellos"
Article Synopsis
- Ecological conditions influence how organisms adapt at molecular, anatomical, and behavioral levels, essential for predicting competition outcomes and evolutionary paths.
- Recent technologies, such as RNAseq and computer vision, have advanced the study of molecular and behavioral responses, but anatomical studies have not kept pace.
- Using X-ray micro-computed tomography (micro-CT), we examined how Drosophila melanogaster male reproductive organs adapt to changes in larval density, revealing two main anatomical changes consistent with sexual selection theory.
Purpose: In the context of synchrotron microtomography using propagation-based phase-contrast imaging (XSPCT), we evaluated the performance of semiautomatic and automatic image segmentation of soft biological structures by means of Dice Similarity Coefficient (DSC) and volume quantification.
Methods: We took advantage of the phase-contrast effects of XSPCT to provide enhanced object boundaries and improved visualization of the lenses of the frog Thoropa miliaris. Then, we applied semiautomatic segmentation methods 1 and 2 (Interpolation and Watershed, respectively) and method 3, an automatic segmentation algorithm using the U-Net architecture, to the reconstructed images.