The Prognostic Value of Preoperative Plasma Fibrinogen and Neutrophil-to-Lymphocyte Ratio in Patients With Laryngeal Squamous Cell Carcinoma.

Objectives: Treatment effects in patients with laryngeal squamous cell carcinoma may vary significantly even among those with the same TNM stage. Routine preoperative blood and coagulation tests are economical and easily available hematological indicators. This study aimed to investigate the clinical predictive significance of pretreatment evaluation of plasma fibrinogen (FIB) level, neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) in patients with laryngeal carcinoma.

Identification of key candidate genes and pathways in anaplastic thyroid cancer by bioinformatics analysis.

Background: Anaplastic thyroid carcinoma (ATC) is a refractory and poor prognosis tumor Present study aimed to investigate the underlying biological functions and pathways involved in the development of ATC and to identify potential hub genes and candidate biomarkers of ATC.

Materials And Methods: Bioinformatics analyses were performed to identify the differentially expressed genes (DEGs) between ATC tissue samples and adjacent normal tissue samples. Protein-protein interaction (PPI) networks of the DEGs were constructed using Search Tool for the Retrieval of Interacting Genes online tool and Cytoscape software and divided into sub-networks using the Molecular Complex Detection (MCODE) plug-in.

[Longterm impact on swallowing quality-of-life after partial laryngectomy].

Objective: To discuss the longterm quality-of-life related to swallowing function after different partial laryngectomy.

Methods: The worldwide known swallow quality-of-life questionnaire (SWAL-QOL, Hongkong, Chinese edition); was used in this research to evaluate the swallowing QOL on 96 postoperative patients who underwent different kinds of partial laryngectomy more than one year before. The patients were divided into 4 groups: supracricoid partial laryngectomy-cricohyoidopexy (SCPL-CHP), supracricoid partial laryngectomy-cricohyoidoepiglottopexy (SCPL-CHEP), horizontal supraglottic partial laryngectomy (horizontal PL), and vertical partial laryngectomy (vertical PL).

[Comparison of postoperative deglutition disorder in patients with laryngeal carcinoma after different types of supracricoid partial laryngectomy].

Objective: To Evaluate the incidence rates and extents of deglutition disorder in patients with laryngeal carcinoma after different types of supracricoid partial laryngectomy.

Methods: Retrospective analysis of postoperative deglutition disorder in patients with laryngeal carcinoma after different types of supracricoid partial laryngectomy treated in our department from 2005 to 2009. The extents of postoperative deglutition disorder were evaluated using a homemade quantitative score table at 5-20 days postoperation.

[Comparing extended vertical partial laryngectomy and cricohyoidoepiglottopexy in the treatment of laryngeal carcinoma].

Objective: To compare the results of extended vertical partial laryngectomy (similar to modified supracricoid partial laryngectomy with cricohyoidoepiglottopexy) and cricohyoidoepiglottopexy in the treatment of laryngeal carcinoma.

Methods: Retrospectively analyzed on the results and prognosis in patients underwent extended vertical partial laryngectomy and cricohyoidoepiglottopexy between 1998 and 2005. The operation was similar to extended vertical partial laryngectomy.

[Establishment and preliminary application of polymerase chain reaction with confronting two-pair primers for the single nucleotide polymorphisms of metabolic enzymes].

Objective: To develop a simple, accurate, rapid, economic, large-scale detection method for the detection of single nucleotide polymorphisms (SNPs) metabolic enzymes, using polymerase chain reaction with confronting two-pair primers (PCR-CTPP).

Methods: The primers of CYP1A1 (A4889G), EPHX1 (A416G) and NQO1 (C609T) were designed for PCR-CTPP, and the PCR conditions were optimized. The results of genotyping were verified by DNA sequencing.

Orthogonal projection to latent structures combined with artificial neural networks in non-destructive analysis of Ampicillin powder.

A new method orthogonal projection to latent structures (O-PLS) combined with artificial neural networks is investigated for non-destructive determination of Ampicillin powder via near-infrared (NIR) spectroscopy. The modern NIR spectroscopy analysis technique is efficient, simple and non-destructive, which has been used in chemical analysis in diverse fields. Be a preprocessing method, O-PLS provides a way to remove systematic variation from an input data set X not correlated to the response set Y, and does not disturb the correlation between X and Y.

[Nondestructive quantitative analysis of Cofrel medicines by improved partial least squares-NIR spectroscopy].

In the present paper, improved partial least squares (PLS) method was used to parse near infrared (NIR) reflectance spectrum of Cofrel medicine. The contents of benproperine phosphate, a availability pharmaceutical in Cofrel medicine, were accurately nondestructively quantitatively predicted. The best number of principal components (PC) was designed by scores plot of samples in PC.

[Study on extraction of coumarins in Cnidium monieri by supercritical CO2 and separation of compositions analysis].

Objective: To analyze the chemical constituents of supercritical carbon dioxide extraction products from Cnidium monieri.

Method: Four-factor and three-level orthogonal experimental design was used to optimize the SFE conditions as guided by the content of total coumarins in the extract. The chemical constituents were separated and identified by recrystalization.

[Role of microvessel density on human middle ear cholesteatoma].

Objective: To explore the role of microvessel density in acquired middle ear cholesteatoma, to analyze it playing the possible role in proliferative ability of cholesteatoma epidermis.

Methods: The specimens from 15 cases acquired middle ear cholesteatoma patients having typical perforation in the posterior fold (the adjacent skin around perforation and the normal deep external ear skin) were examined by immunohistochemical ABC method. The microvessel density was analyzed by computer, and was compared with 20 cases non-cholesteatoma otitis media's the adjacent skin around perforation.

[Roles of epidermal growth factor and its receptor on the chronic tympanic membrane perforations].

Objective: To evaluate the possible roles of epidermal growth factor(EGF) and its receptor (EGFR) on the chronic tympanic membrane perforations.

Methods: A phosphate buffer saline of EGFR was administered to a Gelfoam pledget placed over chronic tympanic membrane perforations in guinea pigs. The EGFR of 10 specimens from the acquired middle ear cholesteatoma of the adjacent skin around perforation was examined by immunohistochemical SP method and computer image analysis.

[Studies on the chemistry of two new compounds of Hemistepta lyrata].

Aim: To study the chemical constituents of Hemistepta lyrata.

Methods: To separate compounds with various chromatography technology and to elucidate their structures by chemical and spectral analysis.

Results: Two compounds were isolated from Hemistepta lyrata and their structures were determined as hemiceramide (I), hemisterpene ether (II).