[Expression of human phosphodiesterase 3A gene using baculovirus expression system in insect cell].

Aim: To investigate the expression of recombinant human phosphodiesterase 3A (HPDE3A) using baculovirus expression system in Tn cell line.

Methods: The HPDE3A cDNA was recombined with baculovirus, and then the recombinant was transfected into Tn cell line. The expression of HPDE3A in Tn cell line was detected and identified by the RT-PCR, SDS-PAGE and Western blotting.

[Analysis of protamine content in patients with asthenozoospermia].

Objective: To study the significance of the change of protamine content in the management of male infertility.

Methods: Sperm nuclear proteins extracted from 199 infertile patients were analyzed by polyacrylamide gel-electrophoresis and scanning microdensitometry.

Results: Forty-two (21%) cases of the total number had normal sperm nuclear proteins and 157 (79%) had aberrant ones, which mainly presented the interruption of HPRR and abnormality of P2 protamine.

[Expression of rat protamine gene in MEL cells].

Objective: To investigate the expression of rat protamine (RP) gene in MEL cells and the effect on cell growth.

Methods: Eukaryotic expression plasmid pCR-3.1-RP was constructed and transfected into MEL cells.