Comparative genomics analysis of Acinetobacter baumannii multi-drug resistant and drug sensitive strains in China.

The incidence of multidrug-resistant Acinetobacter baumannii has posed a major challenge for clinical treatment. There is still a significant gap in understanding the mechanism causing multi-drug resistance (MDR). In this study, the genomes of 10 drug sensitive and 10 multi-drug resistant A.

Proteomic Analyses of Clinical Isolates to Identify Drug Resistant Mechanism.

is one of the main causes of nosocomial infections. Increasing numbers of multidrug-resistant cases have been reported in recent years, but its antibiotic resistance mechanism remains unclear. We studied 9 multidrug-resistant (MDR) and 10 drug-susceptible clinical isolates using Label free, TMT labeling approach and glycoproteomics analysis to identify proteins related to drug resistance.

Roles of the Hcp family proteins in the pathogenicity of 14028s.

The type VI secretion system (T6SS) is a new secretion system that is widely distributed among Gram-negative bacteria. The core component hemolysin-coregulated protein (Hcp) can be used as both its structural protein and secretory protein or chaperone protein. Studies on Hcp are important to elucidate the overall virulence mechanism of T6SS.

Genotyping of Salmonella with lineage-specific genes: correlation with serotyping.

Background: The bacterial genus Salmonella encompasses a large number of serotypes that are genetically very similar but biologically quite different, especially in pathogenic properties and host specificity. Serotyping has been used for the classification, identification, and epidemiological investigation due to its excellent discriminating power, but it cannot distinguish the different pathogenic lineages within a polyphyletic serotype. Additionally, very few institutions have the comprehensive set of antisera for typing.

Comparative genomic analysis between typhoidal and non-typhoidal Salmonella serovars reveals typhoid-specific protein families.

Background: The genus Salmonella contains more than 2600 serovars. While most cause a self-limiting gastroenteritis, four serovars, S. Typhi, S.

[Study on the Fast Testing Strategy for identifying the wild poliovirus I].

Objective: To explore the Fast Testing Sstrategy (FTS) for wild poliovirus I (WP1).

Methods: Epidemiological investigations were carried out on 671 students from WP1 epidemic areas in China. A set of real time RT-PCR assays, including panenterovirus testings (PE) assay, poliovirus serotypings (PS) assay and the assay distinguishing wild strain from vaccine strain of poliovirus I (DWV) were introduced into the screening program for WPV1 to replace the conventional RT-PCR, recommended by the China National Polio Laboratory (GNPL).

Helicobacter pylori in the oral cavity and gastric mucosa: a meta-analysis.

Background: Helicobacter pylori have been found in the oral cavity and stomach. This study is to establish whether there might be any associations between isolates of H. pylori in the oral cavity and those in the stomach by meta-analysis.

[Study on the etiology of hand-foot-mouth disease outbreaks in Beijing in 2007].

Objective: To identify the etiology of 8 human hand-foot-mouth disease (HFMD) outbreaks in Beijing, during May to July 2007.

Methods: Reverse transcription-polymerase chain reaction (RT-PCR) method was used to directly type the specimens including fluid from the herpes and throat swabs from the HFMD patients. Using RD cell lines, the collected stool specimens were cultured followed by typing.

[Human enterovirus 71 was isolated from specimens of hand foot mouth disease in Beijing in 2007].

Objective: Analyzing and identifying the type of enterovirus of human Hand Foot Mouth Disease (HFMD) outbreak in Daxing district in Beijing at the end of May in 2007.

Methods: The liquid of Herpes, throat swab and stool specimen were collected. Using reverse transcription-polymerase chain reaction (RT-PCR) method to amplify the enterovirus specific nucleotide acid fragment from specimens and virus positive cultures, the sensitivity of two methods was compared.

[Genetic characteristics of enterovirus 71 isolated in Beijing, 2006-2008].

Objective: To sequence and analyze the VP1 region of isolated enterovirus from different sources in Beijing, 2006-2008.

Methods: 9 EV71 were selected from the isolates identified through the specimen of human hand foot mouth disease (HFMD), acute flaccid paralysis (AFP) and healthy children in Beijing, 2006-2008. Reverse transcription-polymerase chain reaction (RT-PCR) method was used to amplify and sequence the whole VP1 gene of enterovirus.