Application of Half-Transected and Self-Pulling Esophagojejunostomy in Total Laparoscopic Gastrectomy for Gastric Cancer: A Safe and Feasible Technique.

Objective: This study introduces a technique for esophagojejunostomy with half transected and self-pulling (HTSP) and evaluates the safety, feasibility, and clinical results of this technique in totally laparoscopic total gastrectomy (TLTG).

Materials And Methods: From May 2019 to March 2021, 42 patients (HTSP group) who underwent HTSP-TLTG surgery in the Department of Abdominal Tumor Surgery of Jiangxi Cancer Hospital were included in this study. The control group consisted of 50 patients undergoing conventional TLTG surgery (conventional anastomosis group) performed by the same surgical team from March 2018 to March 2020.

Cerium-based nanoparticles triggered catalytic reaction for the colorimetric and ratiometric fluorimetric dual-signal sensing of vitamin C.

The construction of multi-modal detection methods has attracted widespread attention in the field of biosensing due to their high sensitivity and strong anti-interference ability. In this manuscript, we developed colorimetric and ratiometric fluorescence dual-signal optical methods based on cerium-based nanoparticles (Ce NPs) for the sensitive detection of vitamin C (VC). The catalysis of Ce NPs with excellent peroxidase-like activity upon the reaction of HO with OPD was occurred, promoting the oxidation of o-phenylenediamine (OPD) to generate 2,3-diaminophennazine (OPDox) with an obvious absorption peak at 420 nm and an emission peak at 565 nm.

MicroRNA-301b-3p facilitates cell proliferation and migration in colorectal cancer by targeting HOXB1.

Previous studies revealed that miR-301b-3p was essential to the onset and development of several cancers, but the implied functions of miR-301b-3p in colorectal cancer (CRC) remained largely unclear. The current study is aimed to exploring the potential roles and possible mechanism of miR-301b-3p in CRC. The abundance of miR-301b-3p and HOXB1 in CRC clinical specimens and cell lines was verified using RT-qPCR.

Silencing of the ARK5 gene reverses the drug resistance of multidrug-resistant SGC7901/DDP gastric cancer cells.

For several years, the multidrug resistance (MDR) of gastric cancer cells has been a thorny issue worldwide regarding the chemotherapy process and needs to be solved. Here, we report that the ARK5 gene could promote the multidrug resistance of gastric cancer cells in vitro and in vivo. In this study, LV-ARK5-RNAi lentivirus was used to transfect the parental cell line SGC7901 and MDR cell line SGC7901/DDP to construct a stable model of ARK5 interference.

Development of High-Performance Enamel Coating on Grey Iron by Low-Temperature Sintering.

In this study, we report on a low-temperature sintered enamel coating with a high-strength bonding and wear-resistance that protected a grey cast iron substrate. The SiO₂⁻Al₂O₃⁻B₂O₃ composited prescription for the enamel coating was modified by the partial substitutions of SiO₂ for B₂O₃ and alkali metals for Li₂O. The optimized enamel coating was prepared by sintering at a relatively low temperature (730 °C) for seven minutes.

Inhibition of iNOS alleviates cognitive deficits and depression in diabetic mice through downregulating the NO/sGC/cGMP/PKG signal pathway.

Diabetes mellitus often results in a number of complications involving impaired brain function, including cognitive deficits and depression. However, the potential mechanisms for diabetes-related cognitive deficits and depression are not fully understood. Neurons in the hippocampal, cortical and amygdala functional regions are more susceptible to damage during hyperglycemia.

Etiology of empyema thoracis and parapneumonic pleural effusion in Taiwanese children and adolescents younger than 18 years of age.

We analyzed blood and pleural fluid samples from 89 Taiwanese children with empyema thoracis and parapneumonic pleural effusion. Streptococcus pneumoniae was the major pathogen, identified in 12 children by bacterial culture and 53 children by molecular techniques, and serotype 19A was the dominant serotype. Also noteworthy was the detection of pneumococcal serotype 1, Haemophilus influenzae and Mycoplasma pneumoniae in these children.

Comparison of self-microemulsifying drug delivery system versus solid dispersion technology used in the improvement of dissolution rate and bioavailability of vinpocetine.

The objective of this study is to compare the differences between self-microemulsifying drug delivery system (SMEDDS) and solid dispersion (SD) technology used to improve the dissolution rate and bioavailability of vinpocetine (VIP). The formulation of VIP-SMEDDS was composed of Labrafac, oleic acid, Cremophor EL, Transcutol P, and gum acacia which was used as solid absorbent. VIP-SD was prepared using poloxamer F68 as the carrier.

Preparation of budesonide-poly (ethylene oxide) solid dispersions using supercritical fluid technology.

The purpose of this study was to investigate the possibility of preparing solid dispersions of the poorly soluble budesonide by supercritical fluid (SCF) technique, using poly (ethylene oxide) (PEO) as a hydrophilic carrier. The budesonide-PEO solid dispersions were prepared, using supercritical carbon dioxide (SC CO(2)) as the processing medium, and characterized by scanning electron microscopy (SEM), powder X-ray diffraction (PXRD), differential scanning calorimetry (DSC), solubility test and dissolution test in order to understand the influence of the SCF process on the physical status of the drug. The endothermic peak of budesonide in the SCF-treated mixtures was significantly reduced, indicating that budesonide was in amorphous form inside the carrier system.

Characteristics of Chlamydia trachomatis infection in hospitalized infants with lower respiratory tract infection.

Background And Purpose: To study the epidemiology, presentation and laboratory findings of Chlamydia trachomatis pneumonia in hospitalized infants younger than 6 months.

Methods: Between January 2001 and December 2005, infants younger than 6 months admitted to the children's medical center of Taipei Veterans General Hospital with the diagnosis of acute bronchiolitis, bronchopneumonia or pneumonia were prospectively studied. Chest radiograph findings were reviewed in all patients.

Chitosan-based controlled porosity osmotic pump for colon-specific delivery system: screening of formulation variables and in vitro investigation.

A microbially triggered colon-targeted osmotic pump (MTCT-OP) has been studied. The gelable property at acid condition and colon-specific biodegradation of chitosan were used to: (1) produce the osmotic pressure, (2) form the drug suspension and (3) form the in situ delivery pores for colon-specific drug release, respectively. The scanning electron microscopy (SEM) study and the calculation of membrane permeability were applied to elucidate the mechanism of MTCT-OP.

Effects of Erigeron breviscapus ethanol extract on neuronal oxidative injury induced by superoxide radical.

Antioxidant activity of the ethanol extract of Erigeron breviscapus (EEEB) were studied by using neuron oxidative injury model induced by superoxide radical. EEEB at the dose of 10-140 mug/ml reduced significantly the lipid peroxidation levels and lactic dehydrogenase (LDH) release from neuron exposed to superoxide radical (generated by xanthine and xanthine oxidase). These results suggest that neuroprotective actions of EEEB may be due to its antioxidant or radical scavenging activity.

Preparation and in vivo evaluation of huperzine A-loaded PLGA microspheres.

Huperzine A-loaded microspheres composed of poly(D,L-lactide-co-glycolide) were prepared by an ONV emulsion solvent evaporation method. The characterization of the microspheres such as drug loading, size, shape and release profile was described. The in vitro release in the initial 7 days was nearly linear with 10% released per day.

Effect of scutellarin on nitric oxide production in early stages of neuron damage induced by hydrogen peroxide.

The aims of the present study were to investigate the regulatory function of scutellarin on production of nitric oxide (NO) as well as activities of constitutive NO synthase (cNOS) and inducible NO synthase (iNOS) in early stages of neuron damage induced by hydrogen peroxide. Direct detection of NO production was performed on primary cultures of living rat neuronal cells with an electrochemical sensor. Hydrogen peroxide significantly increased culture supernatant levels of NO, the total integral value of the defined areas (500-6500 sxpA) reached 3.

[Effects of protease inhibitors on intestinal absorption of insulin].

Aim: To study the effects of protease inhibitors on the large and small intestinal absorption of insulin in rats and to explore the mechanism of various protease inhibitors in different intestinal regions.

Methods: The intestinal absorption of insulin was evaluated by its hypoglycemic effect and serum insulin level using an in situ loop method with the washing treatment.

Results: Administration of insulin alone did not decrease the glucose level at either intestinal region with or without the washing treatment.

Topical delivery of different acyclovir palmitate liposome formulations through rat skin in vitro.

The objective of this investigation was to examine the permeation of acyclovir palmitate from various liposome formulations through hairless rat skin in vitro. The penetrated amount, permeability and intradermal retention of ACV-C16 were compared among various lipid compositions and different vesicle charges. We found that all of the liposome formulations resulted in higher flux and permeability of ACV-C16 than a common ointment form.

Potential utility of various protease inhibitors for improving the intestinal absorption of insulin in rats.

The aim of the investigation was to study the effects of protease inhibitors on the absorption of insulin in-situ from closed small and large intestinal loops in rats and to investigate the mechanism of various protease inhibitors in different intestinal loops. The intestinal absorption of insulin was evaluated by its hypoglycaemic effect and serum insulin level in the presence or absence of luminal contents. No marked hypoglycaemic effect was observed after administration of insulin alone in either region in the presence or absence of luminal contents.

[Effects of liposomes formulation and preparation method on the stability of acyclovir palmitate liposomes].

Aim: To study the effects of various liposomes formulations and preparation methods on the stability of acyclovir palmitate (ACV-C16) liposomes on storage at 4 degrees C and 25 degrees C over a 6 months period.

Methods: The mean particle size, Zeta potential, pH and leaking ratio of ACV-C16 liposomes were the parameters chosen to indicate the stability of liposomes. All of the parameters were compared among various lipid compositions [egg lecithin/cholesterol/hosphatidylserine (PC/CH/PS), egg lecithin/cholesterol/stearylamine (PC/CH/SA), egg lecithin/cholesterol/cholesteryl sulphate (PC/CH/CS), bovine brain ceramides/cholesterol/palmitic acid/cholesteryl sulphate (CM/CH/PA/CS)], different preparation methods (film dispersing, reverse phase evaporation, dehydration/rehydration), charges (positive, negative), as well as among multilamellar vesicles liposomes (MLV), large unilamellar vesicles liposomes (LUV) and dehydration/rehydration vesicles liposomes (DRV).