[Development and effectiveness evaluation of time-resolved fluoroimmunoassay kit for detection of ].

Objective: To develop a kit of time-resolved fluoroimmunoassay (TRFIA) for detection of protein SjP38, and evaluate its effectiveness.

Methods: The anti 9G7 SjP38 monoclonal antibody was used as the capture anti-body coated with 96-hole plate, and the Eu3 labeled 1A6 monoclonal antibody was used as the detection antibody to establish the TRFIA SjP38 kit. In addition, the accuracy, sensitivity, precision, stability and coincidence rate to pathogenic diagnosis of the kit were evaluated.