Effects of oxidative stress on the changes of viability of Paeonia lactiflora seeds with different water content before and after cryopreservation.

Cryopreservation in liquid nitrogen (LN) is an efficient long - term seed preservation strategy and water content is one of the main factors affecting seed viability after cryopreservation. In this study, Paeonia lactiflora seeds with varying water content were used as materials to determine changes in the antioxidant system before and after cryopreservation. When the water content of P.

Exploring the relationship between pollen viability and inclusion in Paeonia lactiflora after cryopreservation.

Pollen, as the male gametophyte, carries half of plant genetic information and is an important source of germplasm. The cryopreservation of pollen can not only preserve germplasm, but also solve the problem of time and space barrier in crossbreeding. So it is of great significance to explore the mechanism of pollen viability maintenance after cryopreservation.

Cryopreservation of herbaceous Asteraceae seeds: Effects of seed reserves on seed germination and seedling regrowth.

Carbohydrate, lipid and protein, the three main storage reserves of seeds, are the main sources of raw materials and energy for seed germination and early growth stage of seedlings. In this study, the seed of 16 Asteraceae herbaceous ornamentals were used to compare the changes in seed germination, seedling growth indexes and four major storage reserves of seeds before and after cryopreservation. After cryopreservation, seed germination and seedling growth were preserved in most species (11/16), while one species showed significantly improved post-thaw germination and four had decreased germination.

Ca participates in programmed cell death by modulating ROS during pollen cryopreservation.

After cryopreservation, the Ca content increased, which affected the intracellular ROS content, then participated in the occurrence of programmed cell death in pollen. Programmed cell death (PCD) is one of the reasons for the decline in pollen viability after cryopreservation. However, the role of calcium ions (Ca) in PCD during pollen cryopreservation has not been revealed in the existing studies.

Changes of pollen viability after preservation of Paeonia lactiflora in different provenances.

Pollen contains all the haploid genetic information of species and is of great significance to preserve germplasm resources safely and effectively. The acquisition of high quality materials is a very important step in germplasm preservation. This study compared the viability and physiological condition of Paeonia lactiflora pollen from several provenances after preservation, to explore the effect of provenance difference on pollen viability and physiological responses after preservation.

The ROS-associated programmed cell death causes the decline of pollen viability recovered from cryopreservation in Paeonia lactiflora.

After cryopreservation, the occurrence of apoptosis-like programmed cell death events induced by the accumulation of ROS reduces pollen viability. Cryopreservation, as a biotechnological means for long-term preservation of pollen, has been applied to many species. However, after cryopreservation, the viability of pollen significantly decreases via a mechanism that is not completely clear.

Changes of pollen viability of ornamental plants after long-term preservation in a cryopreservation pollen bank.

This study determined the changes in pollen viability of 102 species/cultivars of ornamental plants (affiliated to 32 genera of 14 families) following long-term liquid nitrogen storage in a cryopreservation pollen bank. The goal was to provide information on the safety and stability of pollen cryopreservation technology. Fresh pollen at the time of storage was used as the control, and the study examined the pollen viability of ornamental plants cryopreserved for 8, 9, or 10 years.