Craig-Hückel Hybrid Aromatic Metalla-dehydro[11]annulenes Constructed by a Formal [10+1] Cycloaddition Reaction.

Aromatic metalla-annulenes are important aromatic compounds, research into which has been mainly concentrated on metal-benzenes and their lower homologues. Reports on their superior homologs are rare, and this has greatly limited the systematic study of their properties. In this work, a series of osma-dehydro[11]annulenes with good air and thermal stability were prepared in high yields through a simple [10+1] strategy, by incorporating a metal fragment into conjugated ten-carbon chains in a one-pot reaction.

Mining the genome sequence for novel enzyme activity: characterisation of an unusual member of the hormone-sensitive lipase family of esterases from the genome of Streptomyces coelicolor A3 (2).

The sequence of the estA gene (locus SCO 7131) of S. coelicolor A3 (2) suggested that it might differ in substrate specificity from other characterised members of the hormone-sensitive lipase (HSL) family of lipases and esterases. This difference may be attributed to the unique substitutions within the conserved motifs of the family.

A cold-active esterase of Streptomyces coelicolor A3(2): from genome sequence to enzyme activity.

The genome sequence of Streptomyces coelicolor A3(2) contains 51 putative lipase and esterase genes mostly of unknown function. The gene estB (locus SCO 6966) was expressed as a His-tagged protein in E. coli.