Protein glycosylation is one of the most important post-translational modifications, implicated in the development of various diseases, including neurodegenerative diseases, diabetes, and cancers. However, the low content of glycoproteins in biological samples, the diversity and heterogeneity of glycan structures, and insensitive detection methods make glycosylation analysis challenging. As a result, efficient enrichment of glycopeptides from complex samples is a critical step.
View Article and Find Full Text PDFEthnopharmacological Relevance: Lichong decoction (LD) is extensively employed in the treatment of uterine leiomyoma (ULM), demonstrating remarkable clinical effectiveness with an absence of notable adverse reactions. Its composition aligns with the traditional Chinese medicine (TCM) etiology of ULM, making it a highly suitable therapy. Nonetheless, the precise mechanisms underlying its therapeutic actions remain to be fully elucidated.
View Article and Find Full Text PDFBackground: Health education and promotion are recognized as effective strategies for fostering healthy ageing, reducing the disease burden, and addressing health inequalities, particularly when delivered through digital media. Primary care workers are often regarded as the key providers of these interventions. Despite the strong practical significance and substantial individual demand, the use of digital media for delivering health promotion practices was not widespread in China.
View Article and Find Full Text PDFThe flowers of distylous plants exhibit two distinct morphologies that facilitate precise pollen transfer. Averrhoa carambola, a woody plant characterized by distyly, has an unclear molecular regulatory mechanism underlying this trait. Its prolonged flowering period and substantial flower production render it an excellent model for investigating the distylous syndrome.
View Article and Find Full Text PDFComp Immunol Microbiol Infect Dis
December 2024
Reticuloendotheliosis virus (REV) is a retrovirus in poultry that can atrophy immune organs and cause immunosuppression and tumor diseases. Our previous results revealed that, in the spleen of SPF chickens infected with REV-SNV, gga-miR-222b-5p expression was upregulated at 7, 14 and 21 dpi, and MAPK10 expression was downregulated, both of which were negatively correlated, with a targeted relationship between the two at 28, 35 and 42 dpi. To understand and analyze the expression patterns of MAPK10 and gga-miR-222b-5p in infected chickens at different times and in different immune organs, qRT-PCR was used to analyze the spleen, bursa of Fabricius and thymus samples of SPF chickens at 7, 14, 21, 28, 35 and 42 dpi.
View Article and Find Full Text PDFNano-formulated pesticides are increasingly desired to control the insect pest and plant disease with superior efficacy for guaranteeing the high yield and quality in crop production. However, the impact of nanocarrier morphology on pesticide resistance against rainwash, photolysis, and overall pesticide bioactivity remains unknown. In this work, a series of well-defined and morphology-controllable polymer nanocarriers for pesticide are fabricated through polymerization-induced self-assembly.
View Article and Find Full Text PDFHot-carrier relaxation above the bandgap results in significant energy losses, making the extraction of hot carriers a critical challenge for efficient hot-carrier photocurrent generation in devices. In this study, we observe long-lived hot carriers in the metal-halide perovskite multiple quantum wells, (BA)(MA)PbI (n = 3), and demonstrate effective hot-hole photocurrent generation using 2D MoS₂ as an extraction layer. A high external quantum efficiency of short-circuit hot-carrier photocurrent of up to 35.
View Article and Find Full Text PDFBackground: Muscle atrophy is a condition of the skeletal muscular system closely related to inflammation and significantly affects a person's quality of life and physical activity. It is characterized primarily by the progressive loss of muscle mass, strength, and function. Plumbagin (PB), the main bioactive component of the traditional Chinese medicine Plumbago zeylanica L.
View Article and Find Full Text PDFInduction of parthenogenesis (embryo formation from unfertilized egg cells) by embryogenic transcription factors is associated with twin formation at high frequencies, and involves two distinct mechanisms. Synthetic apomixis has been achieved through the induction of parthenogenesis by ectopic expression of the Baby Boom family of transcription factors. An associated phenomenon from this process is the formation of polyembryony including twin progeny at high frequencies, but the underlying mechanisms have not been explored.
View Article and Find Full Text PDFAdv Healthc Mater
November 2024
Adhesive hydrogels characterized by structural properties similar to the extracellular matrix, excellent biocompatibility, controlled degradation, and tunable mechanical properties have demonstrated significant potential in biomedical applications, including tissue engineering, biosensors, and drug delivery systems. These hydrogels exhibit remarkable adhesion to target substrates and can be rationally engineered to meet specific requirements. In recent decades, adhesive hydrogels have experienced significant advancements driven by the introduction of numerous multifunctional design strategies.
View Article and Find Full Text PDFFront Cell Dev Biol
October 2024
CHAC1, an essential regulator of oxidative stress and ferroptosis, is increasingly recognized for its significant roles in these cellular processes and its impact on various human diseases and cancers. This review aims to provide a comprehensive overview of CHAC1's molecular functions, regulatory mechanisms, and effects in different pathological contexts. Specifically, the study objectives are to elucidate the biochemical pathways involving CHAC1, explore its regulatory network, and discuss its implications in disease progression and potential therapeutic strategies.
View Article and Find Full Text PDFThis study aimed to identify potential markers that can predict Parkinson's disease with mild cognitive impairment (PDMCI). We retrospectively collected general demographic data, clinically relevant scales, plasma samples, and neuroimaging data (T1-weighted magnetic resonance imaging (MRI) data as well as resting-state functional MRI [Rs-fMRI] data) from 173 individuals. Subsequently, based on the aforementioned multimodal indices, a support vector machine was employed to investigate the machine learning (ML) classification of PD patients with normal cognition (PDNC) and PDMCI.
View Article and Find Full Text PDFBackground: Iron is an essential element for thyroid function. However, no study focuses on the association between iron and thyroid in individuals with obesity. Our research aimed to investigate the iron status in relation to baseline thyroid hormone levels and after laparoscopic sleeve gastrectomy (LSG).
View Article and Find Full Text PDFBackground: Nursing shortage is a global issue. Turnover intention and life satisfaction are significant predictors of turnover. The specific nature of nursing and stressful work schedules lead to impaired professional quality of life (ProQOL), and existing studies have confirmed the effect of a dimension of ProQOL (such as secondary trauma stress, burnout and compassion satisfaction) on turnover intention and life satisfaction.
View Article and Find Full Text PDFBackground: Neuroblastoma (NB), a prevalent extracranial solid tumor in children, stems from the neural crest. Omics technologies are extensively employed in NB, and We analyzed published articles on NB omics to understand the research trends and hot topics in NB omics.
Method: We collected all articles related to NB omics published from 2005 to 2023 from the Web of Science Core Collection database.
Astrocytic processes minutely regulate neuronal activity via tripartite synaptic structures. The precision-tuning of the function of astrocytic processes is garnering increasing attention because of its significance in promoting brain repair following ischemic stroke. Microdomain calcium (Ca) transients in astrocytic processes are pivotal for the functional regulation of these processes.
View Article and Find Full Text PDFXi Bao Yu Fen Zi Mian Yi Xue Za Zhi
October 2024
Objective To prepare a Fc-silent chimeric antibody against human CD36 and analyse its bioactivity and phagocytic effect on CD36(+) platelets. Methods The genes encoding V and V fragments of the monoclonal antibody (mAb) 32-106 were obtained through RNA extraction from hybridoma cell lines, PCR amplification and sequence analysis. By using gene synthesis and recombination techniques, the vectors expressing light and heavy chains of the chimeric antibody against human CD36 were constructed.
View Article and Find Full Text PDFCD36, also known as glycoprotein IV, is classified into two distinct subgroups based on the presence or absence of its expression on monocytes. The CD36 gene spans approximately 50,000 base pairs. Historically, research has focused on identifying CD36 mutations through Sanger sequencing and next-generation sequencing (NGS), with limited exploration of haplotypes.
View Article and Find Full Text PDFBackground And Aims: Total bile acid (TBA) is associated with portal hypertension, a risk factor for post-hepatectomy liver failure (PHLF). We conducted this study to clarify whether TBA is also associated with PHLF in patients with hepatocellular carcinoma (HCC).
Methods: We recruited patients with HCC and Child-Pugh class A, who underwent liver resection, and applied multivariate analyses to identify risk factors for PHLF.
The sugar and organic acid content significantly impacts the flavor quality of star fruit, and it undergoes dynamic changes during development. However, the metabolic network and molecular mechanisms governing the formation of sugar and organic acid in star fruit remain unclear. In this study, 23 of 743 components were detected by metabonomic analysis.
View Article and Find Full Text PDFIndian jujube (Ziziphus mauritiana) holds a prominent position in the global fruit and pharmaceutical markets. Here, we report the assemblies of haplotype-resolved, telomere-to-telomere genomes of autotetraploid wild and cultivated Indian jujube plants using a 2-stage assembly strategy. The generation of these genomes permitted in-depth investigations into the divergence and evolutionary history of this important fruit crop.
View Article and Find Full Text PDFAdv Sci (Weinh)
November 2024
Functional coatings, including organic and inorganic coatings, play a vital role in various industries by providing a protective layer and introducing unique functionalities. However, its design often involves time-consuming experimentation with multiple materials and processing parameters. To overcome these limitations, data-driven approaches are gaining traction in materials science.
View Article and Find Full Text PDFAluminum nanoparticles (nAl) have received sustained interest due to their higher reactivity than micron aluminum particles (mAl). However, in practice, the densities of explosive formulations with nAl are far smaller than those with mAl, which greatly undercuts the energy release performance. To take advantages of both kinds of Al particles, in situ integration of mAl@nAl composites was proposed and evaluated.
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