Single-cell profiling of the microenvironment in decidual tissue from women with missed abortions.

Objective: To define the decidual microenvironment in euploid and aneuploid missed abortions and elective termination of pregnancies.

Design: Prospective, multicenter, observational study.

Setting: Tertiary hospital and descriptive analysis of transcriptomic data.

Preparation of monoclonal antibody and development of an enzyme-linked immunosorbent assay for the detection of ceftiofur in animal-derived foods.

Ceftiofur (CEF) residues in animal-derived foods are of great concern to farmers, regulatory agencies and consumers. In this study, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was established to quickly monitor CEF residues in edible animal tissues using an easy sample preparation procedure. A monoclonal antibody, 4D5, against CEF has been produced at first, which had IC values for CEF, ceftriaxone, cefquinome, cefotaxime and desfuroylceftiofur of 0.

Development of a monoclonal-based ic-ELISA for the determination of kitasamycin in animal tissues and simulation studying its molecular recognition mechanism.

To monitor the residue of kitasamycin (KIT), a monoclonal antibody against KIT was prepared, and a 50% inhibition concentration (IC) of 5.7 ± 1.4 μg/L was achieved with the most sensitive antibody, KA/2A9, by optimizing ELISA conditions.

DNA methylation and RASSF4 expression are involved in T-2 toxin-induced hepatotoxicity.

T-2 toxin is a secondary metabolite produced by Fusarium species and commonly contaminates food and animal feed. T-2 toxin can induce hepatotoxicity through apoptosis and oxidative stress; however, the underlying mechanism is not clear. Recent studies indicated that RASSF4, a member of the RASSF family, participates in cell apoptosis and some cancers due to its inactivation via DNA hypermethylation.

MicroRNA-10b inhibits proliferation, migration and invasion in cervical cancer cells via direct targeting of insulin-like growth factor-1 receptor.

MicroRNAs are deregulated in numerous types of human cancers and have crucial roles in the carcinogenesis and progression of human cancers. MicroRNA-10b (miR-10b) has been studied in several types of human cancer. However, the expression and roles of miR-10b in cervical cancer remain unknown.

Development and characterization of drug-loaded biodegradable PLA microcarriers prepared by the electrospraying technique.

Biodegradable particles are extremely useful in the development of novel drug delivery systems. Recent studies have suggested that morphology can influence the mechanisms of drug delivery in many ways. In the present study, biodegradable microparticles with different morphologies were prepared from poly(L‑lactide) (PLA) using the electrospraying technique.

Distribution of human papillomavirus genotype and cervical neoplasia among women with abnormal cytology in Beijing, China.

Objective: To understand the relationship between human papillomavirus (HPV) genotype distribution and cervical neoplasia among women with abnormal cytology in Beijing.

Methods: In a study in Beijing, China, participants underwent a gynecologic examination between September 2006 and December 2009. Those with abnormal cytology underwent colposcopy, biopsy, and HPV testing.

[Expression of orphanin in adenomyosis and its relationship with dysmenorrhea].

Objective: To study the expression of nociceptin in adenomyosis tissue and understand its relationship with the severity of dysmenorrhea.

Methods: The myometrial specimens were collected from 55 patients undergoing hysterectomy at our hospital during December 2007 and June 2008. Their mean age was 41 - 52 years old.