Objective: This study aimed to investigate the regulation of histone-like nucleoid structuring protein (H-NS) on biofilm formation and cyclic diguanylate (c-di-GMP) synthesis in RIMD2210633.
Methods: Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining, quantification of c-di-GMP, quantitative real-time polymerase chain reaction, LacZ fusion, and electrophoretic-mobility shift assay.
Results: The deletion of enhanced the biofilm formation and intracellular c-di-GMP levels in RIMD2210633.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
October 2011
Aim: Prepare the rabbit antiserum against gecko japonicus Hoxc10 and to identify its properties.
Methods: Prokaryotic expression vector of g-Hoxc10 were constructed and then transform into E.coli (BL21).