Adaptive optics light-sheet microscopy based on direct wavefront sensing without any guide star.

We propose an adaptive optics light-sheet fluorescence microscope (AO-LSFM) for closed-loop aberrations' correction at the emission path, providing intrinsic instrumental simplicity and high accuracy when compared to previously reported schemes. The approach is based on direct wavefront sensing, i.e.

Linear controller error budget assessment for classical adaptive optics systems.

Understanding limitations of adaptive optics (AO) systems is crucial when designing new systems. In particular, analyzing the potential of different controllers is of great interest for the upcoming AO systems of the very large telescopes (VLTs) and extremely large telescopes (ELTs). This paper thus details a complete error budget assessment formalism, based on analytic formulas involving the disturbance temporal power spectral density (PSD) and the controller transfer function, and is applicable to any linear controller.