A multiple shoot induction system for peptide-mediated gene delivery into plastids in , , and ×.

The plastid is a promising target for the production of valuable biomolecules via genetic engineering. We recently developed a plastid-specific gene delivery system for leaves or seedlings using KH-AtOEP34, a functional peptide composed of the polycationic DNA-binding peptide KH and the plastid-targeting peptide OEP34. Here, we established a liquid culture system for inducing multiple shoots in the model plants and and the crop plant strawberry (×) and tested the use of these plant materials for peptide-mediated gene delivery to plastids.