Interactions between lncRNAs and cyclins/CDKs complexes; key players in determining cancer cell response to CDKs inhibitors.

Transcription takes place over a significant portion of the human genome. However, only a small portion of the transcriptome, roughly 1.2%, consists of RNAs translated into proteins; the majority of transcripts, on the other hand, comprise a variety of RNA families with varying sizes and functions.

Exosome-mediated delivery of CRISPR-Cas9: A revolutionary approach to cancer gene editing.

Several molecular strategies based on targeted gene delivery systems have been developed in recent years; however, the CRISPR-Cas9 technology introduced a new era of targeted gene editing, precisely modifying oncogenes, tumor suppressor genes, and other regulatory genes involved in carcinogenesis. However, efficiently and safely delivering CRISPR-Cas9 to cancer cells across the cell membrane and the nucleus is still challenging. Using viral vectors and nanoparticles presents issues of immunogenicity, off-target effects, and low targeting affinity.

Targeting breast tumor extracellular matrix and stroma utilizing nanoparticles.

Breast cancer is a complicated malignancy and is known as the most common cancer in women. Considerable experiments have been devoted to explore the basic impacts of the tumor stroma, particularly the extracellular matrix (ECM) and stromal components, on tumor growth and resistance to treatment. ECM is made up of an intricate system of proteins, glycosaminoglycans, and proteoglycans, and maintains structural support and controls key signaling pathways involved in breast tumors.

Broad Autism Phenotype Questionnaire - Translation and Validation in a South Indian Language.

Background And Objectives: Broad Autism Phenotype (BAP) refers to specific behaviors or minor deficits in social/communication skills not meeting the diagnostic criteria for autism spectrum disorders (ASDs). The BAP Questionnaire (BAPQ) measures aloof personality (AP), rigid personality (RP), and pragmatic language (PL) deficits of BAP. The study objective is to translate and validate BAPQ into Malayalam, a South Indian language, and to establish new cut-off scores for detecting BAP in the Indian population.

Deciphering Annona's Anticancer Potential: Comparative Analysis of Crude Extract Versus Nanoformulation on SCC-25 Oral Cancer Cells Through cell Viability, Apoptosis, Cell Cycle, ROS, and MMP Analysis.

Introduction: Annona muricata contains acetogenins, which have shown promising anticancer activity against various cell lines. This study aims to evaluate and compare the anticancer activity of the crude extract of Annona muricata and its nano formulation on Squamous Cell Carcinoma-25 (SCC-25) oral cancer cell lines.

Methods: The crude extract of Annona muricata was prepared using standard extraction techniques, while its nano formulation was synthesized through nanoparticle fabrication methods.

Role of inorganic ions in wound healing: an insight into the various approaches for localized delivery.

Recently, the role of inorganic ions has been explored for its wound-healing applications. Ions do play key role in the normal functioning of the skin, including the epidermal barrier property, maintaining redox balance, enzymatic activities, tissue remodeling, etc. The care of chronic wounds is a concern and new cost-effective therapeutic strategies that modulate the wound microenvironment and cell behaviour are needed.

ADVANCEMENTS IN MINIMALLY INVASIVE SURGERY: A COMPREHENSIVE ANALYSIS OF ROBOTIC SURGERY, ENDOSCOPIC TECHNIQUES, AND NATURAL ORIFICE TRANSLUMENAL ENDOSCOPIC SURGERY.

The term "Natural Orifice Transluminal Endoscopic Surgery" (NOTES) defines a surgical approach that leverages the body's natural orifices to access the abdominal cavity, presenting a patient-centric perspective by highlighting its potential to eliminate abdominal wall aggression, mitigate postoperative discomfort, and offer benefits comparable to laparoscopic surgery. This comprehensive paper aims to not only review the existing landscape of NOTES techniques but also to propose advancements in flexible tools augmenting established endoscopic platforms, while also exploring the revolutionary concept of robotic structures grounded in micromechatronics and communication technologies. The thorough analysis encompasses the assessment of advantages and limitations associated with flexible devices and robotic platforms, coupled with an in-depth evaluation of the current array of devices used in NOTES, informed by pertinent literature.

Ascorbic acid-loaded gellan-g-poly(ethylene glycol) methacrylate matrix as a wound-healing material.

Ascorbic acid (AA) is one of the important biomolecules involved in all phases of wound healing. The aim of this study was to develop a new hydrogel system that offers topical delivery of ascorbic acid to wounds during wound care management. In this work, we grafted poly (ethylene glycol) methacrylate onto a renewable biopolymer gellan, and the graft copolymer (GPMA) formed was crosslinked covalently and ionically, and used as a matrix for delivering AA to the wounds.

Proline conjugated chitosan as wound healing material: In vitro studies on the influence of the scaffold on collagen production and wound healing.

The present study reports the development of L-proline conjugated chitosan scaffold for wound healing application. Proline plays a beneficial role in collagen synthesis, and as a biochemical, it has the potential to modulate wound healing. In this regard, amino acid L-proline was conjugated onto chitosan, and the scaffolds were synthesised.

Characterization and Assessment of 2, 4-Diacetylphloroglucinol (DAPG)-Producing VSMKU3054 for the Management of Tomato Bacterial Wilt Caused by .

Microbial bio-products are becoming an appealing and viable alternative to chemical pesticides for effective management of crop diseases. These bio-products are known to have potential to minimize agrochemical applications without losing crop yield and also restore soil fertility and productivity. In this study, the inhibitory efficacy of 2,4-diacetylphloroglucinol (DAPG) produced by VSMKU3054 against was assessed.

Assessment of knowledge and awareness of forensic odontology among dentists in Tamil Nadu - A systematic review.

Aim: The aim of the study is to analyze the knowledge, attitude and practice of forensic odontology among dental practitioners in Tamil Nadu.

Materials And Methods: Forensic odontology has immense importance in examining dental evidence and in the identification of victims of mass disaster, abuse or organized crimes. Hence, a cross-sectional survey study was conducted in a sample of 252 dental practitioners practicing in various cities in Tamil Nadu.

Activation Energy Impact on Flow of AA7072-AA7075/Water-Based Hybrid Nanofluid through a Cone, Wedge and Plate.

The present research investigates the effect of a heat source/sink on nanofluid flow through a cone, wedge, and plate when using a suspension of aluminium alloys (AA7072 and AA7075) as nanoparticles in base fluid water. The activation energy and porous material are also considered in the modelling. Using similarity transformations, the modelling equations were converted into an ordinary differential equation (ODEs) system.

The effect of acclimation temperature and optimal temperature gradient for egg and larvae of silver moony (Monodactylus argenteus) during the early ontogenesis.

The early life history of a fish species is regulated by temperature, the most critical environmental cue. Thus, identifying the gradient of temperature that optimises the early development of a species is a prerequisite for standardising hatchery technology. Silver moony, Monodactylus argenteus is a tropical brackishwater ornamental fish that holds scope for the Indian and Global ornamental fish industry.

Synthesis and evaluation of an alginate-methacrylate xerogel for insulin delivery towards wound healing applications.

Alginate is one of the most widely used biopolymer for wound healing. But poor mechanical strength and degradability limits its application especially as a drug-delivery matrix. The aim of this study was to develop stable alginate based scaffold for insulin delivery toward wound care.

High-entropy alloys for water oxidation: a new class of electrocatalysts to look out for.

High-entropy alloys (HEAs) with five or more elements can provide near-continuous adsorption energies and can be optimized for superior persistent catalytic activity. This report presents electrochemical water oxidation facilitated by employing graphene and FeCoNiCuCr HEA nanoparticle based composites prepared via the mechanical milling of graphite-metal powders. The composite efficiently facilitates water oxidation with a low overpotential of 330 mV at 10 mA cm-2, and high specific and mass activities (∼143 mA cm-2 and 380 mA mg-1, respectively, at 1.

Synthesis, characterization and anti-inflammatory properties of karanjin (Pongamia pinnata seed) and its derivatives.

Karanja (Pongamia pinnata) is a medicinal tree used in the Indian traditional ayurvedic system for treating several ailments. The seeds contain a unique furano-flavonoid karanjin, which has shown to possess many medicinal properties. Its usage at the clinical level is affected due to poor solubility and absorption.

Assessment of Quality of Platelet-Rich Plasma Produced With Desktop Centrifuge and Comparison With Standardized Commercially Available Platelet-rich plasma.

Aims: The aim of this study was to assess the quality of platelet-rich plasma (PRP) produced by in-house desktop centrifuge method and compare it with that of standardized commercial PRP.

Materials And Methods: REMI desktop centrifuge was used to prepare PRP and to compare with standardized commercial PRP by calculating the quantity of platelets using Beckman Coulter cell counter in 10 PRP samples and assessing the morphological quality of platelets using JEOL JEM transmission electron microscope (TEM).

Statistical Analysis Used: The test for platelet count in desktop PRP with the test value of therapeutic PRP was 12.

M-Mode Echomyography of Facial Muscle Function Following Facial Reanimation with Temporalis Muscle Galea Pedicled Flap: Analysis of Ten Cases with Review of Literature.

Introduction: Facial palsy is a condition where the patients lack voluntary movement on the affected side of the face and are not able to convey their emotions. Besides that, they also succumb themselves to social isolation. Various techniques have been devised to overcome this devastating problem.

Histidine and arginine conjugated starch-PEI and its corresponding gold nanoparticles for gene delivery.

The high surface energy of gold nanoparticles (GNPs) along with its unique physical and chemical properties delivers it as an effective nonviral gene delivery platform for anticancer treatments. GNPs were synthesized by using starch-PEI in which PEI act as reducing agent and starch as a stabilizer. Cytocompatibility studies carried out in C6 cells revealed that gold modification significantly improved the percentage cell viability even at higher polymer concentration.

First Report on High Entropy Alloy Nanoparticle Decorated Graphene.

This is the first report on synthesis of multimetal high entropy alloy (HEA) nanoparticle-few layer graphene composite. A two-step methodology for synthesizing multi-component HEA nanoparticle-graphene composite is provided. In the first step, high purity graphite powder was mechanically milled with metal powders (Ni, Cr, Co, Cu, Fe) to produce multimetal-graphite composite.

Efficacy of vinyl imidazole grafted cationized pullulan and dextran as gene delivery vectors: A comparative study.

The present study deals with efficacy evaluation of two cationised polysaccharides - Pullulan PEI and Dextran PEI as gene delivery vector after grafting with vinyl imidazole. Chemical modifications of these polysaccharides were confirmed by FTIR and NMR. Nanoplex formed from dextran PEI imidazole (DPI) were of smaller size and possess greater positive charge as compared to that of pullulan PEI imidazole (PPI).

Perceptions of Interns and General Medicine Examiners Regarding Cardiovascular Case Presentation in Practical Exams of General Medicine in Final MBBS Summative Examinations.

Introduction: Assessment of cardiovascular cases in practical exit exam is limited to simple history taking and physical examination. Standards of assessment are not uniform. This makes assessment less valid and reliable.