The Protein S Erlangen Mutation PROS1c.1904T>C (F635S) Suppresses Secretion.

Background: The recently identified PROS1 mutation Protein S Erlangen c.1904T>C, resulting in amino acid exchange F635S, is associated with severe quantitative protein S (PS) deficiency and clinical thrombosis. It was hypothesized that this deficiency is due to a secretion defect [1].

Museum DNA reveals a new, potentially extinct species of rinkhals (Serpentes: Elapidae: Hemachatus) from the Eastern Highlands of Zimbabwe.

Genetic information plays a pivotal role in species recognition and delimitation, but rare or extinct animals can be difficult to obtain genetic samples from. While natural history wet collections have proven invaluable in the description of novel species, the use of these historical samples in genetic studies has been greatly impeded by DNA degradation, especially because of formalin-fixation prior to preservation. Here, we use recently developed museum genomics approaches to determine the status of an isolated population of the elapid snake genus Hemachatus from Zimbabwe.

Protein S Erlangen: a novel PROS1 gene mutation associated with quantitative protein S deficiency.

The members of a Caucasian family were genetically analyzed on suspicion of hereditary protein S deficiency. A novel mutation, c.1904T>C, associated with severe quantitative protein S deficiency was found.

Complications and Radiographic Outcomes After Tibiotalocalcaneal Fusion With a Retrograde Intramedullary Nail.

Background: Tibiotalocalcaneal (TTC) arthrodesis is a salvage reconstructive procedure for ankle and subtalar arthritis and deformity. This study aims to identify fusion rates and complications of TTC arthrodesis procedures performed at our institution using a specific retrograde intramedullary nail. Additionally, we analyzed the subpopulation that sustained an intramedullary nail break.

Common Foot and Ankle Injuries: What Not to Miss and How Best to Manage.

Injuries to the foot and ankle are commonly encountered, especially among athletes, and can lead to morbidity if not managed appropriately. Health care professionals must have a firm understanding of injury anatomy, diagnoses, and management. This article provides a review of lateral talus process fractures, os trigonum injuries, Lisfranc injuries, turf toe, navicular stress fractures, and syndesmotic injuries.

Minimally invasive and open surgical treatment of proximal tibia fractures using a polyaxial locking plate system: a prospective multi-centre study.

Purpose: The purpose of this study was to determine whether a polyaxial locking plate of the latest generation (NCB PT(®), Zimmer Inc.) which can be applied both open and minimally invasively, can be used as a routine method of treatment for proximal tibia fractures.

Methods: Eighty-six patients (35 women, 51 men; mean age 51 years) were enrolled in this prospective multicentre trial.

Pharmacologic treatment of opioid-induced sedation in chronic pain.

Objective: To review the literature for pharmacologic management of opioid-induced sedation (OIS) in patients with chronic pain.

Data Sources: A search of MEDLINE (1966-October 2004) for English-language literature and selected bibliographies was completed. Search terms included pain, opioid, sedation, psychostimulants, amphetamines, modafinil, and donepezil.

Calcium as a branching signal in Neurospora crassa.

The divalent cation ionophore A23187 was found to induce apical branching in Neurospora crassa. Optimal effects were obtained by treatment with 0.1 mM ionophore for 30 min.

Purification and characterization of Neurospora crassa N-acetyl galactosaminoglycan deacetylase.

1. N-Acetyl galactosaminoglycan deacetylase was purified from Neurospora mycelium 215-fold in 25% yield to electrophoretic homogeneity. A single band corresponding to a molecular weight of 76,000 was obtained by sodium dodecyl sulfate/polyacrylamide gel electrophoresis.

An endogalactosaminidase from Streptomyces griseus.

An endogalactosaminidase has been purified 34-fold from the culture filtrate of Streptomyces griseus. This enzyme cleaves GalN-GalN linkages in oligogalactosaminoglycan, a galactosamine-rich oligosaccharide isolated from the culture filtrate of a Neurospora mutant. Since some or all of the GalN-GalN bonds in this molecule link positions 1 and 4, and are in the alpha-configuration, we are probably dealing with an endo-alpha-(1 leads to 4)-galactosaminidase, bu this characterization is only tentative because the few bonds cleaved by the enzyme could have a different structure.

Interaction of galactosaminoglycan with Neurospora conidia.

The inactivation of Neurospora crassa conidia by galactosaminoglycan isolated from cultures of this organism was followed by measuring colony-forming ability and ability to take up radiolabeled metabolites. When kinetic data on the loss of transport function and on killing were analyzed by use of target theory, it appeared that few "hits" are required for inactivation. However, studies with radio-labeled galactosaminoglycan mucopolysaccharides showed that cells receiving a single lethal hit have approximately 10(5) galactosaminoglycan molecules bound to them.

Mucopolysaccharide which regulates growth in Neurospora.

Neurospora produces a mucopolysaccharide (called MP) which inhibits its growth, causes vacuolation and agglutination of its cells, and precipitates its purified membrane protein. Cultures of a colonial strain display a phase of slow growth; the induction of this phase is traced to the production of MP by the mold. Stationary-phase cultures of wild type also produce MP.