Publications by authors named "Reinhard Fries"

Introduction: Ethiopia has a rapidly growing small ruminant sector, which faces low productivity due to husbandry practices and poor health condition of the animals. A study was conducted in Ethiopia's largest municipal abattoir with the objective to assess the health problems of sheep and goats presented for slaughter using standard ante-mortem and post-mortem methodology.

Methods: A cross-sectional study using systematic random sampling was conducted on 384 sheep and 384 goats from January to July 2014.

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The European Food Safety Authority (EFSA) identified extended-spectrum β-lactamase/AmpC β-lactamase (ESBL/AmpC)-producing as one of the main priority hazards for poultry. Different studies detected ESBL-producing at broiler fattening farms and in abattoirs, concluding that poultry meat is a potential source of human infection. Broiler breast skin samples taken in three abattoirs with different scalding techniques were examined for ESBL-producing (.

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Alternatives to conventional chicken meat and egg production are increasingly under discussion, especially because of the common practice of killing male day-old chicks from laying lines which has been banned from the beginning of 2022 in Germany and is planned to be banned during 2022 in other countries. Production of dual-purpose chicken lines is one possible solution, as such lines combine moderate laying and growth performance. The microbiological status of products from such breeds must be comparable to existing products on the market for food safety purposes.

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Article Synopsis
  • Researchers analyzed 23 isolates from Vietnamese slaughterhouses and markets in 2013, using various methods including whole-genome sequencing, to uncover the presence of ARGs and assess the resistance patterns.
  • Results indicated that 86.9% of samples were resistant to at least one antibiotic, with significant occurrences of multidrug resistance, and similar ARGs and plasmid profiles were found clustered among strains, suggesting potential spread along the pork supply chain.
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subspecies serovar Derby (. Derby) is one of the most frequent causes for salmonellosis in humans and animals. Understanding the genetic diversity of .

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We report the first draft genome sequence of a strain with plasmid-mediated resistance to colistin encoded by gene in Vietnam. serovar Rissen was isolated from a Vietnamese pig slaughterhouse in 2013. We can confirm that gene is identical to the first reported gene of the strain SHP45, isolated in 2015 from a Chinese pig.

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Antimicrobial resistance is a major health problem, particularly in developing countries like Bangladesh, where there is a paucity of information on resistance patterns and prevalence of antimicrobial determinants. Therefore, the aims of this study were to investigate the prevalence of resistance, including multi-drug resistance (MDR), and the associated genetic determinants in isolates from cloacal swabs of live broiler chickens in Bangladesh. Altogether, 400 cloacal swabs (200 from Rajshahi and 200 from Dhaka divisions) were randomly collected from individual chickens in 50 broiler farms.

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Saintpaul (SSa) is increasingly reported from food and foodborne outbreak cases. Pulsed field gel electrophoresis (PFGE) is used for screening and tracking of infections. Widespread use of antimicrobial agents in humans and food animals could result in antimicrobial resistant serotypes.

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A cross-sectional investigation was conducted concerning prevalence, antimicrobial resistance, multidrug resistance patterns, and serovar diversity of Salmonella in chicken meat sold at retail in Yangon, Myanmar. The 141 chicken meat samples were collected at 141 retail markets in the Yangon Region, Myanmar, 1 November 2014 to 31 March 2015. Information on hygienic practices (potential risk factors) was retrieved via checklists.

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Pork safety is an important public health concern in Vietnam and is a shared responsibility among many actors along the pork value chain. We examined the knowledge, perceptions, and practices regarding food safety, disease, and health risk among selected pork value chain actors (slaughterhouse owners and workers, people living around slaughterhouses, pork sellers, consumers, and veterinary and public health staff) in three districts in Hung Yen Province, Vietnam. We randomly selected 52 pork value chain actors to be surveyed through questionnaires, observation checklists, key informant interviews, and focus group discussions.

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Introduction: Salmonella has been reported from foods and the food production environment, with outbreaks occurring in the human population worldwide.

Methodology: A survey on Salmonella in two beef production lines (a beef abattoir line and a processing line) in Addis Ababa, Ethiopia was conducted, with a total of 668 various samples randomly collected from animal-related materials, the environment, and a beef product (mortadella).

Results: Overall, a 12.

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Previous research on trichinellosis in Africa focused on isolating Trichinella from wildlife while the role of domestic pigs has remained highly under-researched. Pig keeping in Uganda is historically recent, and evidence on zoonotic pig diseases, including infection with Trichinella species, is scarce. A cross-sectional survey on Trichinella seroprevalence in pigs was conducted in three districts in Central and Eastern Uganda from April 2013 to January 2015.

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Toxigenic Escherichia coli (E. coli) are an important cause of gastroenteritis in developing countries. In Ethiopia, gastroenteritis due to food-borne disease is a leading cause of death.

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During the catching of broilers for slaughter, 2 to 3 birds are grabbed per hand at one leg at the same time. From an animal welfare point of view, this procedure is under critical observation from animal welfare administration and the general public.In this paper 2 catching methods were compared: the routinely used 1-leg catching method, and a second tech-nique whereby birds were grabbed by both legs with a maximum of 2 birds per hand (2-leg catching method).

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Processing changes the meat ecosystem and, hence, has an impact on the microbiological load and quality of the product. Microbial contamination decreases shelf life and, if a pathogen is present, increases the risk of zoonoses in humans. The aim of this study was to assess the microbiological load, including zoonotic agents, in ready-to-eat beef mortadella from Ethiopian supermarkets.

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This study was conducted to determine the Campylobacter contamination rate of chicken carcasses and the processing lines of modern processing plants in Malaysia. Three hundred sixty samples were collected from 24 flocks of broiler chickens at 12 modern poultry processing plants in 6 states of Malaysia. Fresh fecal droppings were collected from crates in the arrival area.

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A total of 360 samples including fresh fecal droppings, neck skins, and swab samples was collected from 24 broiler flocks and processed by 12 modern processing plants in 6 states in Malaysia. Ninety samples from 10 traditional wet markets located in the same states as modern processing plants were also collected. Microbiological isolation for Campylobacter was performed following ISO 10272-1:2006 (E).

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The occurrence of Salmonella in food of animal origin in Chiang Mai province was investigated by using a cross-sectional study during several phases of the pork production chain (cutting, transport, and retail) and of the environment in the cutting unit of a slaughterhouse. In total, 173 pork samples were obtained during the cutting phase, 173 samples from transported pork, 200 samples from retail products, and 300 samples from the slaughterhouse environment. Salmonella was detected in 55.

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The effect of different CO2-stunning on the corneal reflex response and blood parameters in (arterio-venous) killing blood was investigated in n = 614 slaughter pigs (carcass weight = 92-94 kg) at two different abattoirs. CO2-anaesthesia lead to remarkable decrease of PO2 (hypoxaemia), increase of PCO2 (hypercapnia), decrease of pH (respiratory acidosis) and increase of [strong ion difference = SID3] (basic reaction caused by electrolytes). Pigs with subclinical post mortem obtained lung lesions showed no significant modified value of the reflex answer or changes in parameters of killing blood compared to the animals with healthy lungs.

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The examination of a total of 180 samples from both captured and cultured shrimps from Sri Lanka for the presence of Salmonella revealed an overall prevalence of 12.8%. The prevalence of Salmonella in captured shrimps and cultured shrimps was 14.

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To obtain a more detailed understanding of the prevalence of classical scrapie infections in a heavily affected German sheep flock (composed of 603 sheep and 6 goats), we analysed 169 sheep and 6 goats that carried the genotypes susceptible to the disease and that were therefore culled following discovery of the index case. The initial tests were performed using the Biorad TeSeE ELISA and reactive results were verified by official confirmatory methods (OIE-immunoblot and/or immunohistochemistry (IHC)) to demonstrate the deposition of scrapie-associated PrP(Sc) in the brain stem (obex). This approach led to the discovery of 40 additional subclinically scrapie-infected sheep.

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A cross-sectional study was conducted to determine microbial contamination of pig carcasses at a slaughterhouse in Vientiane, capital of Lao People Democratic Republic (Lao PDR). Between November 2004 and April 2005, 62 pig carcasses were randomly selected. From each carcass, pooled swabs (from "1" prior to and "2" after evisceration) and 25 g of tissue of mesenteric lymph nodes (MLN) were collected.

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Infection with Salmonella (S.) is the most frequently reported cause of bacterial food-borne illness worldwide. Poultry are a common source and, in recent years, much attention has been focused in determining the prevalence of Salmonella during the different stages in the poultry production chain.

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Three pig abattoirs in northeastern Germany were visited for examination of flexible plastic doors at the access to the chilling room. Swabs were taken from the front and back of the doors and were examined for aerobic plate count, Enterobacteriaceae, Salmonella, and Campylobacter. The aerobic plate count was within the range of 1.

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Air sacculitis is a common lesion found in herds during poultry meat inspection. To support the decision during inspection, 101 carcasses from turkey herds with frequently occurring air sacculitis (unfit for human consumption because of air sacculitis) were examined and 96 E. coil-isolates were collected.

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