Exome Sequencing of 5 Families with Severe Early-Onset Periodontitis.

Periodontitis is characterized by alveolar bone loss leading to tooth loss. A small proportion of patients develop severe periodontitis at the juvenile or adolescent age without exposure to the main risk factors of the disease. It is considered that these cases carry rare variants with large causal effects, but the specific variants are largely unknown.

Altered macrophagic THP-1 cell phagocytosis and migration in bisphosphonate-related osteonecrosis of the jaw (BRONJ).

Objectives: Local immune dysfunction via macrophages is a proposed aetiology of bisphosphonate-related osteonecrosis of the jaw (BRONJ). This study aimed to clarify the effects of various bisphosphonates on macrophage function using a THP-1 monocytic model to examine migration, phagocytosis, and fibrin structure.

Materials And Methods: THP-1 cell migration was measured in the presence and absence of zoledronate, ibandronate, risedronate, alendronate, pamidronate (0.

Resin-based pit-and-fissure sealants: microleakage reduction and infiltration enhancement using a bonding agent.

Purpose: To evaluate the marginal microleakage and the infiltration ability of pit-and-fissure sealants by applying the conventional sealing technique in comparison to using an additional bonding agent.

Materials And Methods: Extracted non-carious permanent molars (n = 60) were first stored in sterile saline solution and then assigned to one of two groups: group C (control) was sealed (Helioseal F) by using the conventional technique, while in group BA (bonding agent), a bonding agent (OptiBond FL) was additionally applied prior to sealing. The teeth were thermocycled (1000 cycles, 5°C to 55°C, dwell time 30 s), then varnished and immersed in 5% methylene blue solution for 24 h.

IGF-independent effects of IGFBP-2 on the human breast cancer cell line Hs578T.

There is evidence that insulin-like growth factor-binding protein (IGFBP-2), a modulator of the actions of IGFs, also has IGF-independent effects in human tumor cell lines. These involve specific binding of IGFBP-2 to alpha5beta1-integrin, followed by alterations in the phosphorylation status of downstream signaling molecules. Previously, IGFBP-2 has also been shown to be associated with cell proliferation, adhesion and migration.

IGFs, IGFBPs, IGF-binding sites and biochemical markers of bone metabolism during differentiation in human pulp fibroblasts.

Objective: To investigate the role of the insulin-like growth factors (IGF) system during the differentiation of human pulp-derived fibroblasts (HPF).

Methods: Primary HPF were cultured for 24 days in DMEM medium with IGF-I or IGF-II (50 ng/ml each). Cell growth and morphology, alkaline phosphatase (ALP) activity, the concentration of free deoxypyridinoline (DPD), IGF-I, -II, IGFBP-2 and -3 were studied.