Transcriptomic and in silico analysis of BLACE (B-cell acute lymphoblastic leukemia expressed), a new non-coding RNA, as a diagnostic biomarker in B-cell ALL.

ALL (acute lymphoblastic leukemia) is a type of hematological malignancy that involves developmental and differentiation arrest at the lymphoblast stage. BLACE, a gene specifically expressed in B-cell acute lymphoblastic leukemia shows little or no expression in mature B-lymphocytes. The current pilot study involves transcriptional analysis of BLACE in B-cell ALL patients.

Comparing dual oral agents plus insulin vs. Triple oral agents in uncontrolled type II diabetes: A pilot study.

Introduction: Type II Diabetes mellitus (T2DM) patients often do not achieve glycemic control with oral hypoglycemic agents (OHAs). There are two main approaches to address this challenge: transitioning to a triple OHA regimen, or adding Insulin to the existing dual OHA regimen.

Aim: This study aimed to compare the efficacy of adding Insulin to dual OHAs (Sitagliptin + Metformin) against adding a third OHA to Sitagliptin + Metformin in achieving glycemic control among patients with uncontrolled T2DM.

Synthesis and biological evaluation of diclofenac acid derivatives as potential lipoxygenase and α-glucosidase inhibitors.

Inflammation is a complex physiological response associated with the onset and progression of various disorders, including diabetes. In this study, we synthesized a series of diclofenac acid derivatives and evaluated their potential anti-diabetic and anti-inflammatory activities. The compounds were specifically assessed for their ability to inhibit 15-lipoxygenase (15-LOX) and α-glucosidase enzymes.

In-silico studies on evaluating the liver-protective effectiveness of a polyherbal formulation in preventing hepatocellular carcinoma progression.

Unlabelled: Liv-52, an herbal formulation consisting of seven distinct plants and Mandur Bhasma, is recognized for its hepatoprotective, anti-inflammatory, and antioxidant properties. To investigate the pharmacological potential of each phytochemical from these plants, we conducted ADMET analysis, molecular docking, and molecular dynamic simulations to identify potent molecules capable of inhibiting the interaction between Alpha-fetoprotein (AFP) and Cysteine aspartyl protease 3 (Caspase-3/CASP3). In our study, we have used molecular docking of all the compounds against AFP and filtered them on the basis of ADME properties.

Nordic environmental resilience: balancing air quality and energy efficiency by applying artificial neural network.

Maintaining public health and environmental safety in the Nordic nations calls for a strict plan to define exact benchmarks on air quality and energy efficiency. This study investigates the complicated interaction of decentralized energy production (DEP) with energy efficiency, and air quality index in the Nordic nations from 1990 to 2022 using System GMM and Artificial Neural Network (ANN) approach. Our research explored positive role of decentralized energy production and technological advancement to propel notable increases in energy efficiency, hence lowering pollution expressed as PM2.

Understanding childhood obesity in Pakistan: exploring the knowledge, attitudes, practices of mothers, and influential factors. A cross-sectional study.

Background: Childhood obesity is a significant public health issue with far-reaching implications. The World Health Organization reported that in 2020, around 38 million children under five were overweight or obese globally, and in 2016, 340 million children and adolescents aged 5-19 were affected. In Pakistan, the situation is alarming; 66.

A novel homozygous missense variant in causes cone dystrophy in a consanguineous Pakistani family.

Background: Cone dystrophy is a heterogeneous hereditary retinal disorder with disease symptoms appearing in the late first or early second decades of life.

Methods: A consanguineous Pakistani family with three affected individuals underwent detailed clinical and genetic investigation.

Results: The proband, a 63-years old male, showed severely reduced day vision, a visual acuity of counting fingers (CF), color vision deficiency, high myopia and photophobia.

A Rhodamine-based high-sensitivity low-cytotoxicity probe for rapid turn-on detection of Hg.

By integrating Rhodamine B and 4-phenylmorpholine moieties, a novel fluorescent probe named RhPy is synthesized for detecting Hg. Its recognition mechanism involves the reaction of Hg with dithiooxamide, ultimately triggering the opening of the Rhodamine spirolactam and forming a new molecule RhPy-S with strong emission. The probe exhibits impressive limit of detection (0.

Molecular modeling and synthesis of novel benzimidazole-derived thiazolidinone bearing chalcone derivatives: a promising approach to develop potential anti-diabetic agents.

Diabetes mellitus (DM) is a disorder which is raised at the alarming level and it is characterized by the hyperglycemia results from the impaired action of insulin, production of insulin or both of these simultaneously. Consequently, it causes problems or failure of different body organs such as kidneys, heart, eyes, nerve system. Since this disease cannot be completely cured until now, we aimed to design series of enzymes inhibitors and tested them for DM treatment.

Vagal stimulation ameliorates murine colitis by regulating SUMOylation.

Inflammatory bowel diseases (IBDs) are chronic debilitating conditions without cure, the etiologies of which are unknown, that shorten the lifespans of 7 million patients worldwide by nearly 10%. Here, we found that decreased autonomic parasympathetic tone resulted in increased IBD susceptibility and mortality in mouse models of disease. Conversely, vagal stimulation restored neuromodulation and ameliorated colitis by inhibiting the posttranslational modification SUMOylation through a mechanism independent of the canonical interleukin-10/α7 nicotinic cholinergic vagal pathway.

Molecular Analysis of Genes CEBPA, NPM1, IDH1, and RUNX1 Polymorphisms as Biomarker Potential in Leukemia Patients.

Leukemia is found in approximately 2.3 million people worldwide and causes many deaths all over the world. This research study was conducted to figure out the link of single nucleotide polymorphisms of genes CEBPA (rs34529039), NPM1 (rs753788683), IDH1 (of rs11554137) and RUNX1 (rs13051066) polymorphisms as biomarker potential in leukemia patients.

Alginate-like exopolysaccharides extracted from different waste sludges exhibit varying physicochemical and material properties.

This study examined the influence of different wastewater treatment processes on the physicochemical properties of Alginate-Like Exopolymers (ALE) extracted from waste sludge. Sludge samples were collected from wastewater treatment plants (WWTPs) processing both combined industrial and domestic wastewater, as well as domestic wastewater alone. Among the processes studied, aerobic granular sludge (AGS) produced the highest ALE yield (352 ± 50 mg/g-VS), significantly exceeding that from membrane bioreactor (170 ± 41 mg/g-VS) and conventional activated sludge (<130 mg/g-VS).

Risk Stratification for Autoimmune Hemolytic Anemia in Children With Pediatric Transfusion-Dependent Thalassemia.

Objective This study aimed to identify the risk factors for autoimmune hemolytic anemia (AIHA) in children with transfusion-dependent thalassemia (TDT). Methodology This cross-sectional study was conducted at the Department of Child Health, Khyber Teaching Hospital, Peshawar, Pakistan, from March 25, 2024, to September 25, 2024. A total of 300 patients under 18 years of age, diagnosed with TDT, were included in the study.

Novel interfaces for internet of wearable electrochemical sensors.

The integration of wearable devices, the Internet of Things (IoT), and advanced sensing platforms implies a significant paradigm shift in technological innovations and human interactions. The IoT technology allows continuous monitoring in real time. Thus, Internet of Wearables has made remarkable strides, especially in the field of medical monitoring.

Corrigendum: Prevalence, pattern, and determinants of disabilities in India: Insights from NFHS-5 (2019-21).

[This corrects the article DOI: 10.3389/fpubh.2023.

Key predictors of fertility: Exploring the role of Vitamin-D.

Background & Objectives: The relationship of Vitamin-D (VD) with Luteinizing Hormone (LH), Follicle stimulating Hormone (FSH) and Anti Mullerian Hormone (AMH) is recognized. This study was conducted to determine whether VD is a predictor of fertility and investigate its relationship with female reproductive hormones and markers of ovarian reserve in female population of Karachi.

Methods: A cross-sectional study was performed from July 2020 to June 2022 at the Aga Khan University Hospital (AKUH) with recruitment of 135 fertile and infertile subjects.

Impact and outcomes of hyperglycemia among post-operative children who underwent repair of congenital cardiac defects in a tertiary care setup of Pakistan.

Objective: To determine frequency of postoperative hyperglycemia in children who underwent repair of Congenital Cardiac Defects (CCDs) in a tertiary care hospital of Pakistan. The other objective was to compare outcomes in children with versus without postoperative hyperglycemia who underwent repair of CCDs in a tertiary care hospital of Pakistan.

Methods: A descriptive study was conducted at the Cardiac Intensive Care Unit (CICU), Aga Khan University Hospital (AKUH), Karachi, Pakistan from October 3, 2020, to April 2, 2021.

Racial Disparities in Immediate Breast Reconstruction after Mastectomy: A Systematic Review and Meta-Analysis.

In the past few decades, there has been a gradual increase in breast reconstruction post mastectomy; however, there exists a conflict about whether race has an influence on reconstruction rates. We conducted an electronic search from MEDLINE and Cochrane CENTRAL from their inception to September 2022. Primary outcome was disparity in rates of Immediate Breast Reconstruction (IBR) in racial minorities.

A comparative study on utilization of different plant-derived nano-mucilage as a fat replacer in yogurt: Product optimization, physicochemical attributes, shelf-life evaluation, and consumer perception with market orientation.

This study aimed to utilize different plant-derived mucilage as a fat substitute in yogurt production. rhizome mucilage (CEM), fruit mucilage (CDM), and husk mucilage (PHM) were extracted using different extraction process, and spray dried to acquire nano-scaled mucilage particles (100-300 nm). Seven different types of yogurts were prepared with the addition of varied mucilage concentrations (1-10 % /).

The Impact of Intra-abdominal Pressure on Perioperative Outcomes in Laparoscopic Cholecystectomy.

Introduction: Intra-abdominal pressure (IAP) is a critical factor in laparoscopic cholecystectomy, potentially affecting various perioperative outcomes. Understanding the impact of different IAP levels can guide clinical practice to optimize patient safety and recovery.

Objective: This study aimed to evaluate the effects of low, moderate, and high IAPs on perioperative outcomes in patients undergoing laparoscopic cholecystectomy.