Discovery of anti-Mycobacterium tuberculosis desertomycins from Streptomyces flavofungini TRM90047 based on genome mining and HSQC-TOCSY.

Tuberculosis caused by Mycobacterium tuberculosis (M. tb) is a major public health problem with high morbidity and mortality worldwide. In our previous study, we found that a fermentation product of Streptomyces flavofungini TRM90047 exhibited anti-M.

Iron gallic acid biomimetic nanoparticles for targeted magnetic resonance imaging.

Developing T1-weighted magnetic resonance imaging (MRI) contrast agents with enhanced biocompatibility and targeting capabilities is crucial owing to concerns over current agents' potential toxicity and suboptimal performance. Drawing inspiration from "biomimetic camouflage," we isolated cell membranes (CMs) from human glioblastoma (T98G) cell lines via the extrusion method to facilitate homotypic glioma targeting. At an 8:1 mass ratio of ferric chloride hexahydrate to gallic acid (GA), the resulting iron (Fe)-GA nanoparticles (NPs) proved effective as a T1-weighted MRI contrast agent.

The Role of Rv1476 in Regulating Stress Response and Intracellular Survival of .

The virulence of () is related to many factors, including intracellular survival, cell wall permeability, and cell envelope proteins. However, the biological function of the membrane protein remains unclear. To investigate the potential role played by , we constructed an overexpression strain and found that overexpression of enhanced the intracellular survival of , while having no impact on the growth rate in vitro.

Transcriptomic analysis of the hypoxia-inducible factor 1α impact on the gene expression profile of chicken fibroblasts under hypoxia.

Hypoxia-inducible factor 1 (HIF-1) is a transcriptional regulator that mediates cellular adaptive responses to hypoxia. Hypoxia-inducible factor 1α (HIF-1α) is involved in the development of ascites syndrome (AS) in broiler chickens. Therefore, studying the effect of HIF-1α on the cellular transcriptome under hypoxic conditions will help to better understand the mechanism of HIF-1α in the development of AS in broilers.

Precise evaluation of batch adsorption kinetics of plant total polyphenols based on a flow-injection online spectrophotometric method.

Efficient evaluation of adsorption kinetics of plant total polyphenols is essential for the design of adsorption separation of bioactive compounds. The conventional method uses manual sampling with poor reproducibility. Here, we developed a new method for on-line determination of total polyphenol content (TPC) in plant extracts by applying the Folin-Ciocalteu method in flow-injection analysis (FIA).

Epinephrine Stimulates Growth and Biofilm Formation.

The human stress hormones catecholamines play a critical role in communication between human microbiota and their hosts and influence the outcomes of bacterial infections. However, it is unclear how senses and responds to certain types of human stress hormones. In this study, we screened several human catecholamine stress hormones (epinephrine, norepinephrine, and dopamine) for their effects on growth.

Dual responsive self-healing hydrogels with wide stability and excellent mechanical strength based on aliphatic polycarbonate.

The wide development of hydrogels had been used in many filed due to the high water-containing and tough three-dimensional structure, however, the poor mechanical and multi-functional properties of hydrogel can be limited in its applications deeply. Herein, the dual responsive self-healing hydrogels with tough mechanical properties were manufactured by dual-physical cross-linking based on biodegradable aliphatic polycarbonate. Choosing the soft and hard segments to design the polymeric hydrogel not only can facilitate the dual-dynamic bonding interactions but also the resilient hydrogels possess robust and controllable mechanical strength (6.

Construction of nano-drug delivery and antitumor system of stimuli-responsive polypeptides.

The size of the nanoparticles is moderate and the dispersion is well, which will not be recognized nonspecifically and clearance by the endothelial reticular system. In this study, stimuli-responsive polypeptides nano-delivery system has been constructed, which can realize the response to various stimuli in the tumor microenvironment. Tertiary amine groups are grafted to the side chain of polypeptides as the point of charge reversal and particle expansion.

Sustained-release behavior and the antitumor effect of charge-convertible poly(amino acid)s drug-loaded nanoparticles.

Enhancing tissue permeability and achieving drug aggregation is the key to targeted tumor therapy. A series triblock copolymers of poly(ethylene glycol)-poly(L-lysine)-poly(L-glutamine) were synthesized by ring-opening polymerization, and charge-convertible nano-delivery system was constructed by loading doxorubicin (DOX) with 2-(hexaethylimide) ethanol on side chain. In normal environment (pH = 7.

Construction of poly(amino acid)s nano-delivery system and sustained release with redox-responsive.

A series of novel poly(amino acid)s materials were designed to prepare drug-loaded nanoparticles by physical encapsulation and chemical bonding. The side chain of the polymer contains a large number of amino groups, which effectively increases the loading rate of doxorubicin (DOX). The structure contains disulfide bonds that showing a strong response to the redox environment, which can achieve targeted drug release in the tumor microenvironment.

Optimizing the Measurement of 0.5-kHz Cubic Distortion Product Otoacoustic Emission.

Background: The measurement of low-frequency cubic distortion product otoacoustic emission, for example, 0.5-kHz cubic distortion product otoacoustic emission, is often severely affected by background noise, and currently 0.5-kHz cubic distortion product otoacoustic emission is not commonly applicable in clinical setting.

Detection of Phosphatidylethanol in Whole Blood by UPLC-MS/MS.

Objectives: To establish the ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to detect ethanol metabolites phosphatidylethanol (PEth) in whole blood.

Methods: An appropriate amount of aqueous solution including 1% formic acid was added to 100 μL whole blood, the protein was precipitated with acetone, centrifuged and the supernatant was purified and enriched by using Bond Elut Certify column. The eluent was redissolved with 1/1 isopropanol/acetonitrile (/) solution after nitrogen blowing and then tested by UPLC-MS/MS.

Screening of Microbial Fermentation Products for Anti- Activity.

Tuberculosis (TB), caused by , is the leading infectious cause of mortality worldwide. The emergence of drug-resistant has made the control of TB more difficult. In our study, we investigated the ability of microorganism fermentation products from the soil to inhibit .

The Activity of Plant-Derived Ren's Oligopeptides-1 against the Pseudorabies Virus.

Newly synthesized Ren's oligopeptides-1 was found to have an antiviral effect in clinical trials, and the purpose of this study was to further demonstrate the antiviral activity of Ren's oligopeptides-1 against the PRV 152-GFP strain. We used the real-time cell analysis system (RTCA) to detect the cytotoxicity of different concentrations of Ren's oligopeptides-1. We then applied high content screening (HCS) to detect the antiviral activity of Ren's oligopeptides-1 against PRV.

Distinct Persistence Fate of Mycobacterium tuberculosis in Various Types of Cells.

Mycobacterium tuberculosis can invade different cells with distinct persistence fates because cells are equipped with different host restriction factors. However, the underlying mechanisms remain elusive. Here, we infected THP1 and Raw264.

Corn bracts loading copper sulfide for rapid adsorption of Hg(II) and sequential efficient reuse as a photocatalyst.

Hg(II) ions in wastewater are highly toxic to the environment and human health, yet many materials to remove the ions exhibit lower adsorption efficiency, and few studies report the reuse of Hg(II)-loaded waste materials. Here, a cheap and efficient adsorbent was prepared for the removal of Hg(II) based on corn bracts (CB) loading copper sulfide (CuS), and the Hg(II)-adsorbed material was reused as a photocatalyst. By changing the adsorption variables such as pH, adsorbent dosage, Hg(II) concentration, contact time and coexisting ions, the optimum adsorption conditions were obtained.

The Genome Analysis of YC-XJ1 with Diverse Xenobiotics Biodegrading Capacity and Degradation Characteristics of Related Hydrolase.

YC-XJ1 isolated from desert soil exhibited a diverse degrading ability towards aromatic oxyphenoxypropionic acid esters (AOPPs) herbicide, phthalate esters (PAEs), organophosphorus flame retardants (OPFRs), chlorpyrifos and phoxim. The genome of YC-XJ1 was sequenced and analyzed systematically. YC-XJ1 contained a large number of exogenous compounds degradation pathways and hydrolase resources.

Light-driven topochemical polymerization under organogel conditions of a symmetrical dipeptide-diacetylene system.

A symmetrical dipeptide-based diacetylene system (DAs) was found to be able to self-assemble in dichloromethane and to form a compact fiber network which resulted in a stable organogel. As a consequence of the organogel formation, we explored the possibility to run a light-induced topochemical polymerization. This is a typical reaction of ordered diacetylene moieties taking advantage from their organized packing mode resulting from fiber formation.

Effect of melatonin on monochromatic light-induced T-lymphocyte proliferation in the thymus of chickens.

A total of 360 post-hatching day 0 (P0) Arbor Acre male broilers, including intact, sham operation and pinealectomy groups, were exposed to white light (WL), red light (RL), green light (GL) and blue light (BL) from a light-emitting diode (LED) system until for P14. We studied the effects of melatonin and its receptors on monochromatic light-induced T-lymphocyte proliferation in the thymus of broilers. The density of proliferating cell nuclear antigen (PCNA) cells and the proliferation of T-lymphocytes in response to Concanavalin A (ConA) in GL significantly increased both in vivo and in vitro (from 9.

Gas chromatography/mass spectrometry determination of amphetamine-related drugs and ephedrines in plasma, urine and hair samples after derivatization with 2,2,2-trichloroethyl chloroformate.

A new analytical approach, based on derivatization with 2,2,2-trichloroethyl chloroformate and gas chromatography/mass spectrometry (GC/MS), was investigated for qualitative and quantitative analyses of a large range of amphetamine-related drugs and ephedrines in plasma, urine and hair samples. Sample preparation involved alkaline extraction of analytes from biological samples using Extrelut columns, after addition of the internal standard 3,4-methylenedioxypropylamphetamine (MDPA), and subsequent derivatization to produce 2,2,2-trichloroethylcarbamates. GC/MS analyses, in splitless mode using a slightly polar 30-m capillary column, were performed with quadrupole or ion trap instruments.