Microbial Growth, Survival, and Cryotolerance in Plant-Based Milk Alternatives: A Study with .

The increasing popularity of plant-based milk products as an alternative to traditional bovine milk has sparked concerns about their safety and nutritional impact. This study focuses on the growth, survival, and cryotolerance behavior of (strains: ATCC 19115 and RS1) in various plant-based milk substitutes. Samples of almond milk, oat milk, soy milk, and bovine milk, all subjected to ultra-high temperature treatment, were evaluated for their influence on growth at 4°C and survival through repeated freezing and thawing cycles.

Enhancing gluten detection assay development through optimization of gliadin extraction conditions.

Gluten consumption can lead to severe health conditions in certain individuals, and following a strict gluten-free diet is often the only effective treatment option. Therefore, it is crucial to develop a gluten detection method that is accurate, sensitive, and specific to ensure the absence of gluten. An important aspect of developing effective gluten detection tests is the implementation of an efficient gluten extraction method.

Generation of a Beta-Cell Transplant Animal Model of Diabetes Using CRISPR Technology.

Since insulin deficiency results from pancreatic beta-cell destruction, all type 1 and most type 2 diabetes patients eventually require life-long insulin injections. Insulin gene synthesis could also be impaired due to insulin gene mutations as observed in diabetic patients with MODY 10. At this point, insulin gene therapy could be very effective to recompense insulin deficiency under these circumstances.

Cell Death in Escherichia coli: Incomplete Base Excision Repair under Depletion of DapB and Dxr Proteins.

The generation of reactive oxygen species (ROS) within the cell is a significantly shared aspect of bacterial cell death against different stress conditions. The main cell death mechanism due to the generation of reactive oxygen species is then the incomplete base excision repair (BER) in response to oxidized nucleotides. In their recent article in , C.

The Recent Original Perspectives on Nonculture-Based Bacteria Detection Methods: A Comprehensive Review.

Foodborne diseases that are primarily caused by pathogenic bacteria are of major public health concern globally. One of the key strategies in minimizing and controlling the risk of contamination of food with such pathogens requires establishing effective detection and tracking methods of zoonotic bacteria. Although culture-based pathogen detection methods are developed and widely used by the industry traditionally, nonculture-based zoonotic bacteria detection methods are now more widely investigated and used owing to the recent developments in nucleic acid and immunological-based detection methods.

Mutant construction and integration vector-mediated gene complementation in Listeria monocytogenes.

Genes that play role in stress response mechanisms and other phenotypes of bacteria can be identified by construction and screening of mutant libraries. In this chapter, we describe the construction and screening of mutant libraries of Listeria monocytogenes using a plasmid, pMC38, carrying a mariner-based transposon system (TC1/mariner) and constructed by Cao et al. (Appl Environ Microbiol 73:2758-2761, 2007).

Bovine Staphylococcus aureus: dose response to iodine and chlorhexidine and effect of iodine challenge on antibiotic susceptibility.

Staphylococcus aureus is a gram-positive organism that is frequently associated with clinical or subclinical mastitis. The use of germicidal teat dips is one of the measures taken by the dairy industry to control mastitis. Iodine and chlorhexidine compounds are commonly used disinfectants in teat dips.

Temperature-dependent requirement for catalase in aerobic growth of Listeria monocytogenes F2365.

Listeria monocytogenes is a Gram-positive, psychrotrophic, facultative intracellular food-borne pathogen responsible for severe illness (listeriosis). The bacteria can grow in a wide range of temperatures (1 to 45°C), and low-temperature growth contributes to the food safety hazards associated with contamination of ready-to-eat foods with this pathogen. To assess the impact of oxidative stress responses on the ability of L.

Impact of growth temperature and agar versus liquid media on freeze-thaw tolerance of Yersinia enterocolitica.

Yersinia enterocolitica is a foodborne pathogen well known for its ability to grow at low temperatures. Recent studies with another psychrotrophic foodborne pathogen, Listeria monocytogenes, revealed that temperature of growth had pronounced impact on survival following repeated freezing and thawing (cryotolerance). Listerial cryotolerance was significantly more pronounced when bacteria were grown at 37 degrees C than following growth at either 4 degrees C or 25 degrees C.

Impact of antibiotic stress on acid and heat tolerance and virulence factor expression of Escherichia coli O157:H7.

This study was conducted to determine the effect of antibiotic stress on the virulence factor expression, simulated gastric fluid (SGF; pH 1.5) survival, and heat tolerance (56 degrees C) of Escherichia coli O157:H7. The MIC for three antibiotics (trimethoprim, ampicillin, and ofloxacin) was determined for two E.