Publications by authors named "Reginald V C T van der Kwast"
Article Synopsis
- N-6-methyladenosine (m6A) is a key RNA modification in eukaryotic cells that can be dynamically regulated by specific enzymes, influencing mRNA stability and function.
- Changes in m6A levels, especially in the context of ischemia and hypoxia, affect heart failure by altering mRNA processes like splicing and translation.
- The study identified that hypoxia increases m6A levels in vasoactive microRNAs in fibroblasts, with the enzyme METTL4 playing a major role in this modification, impacting the suppression of target mRNAs.
Adenosine-to-inosine (A-to-I) editing in the seed sequence of microRNAs can shift the microRNAs' targetomes and thus their function. Using public RNA-sequencing data, we identified 35 vasoactive microRNAs that are A-to-I edited. We quantified A-to-I editing of the primary (pri-)microRNAs in vascular fibroblasts and endothelial cells.
View Article and Find Full Text PDFTherapeutic neovascularization can facilitate blood flow recovery in patients with ischemic cardiovascular disease, the leading cause of death worldwide. Neovascularization encompasses both angiogenesis, the sprouting of new capillaries from existing vessels, and arteriogenesis, the maturation of preexisting collateral arterioles into fully functional arteries. Both angiogenesis and arteriogenesis are highly multifactorial processes that require a multifactorial regulator to be stimulated simultaneously.
View Article and Find Full Text PDFMicroRNAs are posttranscriptional regulators of gene expression. As microRNAs can target many genes simultaneously, microRNAs can regulate complex multifactorial processes, including post-ischemic neovascularization, a major recovery pathway in cardiovascular disease. MicroRNAs select their target mRNAs via full complementary binding with their seed sequence, i.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates the role of 14q32 small nucleolar RNAs (snoRNAs) in cardiovascular disease, finding they operate independently of previously studied microRNAs in this region.
- Researchers found significant associations between specific single nucleotide polymorphisms (SNPs) in snoRNA clusters and heart failure in a large participant group, suggesting snoRNAs modify cardiovascular disease risk.
- Expression levels of 14q32 snoRNAs were highly vessel-specific, notably up-regulated in failing heart tissues and post-heart attack patients, indicating their importance in cardiovascular pathology.
Rationale: Adenosine-to-inosine editing of microRNAs has the potential to cause a shift in target site selection. 2'-O-ribose-methylation of adenosine residues, however, has been shown to inhibit adenosine-to-inosine editing.
Objective: To investigate whether angiomiR miR487b is subject to adenosine-to-inosine editing or 2'-O-ribose-methylation during neovascularization.