Micro-flow size-exclusion chromatography for enhanced native mass spectrometry of proteins and protein complexes.

Size-exclusion chromatography (SEC) employing aqueous mobile phases with volatile salts at neutral pH combined with native mass spectrometry (nMS) is a valuable tool to characterize proteins and protein aggregates in their native state. However, the liquid-phase conditions (high salt concentrations) frequently used in SEC-nMS hinder the analysis of labile protein complexes in the gas phase, necessitating higher desolvation-gas flow and source temperature, leading to protein fragmentation/dissociation. To overcome this issue, we investigated narrow SEC columns (1.

Meeting report: 8(th) HIC/RPC Bioseparation Conference.

The 8(th) HIC/RPC Bioseparation Conference was held from March 3-7, 2013 at the Westin Savannah Harbor Resort in Savannah, Georgia, USA. Read this meeting report to find out more on the latest developments in chromatography for bioprocess applications.