Cytotoxic effect of protic ionic liquids in HepG2 and HaCat human cells: and studies.

Protic ionic liquids (PILs) are innovative chemical compounds, which due to their peculiar nature and amazing physico-chemical properties, have been studied as potential sustainable solvents in many areas of modern science, such as in the industrial fields of textile dyeing, pharmaceuticals, biotechnology, energy and many others. Due to their more than probable large-scale use in a short space of time, a wider analysis in terms of ecotoxicity and biological safety to humans has been attracting significant attention, once many ionic liquids were found to be "a little less than green compounds" towards cells and living organisms. The aim of this study is to investigate the cytotoxicity of 13 recently synthesized PILs, as well as to reinforce knowledge in terms of key thermodynamic magnitudes.

Comparison between the polymerase chain reaction-based screening and the Southern blot methods for identification of fragile X syndrome.

The fragile X syndrome (FXS), the most common cause of hereditary mental retardation, is caused by expansions of CGG repeats in the FMR1 gene. The gold-standard method to diagnose FXS is the Southern blot (SB). Because SB is laborious and costly, some adaptations in the polymerase chain reaction (PCR) method have been utilized for FXS screening.

In vitro trypanocidal activity of phenolic derivatives from Peperomia obtusifolia.

The trypanocidal activity of crude extracts and fractions from the leaves and stems of Peperomia obtusifolia (Piperaceae) was evaluated in vitro against the epimastigote forms of Trypanosoma cruzi. Bioactivity-guided fractionation of the most active extracts afforded seven known compounds, including three chromanes, two furofuran lignans and two flavone C-diglycosides. The most active compounds were the chromanes peperobtusin A and 3,4-dihydro-5-hydroxy-2,7-dimethyl-8-(2''-methyl-2''-butenyl)-2-(4'-methyl-1',3'-pentadienyl)-2 H-1-benzopyran-6-carboxylic acid, with IC (50) values of 3.