Efficiency of acetate-based isopropanol synthesis in Escherichia coli W is controlled by ATP demand.

Background: Due to increasing ecological concerns, microbial production of biochemicals from sustainable carbon sources like acetate is rapidly gaining importance. However, to successfully establish large-scale production scenarios, a solid understanding of metabolic driving forces is required to inform bioprocess design. To generate such knowledge, we constructed isopropanol-producing Escherichia coli W strains.

Increasing ATP turnover boosts productivity of 2,3-butanediol synthesis in Escherichia coli.

Background: The alcohol 2,3-butanediol (2,3-BDO) is an important chemical and an Escherichia coli producer strain was recently engineered for bio-based production of 2,3-BDO. However, further improvements are required for realistic applications.

Results: Here we report that enforced ATP wasting, implemented by overexpressing the genes of the ATP-hydrolyzing F-part of the ATPase, leads to significant increases of yield and especially of productivity of 2,3-BDO synthesis in an E.

Microbial Upgrading of Acetate into Value-Added Products-Examining Microbial Diversity, Bioenergetic Constraints and Metabolic Engineering Approaches.

Ecological concerns have recently led to the increasing trend to upgrade carbon contained in waste streams into valuable chemicals. One of these components is acetate. Its microbial upgrading is possible in various species, with being the best-studied.

Microbial upgrading of acetate into 2,3-butanediol and acetoin by W.

Background: Acetate is an abundant carbon source and its use as an alternative feedstock has great potential for the production of fuel and platform chemicals. Acetoin and 2,3-butanediol represent two of these potential platform chemicals.

Results: The aim of this study was to produce 2,3-butanediol and acetoin from acetate in W.

The Effects of Lactose Induction on a Plasmid-Free T7 Expression System.

Recombinant production of pharmaceutical proteins like antigen binding fragments (Fabs) in the commonly-used production host presents several challenges. The predominantly-used plasmid-based expression systems exhibit the drawback of either excessive plasmid amplification or plasmid loss over prolonged cultivations. To improve production, efforts are made to establish plasmid-free expression, ensuring more stable process conditions.