Implementation of a cost-effective HPLC/UV approach for routine medical quantification of memantine in human serum.

Background: In the near future, there will be no innovative drug principle for the treatment of dementia. Therefore, optimizing the efficacy of a drug is at present the most promising way to exploit its full pharmacological potential.

Method: A high performance liquid chromatography with ultraviolet assay for memantine in serum from demented patients has been developed and validated.

Implementation of a cost-effective HPLC/UV-approach for medical routine quantification of donepezil in human serum.

A novel, simple, specific and sensitive high performance liquid chromatography (HPLC) assay for the detection and quantification of donepezil in serum of demented patients has been developed and validated. The analytical procedure involves an offline serum preextraction using solid phase extraction (SPE) cartridges (Oasis® HLB, Waters Co). The chromatographic analyses were performed on a Dionex HPLC system with a Phenomenex Luna Phenyl-Hexyl analytical column, and a mobile phase with the two components 0.