Deciphering the impact of cold-adapted bioinoculants on rhizosphere dynamics, biofortification, and yield of kidney bean across varied altitudinal zones.

Agriculture stands as a thriving enterprise in India, serving as both the bedrock of economy and vital source of nutrition. In response to the escalating demands for high-quality food for swiftly expanding population, agricultural endeavors are extending their reach into the elevated terrains of the Himalayas, tapping into abundant resources for bolstering food production. Nonetheless, these Himalayan agro-ecosystems encounter persistent challenges, leading to crop losses.

Comparative e-waste plastics biodegradation efficacy of monoculture strain PE10 and bacterial consortium under condition.

A significant amount of electronic obsoletes or electronic waste (e-waste) is being generated globally each year; of these, ~20% of obsolete electronic items have plastic components. Current remediation practices for e-waste have several setbacks due to its negative impact on the environment, agro-ecosystem, and human health. Therefore, comparative biodegradation studies of e-waste plastics by monoculture strain PE10 and bacterial consortium consisting of strain PE2 (MF943156), strain PE5 (MF943157), PE8 (CP043311), and strain PE15 (MF943160) were carried out .

Microbial bioformulation: a microbial assisted biostimulating fertilization technique for sustainable agriculture.

Addressing the pressing issues of increased food demand, declining crop productivity under varying agroclimatic conditions, and the deteriorating soil health resulting from the overuse of agricultural chemicals, requires innovative and effective strategies for the present era. Microbial bioformulation technology is a revolutionary, and eco-friendly alternative to agrochemicals that paves the way for sustainable agriculture. This technology harnesses the power of potential microbial strains and their cell-free filtrate possessing specific properties, such as phosphorus, potassium, and zinc solubilization, nitrogen fixation, siderophore production, and pathogen protection.

Credibility assessment of cold adaptive MP1 and N26 on growth, rhizosphere dynamics, nutrient status, and yield of the kidney bean cultivated in Indian Central Himalaya.

Kidney bean () productivity and nutritional quality are declining due to less nutrient accessibility, poor soil health, and indigent agronomic practices in hilly regions, which collectively led to a fall in farmer's income, and to malnutrition in consumers. Addressing such issues, the present investigation was designed to assess the impact of MP1 and N26 treatment on soil health, microbial shift, yield, and nutrient status of the kidney bean in the Harsil and Chakrata locations of Indian Central Himalaya. MP1 and N26 were characterized as cold adaptive PGPR as they possessed remarkable plant growth promoting traits.

Leveraging arsenic resistant plant growth-promoting rhizobacteria for arsenic abatement in crops.

Arsenic is a toxic metalloid categorized under class 1 carcinogen and is detrimental to both plants and animals. Agricultural land in several countries is contaminated with arsenic, resulting in its accumulation in food grains. Increasing global food demand has made it essential to explore neglected lands like arsenic-contaminated lands for crop production.

sp. nov., isolated from a bacterial consortia of contaminated soil enriched for the remediation of e-waste.

A novel e-waste-degrading strain, PE08, was isolated from contaminated soil collected from a paper mill yard in Lalkuan, Uttarakhand, India. Strain PE08 was Gram-stain-negative, rod-shaped, aerobic, oxidase-positive and catalase-positive. Optimum growth was observed at 30 °C (range, 5-40 °C), with 1-2 % NaCl (range, 0-3 %) and at pH 7 (range 6-11).

Psychrophilic Pseudomonas helmanticensis proteome under simulated cold stress.

Himalayan mountains are distinctly characterized for their unique climatic and topographic variations; therefore, unraveling the cold-adaptive mechanisms and processes of native life forms is always being a matter of concern for scientific community. In this perspective, the proteomic response of psychrophilic diazotroph Pseudomonas helmanticensis was studied towards low-temperature conditions. LC-MS-based analysis revealed that most of the differentially expressed proteins providing cold stress resistance were molecular chaperons and cold shock proteins.

Differential protein profiling of soil diazotroph Rhodococcus qingshengii S10107 towards low-temperature and nitrogen deficiency.

Protein-based biomarkers can be a promising approach for identification and real-time monitoring of the bio-inoculants employed under sustainable agricultural plans. In this perspective, differential proteomics of psychrophilic diazotroph Rhodococcus qingshengii S10107 (JX173283) was performed to unravel its adaptive responses towards low-temperature nitrogen deficiency and identification of a biomarker for respective physiological conditions. LC-MS/MS-based proteome analysis mapped more than 4830 proteins including 77 up-regulated and 47 down-regulated proteins (p ≤ 0.

Towards the development of a biobased economy in Europe and India.

India has emerged as a key player with a high potential to develop a biomass and biobased economy due to its large geographic size and the massive amounts of agricultural and non agricultural biomass produced. India has joined hands with Europe to synchronize its efforts to create and facilitate the development of a biobased economy in this country. This paper aims to examine common research and development actions between the European Union (EU) and India to facilitate the development of these biobased economies.

Microbial diversity and soil physiochemical characteristic of higher altitude.

Altitude is the major factor affecting both biodiversity and soil physiochemical properties of soil ecosystems. In order to understand the effect of altitude on soil physiochemical properties and bacterial diversity across the Himalayan cold desert, high altitude Gangotri soil ecosystem was studied and compared with the moderate altitude Kandakhal soil. Soil physiochemical analysis showed that altitude was positively correlated with soil pH, organic matter and total nitrogen content.

Selection of potential bacterial strains to develop bacterial consortia for the remediation of e-waste and its in situ implications.

The management of electronic waste (e-waste) becomes a global issue in this digital era. Existing conventional practices are harmful for dealing with the e-waste, therefore, indigenous soil bacteria were explored for e-waste treatment through enrichment culture approach followed by screening, identification and their bioformulation used for in situ investigation. Soil bacteria were enriched in the presence of e-waste after 30 days of incubation under standard laboratory conditions.

Quantitative proteomics of psychotrophic diazotroph in response to nitrogen deficiency and cold stress.

Unlabelled: Effective protocols and novel biomarkers are the need of this hour to screen potential cold adapted diazotrophs for sustainable mountain agricultural plans. LC-MS/MS based gel less quantitative proteomics was employed to investigate the metabolic response of Himalayan cold adapted diazotroph Pseudomonas palleroniana N26 (JN055435) for nitrogen deficiency and cold stress. More than 5000 proteins were identified, and 125 of them showed significant difference with a 2-fold or greater change (p < .

Comparative in situ biodegradation studies of polyhydroxybutyrate film composites.

Application of polyhydroxybutyrate (PHB) to plastic industry has expanded over the last decades due to its attracting features over petro-based plastic, and therefore, its waste accumulation in nature is inevitable. In the present study, a total of four bacterial strains, viz., MK3, PN12, PW1, and Lna3, were formulated into a consortium and subsequently used as biological tool for degradation of biopolymers.

Isolation and characterization of phosphate solubilizing bacteria from Western Indian Himalayan soils.

Previous studies confirmed the existence of diversified microbial flora in the rhizosphere of Himalayan Red Kidney Bean (RKB) (Phaseolus vulgaris L.). Therefore, fifteen different temperate and subtropical regions of Western Indian Himalaya (WIH) were explored for the isolation of RKB rhizosphere-associated Phosphorus (P) solubilizing bacteria.

Cold Stress and Nitrogen Deficiency Affected Protein Expression of Psychrotrophic B2 and MP1.

Nitrogen (N) deficiency and low temperature conditions are the prominent facet of Western Himalayan agro-ecosystems. A slight change in the environment alters the protein expression of the microorganisms. Therefore, proteomes of the two psychrotrophs B2 and MP1 were analyzed using two dimensional electrophoresis and MALDI-TOF-MS, to determine the physiological response of altitudinally different but indigenous microorganisms in response to cold stress under N depleting conditions.

Exploration of nifH gene through soil metagenomes of the western Indian Himalayas.

This group has previously highlighted the prevalence of Csp genes from cold Himalayan environments. However, this study has explored the uncultured diazotrophs from metagenomes of western Indian Himalayas. The metagenomic nifH gene clone library was constructed from the Temperate, Subtropical and Tarai soils of Western Himalaya, India followed by polymerase chain reaction (PCR) amplification.

Response of indigenously developed bacterial consortia in progressive degradation of polyvinyl chloride.

Thermoplastic-based materials are recalcitrant in nature, which extensive use affect environmental health. Here, we attempt to compare the response of indigenously produced bacterial consortium-I and consortium-II in degrading polyvinyl chloride (PVC). These consortia were developed by using different combination of bacterial strains of Pseudomonas otitidis, Bacillus cereus, and Acanthopleurobacter pedis from waste disposal sites of Northern India after their identification via 16S rDNA sequencing.

Differential proteomic expression of himalayan psychrotrophic diazotroph Pseudomonas palleroniana N26 under low temperature diazotrophic conditions.

Background: In low temperature nitrogen-deficient ecosystems, native microorganisms must possess adaptive mechanisms to cope with environmental stress as well as nitrogen (N) starvation-like conditions. However, moderate information is available about the cold adapted diazotrophs and diazotrophy.

Objective: The aim of this study was to examine the proteomic response(s) of Himalayan psychrotrophic diazotroph under low temperature nitrogen fixing conditions.

Diversified diazotrophs associated with the rhizosphere of Western Indian Himalayan native red kidney beans (Phaseolus vulgaris L.).

Red kidney beans (RKBs) are one of the major components in the human diet of Western Indian Himalaya (WIH). Their cultivation in these habitats is strongly influenced by various biotic and abiotic stresses and therefore, there must be a selection of RKB associated microorganisms that are adapted to these harsh conditions. Seven cold adaptive diazotrophs from the same rhizosphere were isolated in our previous study to reveal the low-temperature associated proteins and mechanisms.

Growth promotory potential of the cold adapted diazotroph Pseudomonas migulae S10724 against native green gram (Vigna radiata (L.) Wilczek).

It is being confirmed previously the atmospheric nitrogen fixing ability of the cold adapted Pseudomonas migulae S10724 strain at the fluctuating temperatures. Therefore, net house bioinoculation experiment was performed to determine the effectiveness of inoculation of strain S10724 on the growth enhancement of native green gram (Vigna radiata L. Wilczek).

Differential proteomics in response to low temperature diazotrophy of Himalayan psychrophilic nitrogen fixing Pseudomonas migulae S10724 strain.

Himalayas are considered as a reservoir of diversified and dynamic gene pool. This study describes the response of a Himalayan psychrophilic diazotroph to low temperature diazotrophy. Seven cold adaptive N2 fixing bacteria were isolated and identified as Bacillus sp.

In vitro assessment of Ag2O nanoparticles toxicity against Gram-positive and Gram-negative bacteria.

In view of antibiotic resistance among pathogens, the present study is to address the toxicity of Ag2O nanoparticles against the Gram-positive and Gram-negative bacteria through in vitro assays. The preliminary screening by agar diffusion assay confirms the antibacterial activity of Ag2O nanoparticles against all the test bacteria. Comparative antibacterial activity of Ag2O nanoparticles and respective antibiotics reveals their broad range of activity and lower inhibitory dose against the used bacterial strains.

Expression of CspE by a psychrotrophic bacterium Enterobacter ludwigii PAS1, isolated from Indian Himalayan soil and in silico protein modelling, prediction of conserved residues and active sites.

Proteome analysis of Enterobacter ludwigii PAS1 provide a powerful set of tool to study the cold shock proteins along with that combination of bioinformatics is useful for interpretation of comparative results from many species. There is a considerable interest in the use of psychrotrophic bacteria for nitrogen fixation, especially at hilly regions, thus better understanding of cold adaptation mechanisms too. The psychrotrophic E.

Diversification of nitrogen fixing bacterial community using nifH gene as a biomarker in different geographical soils of Western Indian Himalayas.

Six soil samples (Pantnagar, Chamoli, Almora, Ranichauri, Pithoragarh and Badrinath) belonging to different geographical locations of Western Himalayas in India, were analyzed to diversify the nitrogen fixing bacterial community using nifH gene biomarker DNA from soil samples were isolated and amplified using nifH gene specific primers. Genomic DNA and PCR amplified products were then individually subjected to restriction digestion with tetra to octacutter enzymes (AluI, MspI, BgIII, XbaI, HindIII, HaeIII, AluI, MspI and PasI. Further restriction pattern was studied by preparing dendograms on the basis of similarity matrix and compared for the nifH community.