Genome-Guided Identification of Surfactin-Producing AQ11M9 with Anti- Potential.

The emergence of multidrug-resistant fungi is a worldwide health crisis connected with high rates of mortality. There is a critical need to find novel and unique antifungal compounds for treating infections of multidrug-resistant fungi such as . This study aimed to illustrate that biosynthetic gene clusters in native bacterial isolates are able to produce antifungal compounds against the multidrug-resistant fungus It was successfully achieved using large-scale antifungal activity screening, cytotoxicity analysis, and whole genome sequencing integrated with genome mining-guided analysis and liquid chromatography-mass spectrometry (LC/MS).

The Potential of Dutasteride for Treating Multidrug-Resistant Infection.

Novel antifungal drugs are urgently needed to treat candidiasis caused by the emerging fungal multidrug-resistant pathogen . In this study, the most cost-effective drug repurposing technology was adopted to identify an appropriate option among the 1615 clinically approved drugs with anti- activity. High-throughput virtual screening of 1,3-beta-glucanosyltransferase inhibitors was conducted, followed by an analysis of the stability of 1,3-beta-glucanosyltransferase drug complexes and 1,3-beta-glucanosyltransferase-dutasteride metabolite interactions and the confirmation of their activity in biofilm formation and planktonic growth.

Phenomics and genomic features of Enterococcus avium IRMC1622a isolated from a clinical sample of hospitalized patient.

Background: Enterococcus avium (E. avium) is a Gram-positive nosocomial pathogen that is commonly isolated from the alimentary tract. The objective of this functional genomics study was to identify the resistant genes by analyzing the genome of E.

Epidemiological characteristics of Klebsiella ozaenae infection and its antibiotic susceptibility: Experience of a tertiary care hospital in the Eastern Province of Saudi Arabia.

Background: (. ), a forgotten pathogen that normally colonizes the upper respiratory mucosa, can be associated with severe and invasive infections. The objectives of this study were to determine the frequency of isolation of .

Genomic Landscape of Multidrug Resistance and Virulence in IRMC827A from a Long-Term Patient.

We report on a highly virulent, multidrug-resistant strain of IRMC827A that was found colonizing a long-term male patient at a tertiary hospital in Khobar, Saudi Arabia. The IRMC827A strain carries several antimicrobial drug resistance genes and harbours mobile genetic elements such as Tn6009, which is an integrative conjugative element that can transfer resistance genes between bacteria and ISS1N via an insertion sequence. Whole-genome-sequencing-based antimicrobial susceptibility testing on strains from faecal samples revealed that the isolate IRMC827A is highly resistant to a variety of antibiotics, including tetracycline, doxycycline, minocycline, dalfopristin, virginiamycin, pristinamycin, chloramphenicol, streptomycin, clindamycin, lincomycin, trimethoprim, nalidixic acid and ciprofloxacin.

Mining the nanotube-forming MR14M3 genome for determining anti- and anti- potential by pathogenicity and comparative genomics analysis.

There is a global health concern associated with the emergence of the multidrug-resistant (MDR) fungus , which has significant mortality rates. Finding innovative and distinctive anti- compounds is essential for treating infections caused by MDR . A bacterial strain with anti- activity was isolated and identified using gene sequencing.

Genomic Insights into Virulence Factors and Multi-Drug Resistance in IRMC2505A.

is a spore-forming, Gram-positive anaerobic pathogen that causes several disorders in humans and animals. A multidrug-resistant strain was isolated from the fecal sample of a patient who was clinically suspected of gastrointestinal infection and had a recent history of antibiotic exposure and diarrhea. The strain was identified by 16s rRNA sequencing as .

Molecular identification of species in phlebotomine sand flies from Al Ahsa, Eastern KSA.

Objectives: Cutaneous leishmaniasis (CL) is considered an overlooked public health threat in KSA. CL is endemic to several Saudi regions, including Al Ahsa City, Eastern KSA. To our knowledge, no prior molecular identification of parasites in vectors in Al Ahsa has been published.

Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite.

(), an emerging multidrug-resistant microorganism, with limited therapeutical options, is one of the leading causes of nosocomial infections. The current study includes 19 strains collected from King Fahd Hospital of the University and King Fahad Specialist Hospital in Dammam, identified by gene and region sequencing. Drug-resistance-associated mutations in genes were screened to gain insight into the pattern of drug resistance.

Silver Nanoparticles: A Promising Antifungal Agent against the Growth and Biofilm Formation of the Emergent .

Candida auris is a globally-emerging pathogen that is correlated to nosocomial infections and high mortality rates, causing major outbreaks in hospitals and serious public health concerns worldwide. This study investigated the antifungal activity of silver nanoparticles (AgNPs) on clinical isolates of C. auris.

Genotypic and phylogenic analyses of cutaneous leishmaniasis in Al Ahsa, Eastern Saudi Arabia during the coronavirus disease 2019 pandemic: First cases of Leishmania tropica with the predominance of Leishmania major.

During the coronavirus disease 2019 lockdown period, a surge in sandflies and cutaneous leishmaniasis (CL) cases was observed in Al-Ahsa, Saudi Arabia. Skin punch biopsies were obtained from 100 patients clinically diagnosed with CL in Al-Ahsa who had no travel history in the last 6 months. Impression smears were used following a three-step polymerase chain reaction (PCR) protocol using genus-specific primers targeting kDNA and ITS1.

Sensitivity and Specificity of Rapid SARS-CoV-2 Antigen Detection Using Different Sampling Methods: A Clinical Unicentral Study.

Rapid antigen detection of SARS-CoV-2 has been widely used. However, there is no consensus on the best sampling method. This study aimed to determine the level of agreement between SARS-CoV-2 fluorescent detection and a real-time reverse-transcriptase polymerase chain reaction (rRT-PCR), using different swab methods.

Anti-blastocystosis activity of antioxidant coated ZIF-8 combined with mesoporous silicas MCM-41 and KIT-6.

The biocompatible hybrid Zeolitic imidazolate framework-8 (ZIF-8)/structured silica nanocomposite can be loaded with antioxidants such as curcumin and resveratrol to offer multiple advantages of drug functionalization and structural stability. blastocystosis, an enteric parasite, has various outcomes and its treatment includes drugs which have side effects and do not result in a full cure. We aimed to design novel biocompatible nanocomposites containing natural antioxidant, resveratrol or curcumin and ZIF-8/mesoporous silica.

A decade of travel-associated malaria at King Fahad Hospital of the University in the Eastern Province of Saudi Arabia.

Travel-associated malaria is a health hazard, even in non-malaria endemic regions. This is a hospital-based retrospective study of 12,931 febrile patients who presented at King Fahad Hospital of the University (KFHU) from January 2009 to December 2019. Patients either returning from malaria endemic countries and/or for whom malaria was suspected, had blood films microscopically screened for malaria parasites.

Microleakage and Bacterial Adhesion with Three Restorative Materials Used to Seal Screw-access Channels of Implant Abutments: An Study.

Background: Proper sealing of screw-access channels against microbial microleakage is advisable for the long-term success of screw-retained implant prosthesis.

Objective: This study aimed to compare the bacterial adhesion and microleakage with three restorative materials, namely, composite resin, acrylic resin and bis-acryl, that are used to cover the access channels of screw-retained implant prostheses, using polytetrafluoroethylene tape as a spacer material.

Materials And Methods: In this study, 18 titanium straight abutments (Hex-lock Zimmer) were torqued into implant analogs, which were then subdivided into three groups.

Antimicrobial resistance trends of non-fermenter Gram negative bacteria in Saudi Arabia: A six-year national study.

Background: Antimicrobial resistance (AMR) of non-fermenting Gram-negative bacteria (NFGNB) is increasingly recognized as urgent healthcare threat. Trend data on AMR of NFGNB in Saudi Arabia are either old or limited. The objective was to estimate the prevalence and resistance trends of isolated NFGNB in Saudi Arabia.

Diagnostic deficiencies of infection among patients in a tertiary hospital in Saudi Arabia: A laboratory-based case series.

infection (CDI) has become a threatening public health problem in the developed world. In the kingdom of Saudi Arabia, prevalence of CDI is still unknown due to limited surveillance protocols and diagnostic resources. We used a two-step procedure to study and confirm cases.

Development of multiplex real-time RT-PCR assay for the detection of SARS-CoV-2.

The outbreak of the new human coronavirus SARS-CoV-2 (also known as 2019-nCoV) continues to increase globally. The real-time reverse transcription polymerase chain reaction (rRT-PCR) is the most used technique in virus detection. However, possible false-negative and false-positive results produce misleading consequences, making it necessary to improve existing methods.

Evaluation of Bacterial Leakage in Platform-Switching Dental Implant with Morse Taper Connection Under Thermocycling and Loading Effects: In Vitro Study.

Purpose: The aim of this study was to test in vitro the bacterial microleakage at the Morse taper implant-abutment connection with switched platform subjected to functional load and thermocycling.

Materials And Methods: A total of 48 Morse taper implant-abutment connections with switched platforms (12 implants per group) were used. The abutments were attached to implants and presented in four groups: group 1, control; group 2, subjected to thermocycling; group 3, subjected to cyclic compressive loading; and group 4, subjected to thermocycling and cyclic compressive loading.

Two Cases of the Emerging in a university hospital from Saudi Arabia.

is an opportunistic multidrug-resistant pathogen that was first isolated in 2009 and has since been reported from about 30 countries. In Saudi Arabia, only four cases of have previously been reported; here, we report two new cases of this infection. Both patients were polymorbid and had long hospitalization periods with recurrent intensive care unit (ICU) admissions.

Scenario of pathogenesis and socioeconomic burden of human brucellosis in Saudi Arabia.

Brucellosis is considered a prevailing endemic infectious disease in the Kingdom of Saudi Arabia and represents a health problem with socio-economic burden. There are two main species that cause human brucellosis; , and . The clinical features range from asymptomatic to the acute symptoms of fever, joint pain, muscle pain, headache, nausea/vomiting, anorexia and malaise in addition to the subsequent complications that might occur.

Drug Resistance-Associated Mutations in of Multidrug-Resistant in a Tertiary Care Hospital of Eastern Saudi Arabia.

is an emerging multi-drug resistant pathogen with high mortality rate; nosocomial infections have been reported worldwide, causing a major challenge for clinicians and microbiological laboratories. The study aims to describe new cases of and detect drug resistance-associated mutations of by the sequencing of and genes. A total of six specimens were collected from blood, urine, ear swab, and groin screening samples.

Using for bioinspired synthesis of titanium dioxide and silver nanoparticles, targeting biomedical applications.

The current study proposes a bio-directed approach for the formation of titanium oxide and silver nanoparticles (TiO and Ag NPs), using a wild mushroom, , identified by 18S ribosomal RNA gene sequencing (gene accession no. MK635350) and phenotypic examination. NP synthesis was confirmed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), diffuse reflectance UV-visible spectroscopy (DR-UV), and scanning and transmission electron microscopy (SEM/TEM).

A Wild for Biomediation of One Pot Synthesis of Titanium Oxide and Silver Nanoparticles for Antibacterial and Anticancer Application.

The present study offers an alternative method for green synthesis of the formation of two types of nanoparticles (NPs). These NPs, titanium oxide and silver NPs (TiO and Ag NPs, respectively), were obtained from the amalgamation of intracellular extract of a wild mushroom, with aqueous solutions of titanium isopropoxide and silver nitrate, respectively. was identified phenotypically and by 18S ribosomal RNA gene sequencing (Gene accession no: MK635351).

Anticandidal and In vitro Anti-Proliferative Activity of Sonochemically synthesized Indium Tin Oxide Nanoparticles.

The present work demonstrates the synthesis, characterization and biological activities of different concentrations of tin doped indium oxide nanoparticles (Sn doped InO NPs), i.e., (Sn/In = 5%, 10% and 15%).