Publications by authors named "Recep Hakkı Koca"
The objective of this study was to examine the impact of freeze-thawing on the levels of oxidative stress, fatty acids, vitamins A, D, E, and K, cholesterol, and amino acids, as well as on spermatological parameters, in ram semen. Semen was collected and pooled from each of the seven rams twice a week for three weeks. The mixed semen was diluted with tris + egg yolk diluent at 38 °C (Group 38 °C) and the temperature was reduced to 5 °C (Group 5 °C).
View Article and Find Full Text PDFArticle Synopsis
- The study explored the effects of hydrated carbon 60 fullerene (C HyFn) on frozen-thawed ram sperm, assessing morphological, functional, and molecular changes.
- Semen from seven Akkaraman rams was pooled, diluted with varying C HyFn concentrations, frozen, and analyzed for motility and molecular markers.
- Results showed that C HyFn significantly improved sperm motility and mitochondrial activity while reducing sperm damage and specific gene expressions, suggesting its potential in enhancing semen quality post-freezing.
Article Synopsis
- The study investigated how sulforaphane (SFN) affects the cryopreservation of rabbit semen by dividing samples into control and varying SFN concentrations (5, 10, 25, 50 µM).
- Results showed that while there was no difference at 4°C, the 10 µM SFN group exhibited the best outcomes in terms of sperm motility and lower rates of acrosomal damage after freezing and thawing.
- Overall, the findings suggest that a 10 µM dose of SFN enhances sperm quality in the cryopreservation process for rabbits.
This study was carried out to investigate the effect of the semen freeze-thawing process on the functionality and molecular structure of ram spermatozoa. The temperature of pooled and diluted semen at 38°C (group 1, control) was lowered to 5°C (group 2), and it was subjected to glycerolisation-equilibration (group 3), frozen and thawed (group 4). Compared to the control, deterioration in spermatological parameters and significant increases in lipid peroxidation and global DNA methylation levels were observed in groups 3 and 4.
View Article and Find Full Text PDFArticle Synopsis
- The study aimed to assess how hydrated carbon 60 fullerene (CHyFn) affects the quality of ram semen during the freezing and thawing process.
- Semen from seven Akkaraman rams was collected and treated with different concentrations of CHyFn before freezing, and key parameters like motility and oxidative stress were measured post-thawing.
- Results indicated that CHyFn improved motility and membrane integrity while reducing sperm damage and oxidative stress, suggesting it could be beneficial for preserving ram semen quality.