Publications by authors named "Rebone Moerane"

Article Synopsis
  • The study focused on characterizing a common bacterial pathogen found in beef and its products using whole-genome sequencing (WGS) on 24 isolates.
  • The most prevalent sequence type was identified as ST204, along with several others, and many isolates carried genes related to virulence and antibiotic resistance.
  • Key findings emphasized the potential risk this bacterium poses to public health due to its presence in food environments, highlighting both its virulence factors and environmental resistance traits.
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Article Synopsis
  • The study analyzed 60 bacterial isolates from the beef production chain in Gauteng, South Africa, using whole-genome sequencing and bioinformatics to determine their genomic characteristics.
  • Researchers identified seven sequence types (STs) and six clonal complexes (CCs), revealing that ST204 and CC204 were the most prevalent among the samples.
  • The investigation uncovered 44 virulence factors, two antimicrobial resistance (AMR) genes, and highlighted that certain genes associated with virulence and resistance were present across all isolates, indicating potential therapeutic concerns for these pathogens.
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Article Synopsis
  • * A study found a 28% prevalence of Listeria spp. in various beef products in Gauteng, with pathogenic serogroups 4b-4d-4e and 1/2a-3a identified, which carry virulence-associated genes.
  • * The research highlighted that regional factors, product type, and storage temperature influenced Listeria occurrence and stressed the need for improved food safety measures to prevent future outbreaks.
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Article Synopsis
  • Whole-genome sequencing was performed on 110 strains of bacteria isolated from cattle farms, abattoirs, and retail outlets in Gauteng, South Africa, to analyze their genetic makeup.
  • The study identified 14 distinct sequence types (STs) and found that all strains carried antimicrobial resistance genes, with 13 specific resistance genes and 23 virulence genes detected across all isolates.
  • The findings highlight significant diversity in the genetic profiles based on the sources and types of samples, marking the first genomic characterization of these bacteria in South Africa's beef production system and raising concerns about food safety.
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Article Synopsis
  • - The study analyzed 60 Listeria monocytogenes isolates from various sources in Gauteng, South Africa, using multiple-locus variable-number analysis (MLVA) to determine their genotypes and serogroups.
  • - The sources included raw beef, ready-to-eat products, carcass swabs, cattle environments, and feces, with findings showing significant distribution across these sample types.
  • - MLVA successfully identified genetic relations and differences among the isolates, revealing distinct patterns in tandem repeats (TRs) and leading to the identification of at least five MLVA types in the region.
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Article Synopsis
  • Previous studies in Limpopo Province did not cover all districts or seasonal patterns of gastrointestinal nematodes (GIN), making current data on prevalence and anthelmintic resistance (AR) crucial for understanding sheep health in the region.
  • The research involved fecal egg count reduction tests and various laboratory methods to assess AR against common dewormers, sampling 780 infected sheep across five districts.
  • Results indicated a high prevalence of GIN (88-100% during hot wet season) and significant resistance to certain anthelmintics, with indications of mild anemia among sheep based on FAMACHA scoring, particularly in the Vhembe district.
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Anthelmintic treatment is the most common way of controlling nematode infections in ruminants even though several countries have reported anthelmintic resistance (AR), resulting in limitation for sustainable small ruminant production. The aim of the present study was to evaluate the knowledge of resource-poor sheep farmers in Limpopo province of South Africa on the use of anthelmintics. A questionnaire regarding helminthosis control practices was administered to small ruminant farmers in five districts of Limpopo province namely Capricorn, Sekhukhune, Waterberg, Vhembe, and Mopani.

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