Optimizing of the basophil activation test: Comparison of different basophil identification markers.

Background: Flowcytometric identification of basophils is a prerequisite for measuring activation of basophils with IgE-dependent or IgE-independent stimuli. Aim of this study was to compare different marker combinations in a simultaneous multicolor flowcytometric measurement.

Methods: Ten patients with a grass pollen allergy and three controls were included in the study.

Optimizing of the basophil activation test: Comparison of different basophil identification markers.

Background Flowcytometric identification of basophils is a prerequisite for measuring activation of basophils with IgE-dependent or IgE-independent stimuli. Aim of this study was to compare different marker combinations in a simultaneous multicolor flowcytometric measurement. Methods Ten patients with a grass pollen allergy and 3 controls were included in the study.